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THE ABILITY OF SOIL BACTERIM ENTEROBACTER NIMIPRESSURALIS 32-3 TO ...

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THE ABILITY OF SOIL BACTERIM ENTEROBACTER NIMIPRESSURALIS 32-3 TO ...

THE ABILITY OF SOIL BACTERIM ENTEROBACTER NIMIPRESSURALIS 32-3 TO

    SYNTHESIS OF PHYSIOLOGICAL ACTIVE COMPOUNDS (PHYTOHORMONES)

    Chaikovska L.A., Baranska M.I.

    South Experimental Station of Institute Agricultural Microbiology of Ukrainian Academy of

    Agricultural Sciences, K.Marx 107, Gvardeyskoe, Simferopol district, Crimea, Ukraine;

    e-mail:ludachaika@mail.ru

    Phytohormones have important role as regulators of growth and development of plants [1]. This compounds regulate the condition of rest and germination of seeds, influence on the roots-formation, flowering, branching, maturing of fruits, also increase the resistence of plants to factors of environmental. It is known three groups phytohormones, which improve growth and productivity of plants: auxin-line, gibberellin-line, citokinin-line. Synthesis of phytohormones are very important property of many bacterium of rhizosphere also epiphyting and symbiosing bacterium, so-called PGPB-strains, which stimulate and increase the growth of plants [2,3].

    In our researches was revealed the possibility of soil bacterium Enterobacter

    nimipressuralis 32-3 to synthesis(e) of physiological activity compounds in particular phytohormones. The excretion of physiological activity compounds were carried out in accordingly by generally accepted methods. Phytohormones from daily (twenty-four-hour) cultural liquid of bacterium E. nimipressuralis 32-3 have been excreted. In the capacity of test-

    plants were used winter wheat (cultivar Albatros odessky), mais (hybrid Rosava) and cucumber (cultivar Fenix). Our experiments were carried out in laboratory conditions.

    The results our experiments showed, that in cultural liquid of bacterium E.

    nimipressuralis 32-3 have been revealed number (three) phytohormones of various character. For determination of phytohormones by auxin-line were used the shoots of winter wheat. The greatest growth of length shoots in diluting extract of cultural liquid has been registred (fig.1). So in diluting by 1:500 and 1:1000 of

    140,0127,9123,1%116,9115,8116,2120,0109,9

    100,0

    100,0

    80,0

    60,0

     40,0Fig.1.The influence of extract by cultural liquid of E. пimipressuralis 32-3 at length shoots (%) of -4-5winter wheat (cultivar Albatros odessky); 1 control (water); 2 IAA 10; 3 - IAA 10; 20,0 4, 5, 6, 7 extract by cultural liquid: 1:100; 1:250; 1:500; 1:1000 in accordingly.

     0,0cultural liquid extracts were growth of shoots over 27,9% and 23,1% by comparison control; in 1234567diluting 1:100 and 1: 250 only 16% by comparison control (water). At the same time authentic -4-5compound the indoleaceto-acid (IAA10 or IAA10) provided only 10-16%.

    Identical results obtained for investigation of compounds by gibberellin-line (fig.2). The greatest growth of length shoot maize in diluting extract of cultural liquid E. nimipressuralis 32-

    3 has been registred. So, by dilute 1:250 and 1:1000 provided the growth of shoot mais over 17% and 31% accordingly by comparison control. At the same time authentic compound the -4gibberellyn acid (GI 10) provided 22% of growth shoots by comparison control.

    160,0

    %131,7140,0122,3117,0120,0108,4101,5100,0

    100,0

    80,0

    60,0

     40,0

    Fig.2. The influence of extract by cultural liquid of E. nimipressuralis 32-3 at length shoots (%) of 20,0-4maize (hybrid Rosava); 1 control (water); 2 GA 10; 3, 4, 5, 6 extract by cultural liquid:

    1:100; 1:250; 1:500; 1:1000 in accordingly. 0,0

     123456Our researches the presence in cultural liquid of E. nimipressuralis 32-3 the

    phytohormones by citokinin-line has been allowed (fig.3). As it is well known, 250

    206,1%200

    143,0137,7150

    101,0100,8100

    10084,8

    50

Fig.3. The influence of extract by cultural liquid of E. пimipressuralis 32-3 at growth of biomass (%) 0-4 -5leaves of cucumber (cultivar Fenix):1 control (water); 2 - BAP10, 3 - BAP10; 1234567 4, 5, 6, 7 extract by cultural liquid: 1:500; 1:1000; 1:2000; 1:4000 in accordingly.

this compounds stimulate of growth plants only in slightes quantity. Apparently, our bacterium E.

    nimipressuralis 32-3 produced enough great quantity of compounds by citokinin-line. So, only diluting of cultural liquid by 1:2000 or 1: 4000 the growth of biomass leaves of cucumber were registred: over 43% and 37,7% by comparison control accordingly. The authentic compound -4 benzapiren (BAP) reduced of biomass leaves of cucumber in diluting 10at 15% in comparison -5 control, but diluting of BAP at 10increased of this index at 106% in comparison control.

    Thus our researches revealed, that soil bacterium E. nimipressuralis 32-3 have ability to

    synthesis of physiological activity compounds in particular phytohormones. This compounds belong to three groups of phytohormones: auxin-line, gibberellin-line, citokinin-line. The laboratory experiments showed, that slightes quantity revealing compounds increase growth of length shoot of winter wheat and maize, also of biomass leaves of cucumber.

1. Derfling K. Hormones of plants. Systems approach. Moskau. 1985. 304p. (in rus.)

    2. Mishke I.V. Phytohormones of microbes to plant-growing. Riga. 1988. 151p. (in rus.)

    3. Tsavkelova E.A., Klimova S.Yu., Cherdintseva T.A., and Netrusov A.I. / Microbial producers of plant growth stimulators and their practical use: a review. J. of applied biochemistry and

    microbiology. - Moskau. 2006. 42, 2. p.133-143. (in rus.)

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