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Immobilization biological activated carbon used in advanced drinking water treatment

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Immobilization biological activated carbon used in advanced drinking water treatmentin,IN,used,water,USED,WATER,Water

    Immobilization biological activated carbon used in advanced drinking water treatment JournalofHarbinInstituteofTechnology(NewSeries),Vo1.13,No.6,2006 Immobilizationbiologicalactivatedcarbonusedin

    advanceddrinkingwatertreatment

    MAFang,rANGttaiyan,WANGyM

    马放,杨海燕,王弘宇

    (SchoolofMunicipalandEnvironmentalEngineering,ttarhinInstituteofTechnology,t|arhinI50090,China,E-mail:haiyanyang.hw@sina.corn)

    Abstract:Bacteriaseparatedfromamaturefilterbedofgroundwatertreatmentplantswerejncuhatedinacu1.

    1uremediacontainingironandmanganese.Aconsortiumof5strainsofbacteriaremovingironandmanganese

    wereobtainedbyrepeatedenrichmentcuhuring,Itwasshownfi'olfltheexperimentsofe1]betfactorsthatiron.

    manganeseremora1bacteriaintheculturemediacontainingbothFean(1Mngrew1)etterthaninthatcontaining

    onlyFe,however,theywereunabletogrowintheculturemediacontainingonlyMn.Whencomparingthebac-

    teriabiomassinthecaseofp(DO)=2.8mg/Landp(DO)=9,0mg/L,nosigniticantdifferencewasfound.

    Theengineeringbacteriaremovingtheorganicandthebacteriaremovingironan(Jmanganeseweresinmhaneous-

    lYinoculatedintoactivate(1carbonreactortotreattheeffluentofdistributionnetwol?k.Theexperimentalresults

    showedthatbyusingIBAC(ImmobilizationBMogicalActivate~1Carbon)treaanent.theremoralefficiencyofi-

    ron,manganeseandpermanganateindexwasnlorethan98%,96%and55%,respectively,Aftertheinfluent

    withturbidityof1.5NTU,colorof25degreeandoft~nsiveO(tOFwastreated.theturbiditYandcolorofeffluence

    werelessthan0.5NTUand15degree.respe(?lively.air1itWasodol-tess,Itisdetermine(1thatthecooperation

    funetionofengineeringbacteriaandactivatedcarbonachievedadvanceddrinkingwatel'lreatment,

    Keywords:advanceddrinkingwatertreatment;micro-pollutants;lBAC;ironant1manganeseremoval;engi-

    neeringbacteria

    CLCnumber:TU991.2Documentcode:AArticleID:l005.9l13(2006)06.0678-05 Ironandmanganeseenrichmentisaprimarytrait

    ofwaterqualityofgroundwaterinNorthChina..There.

    forethereisinnererosiontakingplaceinthewater

    pipesduringthedistributionofgroundwater.itnotonly

    makesthewaterqualityindex(turbidity,color,spe

    tiesofbacterial,iron,manganeseandpoisonousheavy

    metalconcentration)deteriorativebutalsoreducesthe

    waterdistributioncapacityowingtotheexistenceof

    "

    growthcircle"inthewaterpipes.Sothemainprot>

    lemishowtoremovetheironandmanganeseof

    groundwaterintheprocessot.groundwatertreatment.

    Recently,someresearchershaveraisedaview

    pointthatironbacteriaisanevocatingfactorforthefor

    mationofironandmanganeseremovalfilterfilmand

    subsequentlydevelopedaseriesofstudiesonironand

    manganeseremovalbybiologicalmethods.Inthis

    workwepresenttheuseofmicrobialimmobilization

    technologyforironandmanganeseremovalIlfgroundw- aterconsideringtheseconftpollutionofthewatersup

    plysystemcausedbygrowthcircles.Theiron-manga

    nesebacteriaobtainedbyartificialseparation.purifica

    lionandenrichmentareinoculatedintothefiltration bed.Alsothemuhinicheandthe("mditionparameters whichal-eagreeableforthebacteriatovegetateandto promoteitsactivityarediscussed.Inordertoprovide peoplewithhighqualitydrinkingwater,itisnecessary thatadvanceddrinkingwatertreatmentisusedtosolve theproblemofgrowthcircleandaccomplishironand manganeseremova1.

    1MaterialsandMethods

    1.1RawWaterintheExperiment

    Therawwaterusedintheexperimentistaken

    fromtheen(ieffluentofadistributionnetworkfroma certainwaterplantwhichusesgroundwaterasthewater sourceantiislcoatedahmgthebankoftheSonghua RiverinHarbinii1northeastChina.Becausethe groundwateriswatersourceandthereisinnererosion inthepipes,thewaterqualityoftheconsumerwateris veryunstableandsuchkindofwatermustbetreatedby advancedtreatment.Thewaterqualityduringtheex

    perimentislisletIinTab.1.

    Received20031219,

    SponsoredbytheNationalltighTechnoh)gyResearehandDevelopmentProgralnofChina((;

    rantNo.2002AA60112O)

    -

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JournalofHarbinInstituteofTechnology(NewSeries),Vo1.13,No.6,2006

    Tab.1Thewaterqualitycharacteristicsofendofwaterpipelinesample

    1.2ExperimentalApparatus

    Accordingtothetraitsofrawwaterquality,the mainobjectswehavetoremoveareiron,manganese, organicpollutants,turbidityandcolor.Itisnotedfrom Tab.1thatironandmanganeseconcentrationofwater iscomparativelynothigh,butastheeffluentofdistri- butionnetwork,theirconcentrationismorethanthe drinkingwaterqualitystandard.Aimingatthemicro- pollutedwateroflowironandmanganeseconcentra- tion,weusedIBACtotreatsuchkindofwaterbyad- vancedtreatmentandguaranteetheeffluentqualityis eligible.

    Fheprocessusedintheexperimentisshownin Fig.1.

    BAC_HUVdisinfe(?ti'm

    Fig.1Schematicdiagramoftheexperimentaltreatment IBACcolumnconsistedofa200cmhighplexi.

    glasscolumnwithaninsidediameterof30cm.in whichZJl5granularcarbono1.90cmthicknesswas loadedinternally.ItsHydraulicRetentionTime (HRT)was30minandfilterratewas11.2I/min.

    Theactivatedcarbonwasmarinatedandrinsedbytap watertilltheeffluentwasclear.Thenitwasfilledin thecolumntotheprescriptiveheightafterpouringits moisture.TheengineeringbacteriaIiquorwasinfused intothecarboncolumn.Atierthecarbon1ayerwassat. urated.theeffluentwasdischargedatarateofonere. cycleper4h.Thecarboncolumnwasrinsedbytap

    waterfor15minasthealternativeofthebiologicalac. tivatedcarboncolumnaftereach24hoperationcycle. ANLC008UVdisinfectiontubewasusedto

    killtheharrnfulmicroorganismsinthewaterbyultravi. oletradiation.ThesetuprequiredthatUV254ofthein- fluentwasmorethan95%andtheinfluentvolumetric flowratewaslessthan0.3t/h.

    1.3AnalyticalMethodsandReagents

    AlJtheironandmanganeseanalyseswereper

    formedusinganatomicabsorptionspectrophotometer (modelSpeetrAA10plus,VARIAN).Permanga-

    nateindexwasdeterminedwithaPHS25apparatus

    accordingtoaciditymeth('d.Theturbiditywasmeas

    uredwithaTURB555a1)I)?dtusfromGermany.

    A11reagentsusedintheexperimentwereofana. 1yticalgradewhileotherswerebiologicalreagents. 2ResultsandDiscussion

    Thereismuchstudyonimmobilizationbiological techniqueusedtoremovetheorganicsindrinkingwa. terl'.

    butthereisnotmuchstudyonironandman.

    ganeseremovalusingimmobilizationbiologicaltech- nique.Themainmethodsusedintheironandmanga- neseremovalarecontactoxidationprocesses.Whena filterbedwasoperatedforaperiodoftime,thefilter bed(manganesesandfiltermateria1)becamemature. Itwasconsideredthattherewasanactivefiltermem- braneofironandmanganeseoxideorironandmanga- neseoxidationbacteria,whichaccomplishedtheiton andmanganeseremovalinthefilterbed.Sowecom-

    binedaclassicaltheorywiththerisingbiologicalaug- mentationtechnique.adoptedartificialimmobilization techniqueandinoculatedtheiron.manganesebacteria obtainedbyartificialseparation.purificationanden. richmentintoactivatedcarbonbed.Bythis.theiton andmanganeseinwatercanberemovedtoachievead. vanceddrinkingwatertreatment.Inordertoremove theorganicsinwater.theengineeringbacterialwhich couldremovetheorganicshouldbesimultaneouslyim. mobilize(1intotheactivatedcarbon

    2.1FactorExperimentsofIronandManganese Remoral

    2.1.|Effectfactoranalysis

    Becausethebacterialspecieswasnotsingleinthe reactorduringtheactualengineeringapplication,5 strainsofironandmanganesedegradationbacteriaob- tainedbyseparatingandacclimatingweremixedto- gethertocultureinthestirringbed.The5strainsof bacteriaweredenominatedasFM1,FM2,FM3,FM4 andFM5respectively.

    Intheexperiment.threekindsofsubstrateinclu. dingdifferentingredientswerqconfected:1)thesub- strateonlyincludingironwithFeconcentrationof 22.06me,/L,2)thesubstrateonlyincludingmanga

    nesewithMnconeentrationof18.67mg/L.3)the

    substrateincludingironandmanganesewithFeandMn concentrationof40.73mg/L,inorganicsaltingredi- entswerethesameinthethreekindsofsubstratemedi. umabove.Thequantityofcultureliquorandbacteria inoculatedwerethesameinthesixconicalflasks.Two

    differentculturemodesofaerationandnoaerationwere providedanditwasfoundthatthedissolvedoxygen concentrationwasabout2.8me,/Lbeforeaerationand wasmorethan9me,/Lafteraeration.Bacteriagrowth ?

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JournalofHarbinInstituteofTechnology(NewSeries),l3,No.6,2006

    quantityunderdifferentsubstrateispresentedin Fig.2.

    

    

    

    

    

    

    '

    

    ?

    FeMn

    substrat

    Aeration

    Fe+Mn

    ?No.aeration

    Fig.2Bacteriagrowthquantityunderdifferentsubstrate ItisshownfromFig.2.thatthebacteriagrew fastandfavorableinthesubstrateonlyincludingFe?.

    ThebacteriacoulddirectlyoxidizeFeandgotthe necessaryenergyofgrowthandpropagationfromitsox

    idationcourse.FromFig.2.itcanbeseenthatthe

    bacteriacouldnotgrowinthesubstrateonlyincluding Mn".Thatmeansthebacteriacouldnotdirectlvmake useofbivalentmanganeseandoxidizemanganese.A1 soitcanbeseenthatinthemixedsubstrateincluding ironandmanganesethebacteriagrewfasterandmore favorablethaninthesubstrateonlyincludingFeh.It meansthattheoxidationofthebacteriaonMnmay beacooperativereactionofoxidizingFe".moreover thebacteriacouldobtainalittleenergyfromthereac. tion.Finallyitisnotedthatbacteriabiomasswasa1. mostnodifierencebetweenaerationandnoaerati0n culture.Whendissolvedoxygenconcentrationwas morethan2.8mg/L(thedissolvedoxygenconcentra. tioninthesubstratebeforeaeration),theincreaseof dissolvedoxgenconcentrationdidnotaffectthebacteri algrowthandpropagation.Thatistosay,

    somelevel

    ofdissolvedoxygenisnonrestrictiveecologicalfact0r0f bacteriagrowthandpropagation.

    2.J.2Therelationofbacteriabiomassandtheron andmanganeseconcentrationvariationofsub. strate

    Accordingtothe5%inoculationquantity.

    the

    bacterialiquorwasinoculatedintothesubstrateinclu. dingbothironandmanganese.TheFe]c(1ncentra.

    tionofthesubstratewas22.06mg/L,theMn]

    concentrationwasl8.67me,/L.Theculturetempera. turewasroomtemperature.Thesamplesweretested

forbacteriabiomass,Fe1concentmtiofland

    [Mn"]concentrationeveryday.Theresultsareshown inFig.3.

    FromFig.3,itisseenthatbacteriabiomassvari. ationpresentedacourseofgrowthcurve.Atthefirst twodays,bacteriawereinthedelayperiodandtheir biomasswasnotobviouslyvariable.Alsoitisn0ted -

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    thatthedeclineoftheironconcentrationofthesub. stratewasrapidandstable.Whenbacteriabiomass wasupto10magnitude,itshowedanuppereffecton manganeseremova1.Butinthefollowingday,therate ofbacterialgrowthbegantoincreasegradually, which

    representedasstrongcellularactivity,fastpropagation rateandthetraitoflogarithmgrowthperiodofrapid bacteriainerement.Bacteriabiomasswasuptothe maximuminthefourthday.Inthefollowingthree days,becausenutrientmatterwasenoughandmetabo. 1izableproductdidnothaveexcessiveaccumulation. thelogarithmgrowthperiodofbacteriawassustained, atthesametimetheironandmanganeseofthesub. stratewerelargelyconsumedandtheirconcentration wasrapidlydeclined.Thisobservationsuggestedthati. ronwhichwastheessentialmetabolicsubstrateofbac. teriaprovidedthebulkofenergyforthebacterial growthandpropagation.Whenbacteriahadthehigher activityandreachedthedefiniteamount.itcouldex. pressasortofactiononthemanganeseconsume.

    -一—?一Mn—?一1)a{?trianumbPr

    Fig?3ConnectionbetweenBacteriagrowthquantityand FeandMnremoral

    2.2ThePurificationEffectOfIBAC

    22.1IronremovalCketofIBAC

    Theresultofironremovalissummm-izedinFig. 4.ItisnotedfromFig.4thatthesystemwhichwasin thestableoperationhadbettereffectontheironremov. alandtheaverageeffluentconcentrationofironwas lessthan0.03mg/L.whichachievesdrinkingwater qualitystandards.AlsofromFig.4.itisobservedthat theironremovalefficiencywasabout98%andthe maximalvaluewasupto99%.Theironremovaleffect requiredwasachievedafter5daysoperation.Thisob. servationsuggestedthatIBACobtainedthemaximal bacterialbiomassofironremovalduringthe5.day0p. erationandthenthesystemwasinthestableoperation state,inwhichtheengineeringbacterialbiomasswas basicallystable.Atcomparatively1owerinfluentc0n centrationofiron,theremovalefficiencyofironwasa. bout97%:atcomparativelyhigherinfluentcoflcefltra. tionofiron,theenzymereactionratecorrespondingly increasedandtheremovalefficiencyofir0nwasstill high.

    2.2.2ManganeseremovalCf~etofIBAC

    上吕【JJ?

    \置毫量蓝

JournalofHarbinInstituteofTechnology(NewSeries),Vo1.13,No.6,2006

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