ECL reagent

By Craig Bryant,2015-02-04 21:41
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Solution A: 100mM Tris-HCl (PH 8.5) (Stock solution 1M) 1.24mM Luminol (Stock solution, 250mM/DMSO) 0.0033% p-Coumaric acid (Stock solution, 1.5%/DMSO) Solution B: 30% H2O2 As the Luminol and p-Coumaric acid, you can make stock solution in DMSO. And the solution A need kept in brown bottle. When you do ECL reaction, you need mix 10mL..

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