Initial Issue Date: Section: Procedure:
Revision Date: Sub-Section: Surface Plasmon
Title: Standard Operating Procedure: Surface Plasmon Document Number:
Resonance for Biacore 1000 model.
Approved by: Page 1 of 3
This procedure outlines the steps to be followed when using the Biacore 1000
for surface plasmon resonance.
2.1 This procedure applies to all students, staff and researchers who wish to
use the Biacore.
Surface plasmon resonance - The excitation of surface plasmons (surface
electromagnetic waves) by light.
4.1 It is the responsibility of all students, staff and researchers to ensure they
receive proper hands-on training by staff of the facility before performing
the procedure on their own.
4.2 It is the responsibility of all students, staff and researchers to use the
Biacore according to the procedures enclosed in this document.
4.3 It is the responsibility of the USER to report any damage or malfunction of
this equipment to the emergency contact person listed. You are required to
leave a note on the equipment describing the problem and include your
name and date the problem occurred.
5.0 Related Policies/Procedures:
7.0 Action/Decision-making Framework:
PROCEDURE WORK INSTRUCTIONS RATIONALE
7.1 Pre-use check .1 Inspect electrical connections and hook up to
recommended electrical supply.
.2 Leak check liquid connections, pump
syringes & valves, injection port and
.3 Ensure fan inlet is unobstructed.
7.2 Instrument start up .1 The Biacore machine will be running with the
maintenance chip inside the instrument.
.2 If the Biacore instrument has been shut off
for some reason, turn it on (switch at the
back). It will take approximately 1 hour for the
temperature to stabilize (yellow light on the
front panel lights up steadily when it is
.3 You should be de-gassing your filtered (0.22
um) buffer upon entering the lab.
.4 Click the “exit” button to stop the continuous
.5 UNDOCK the maintenance chip by clicking
the “Command” tab then “Undock,” then
follow the prompts. Next, remove the
maintenance chip from the side of the
instrument and insert your maintenance chip.
Once inserted again click “Command,” then
“Dock” in the software to engage the sensor
chip. Follow the prompts.
.6 Run the PRIME procedure a couple of times
to ensure a stable baseline. Click Tools--
7.3 Operational notes .1 The Biacore 1000 works from a program
using MDL (Machine Definition Language).
Write the program needed for your samples
including regeneration cycles.
.2 Always have the command “Append
Continue” at the end of the program to put
the machine in standby mode which flows
buffer across all flowcells @ 5µL/mL for 72
.3 Always make sure there is enough buffer in
the reservoir for your runs and up to 72 hours
PROCEDURE WORK INSTRUCTIONS RATIONALE
7.4 Saving Data .1 It is highly recommended that you transfer
your data to your own computer on a regular
7.5 Equipment .1 Because this instrument has a multi-user
Cleaning / Shutdown environment, each user is responsible for
properly cleaning the instrument after they
are done with their experiments.
.2 Remove your sensor chip and dock the
.3 Run the DESORB cleaning procedure
(Tools>>Working Tools>>Desorb). Note: The
DESORB cleaning procedure is adequate for
most applications. However, additional
cleaning steps may need to be implemented,
depending on the system you are studying.
To remove microbial Desorb should be run weekly and Sanitize
contamination should be run monthly.
.4 Run the instrument check to ensure that the
instrument is running properly for the next
.5 Insert the maintenance chip and set the
instrument to Continuous flow
(Tools>>Working Tools>>Continue). Use
Millipore water as the flow solution.
7.6 Safety .1 All those using the Biacore 1000 must wear
the appropriate personal protective
equipment. This should include lab coats,
gloves and eye protection if necessary.
.2 Keep hands away from needle during
.3 Unplug before performing any maintenance
or service operation.
o Instrument Manual
o Template provided by Hamilton Health Sciences: Anatomical Pathology
Department- McMaster University Medical Center.
10.0 Developed By in Consultation With:
FHSc. Safety Office
Allan Stafford, HRC