By Craig Bryant,2015-02-04 21:41
100mM Tris-HCl (PH 8.5) (Stock solution 1M)
1.24mM Luminol (Stock solution, 250mM/DMSO)
0.0033% p-Coumaric acid (Stock solution, 1.5%/DMSO)
As the Luminol and p-Coumaric acid, you can make stock solution in DMSO. And the
solution A need kept in brown bottle.
When you do ECL reaction, you need mix 10mL..