DOC

Sequence of all oligonucleotides used in study

By Carrie Henderson,2014-11-16 15:56
9 views 0
Sequence of all oligonucleotides used in studyof,in,In,all,used,study,All,Study

1. Oligonucleotides for plasmid construction

     rpGSO146 pET21b-hfq (amp)

    #731 5’-TAT ACA TAT GGC TAA GGG GCA ATC TTT ACA AG #680 5’-CCA AGC TTA TTC GGT TTC TTC GCT GTC C

     rpGSO147 pSP64-T7/oxyS (amp) linearized with HindIII G102C

    #819 5’-CTT TTG CGG ATC TCC ACG ATC CGC AAG CTT GTA #820 5’-TAC AAG CTT GCG GAT CGT GGA GAT CCG CAA AAG

     rpGSO136 pSP64- T7/oxyS (amp) linearized with HindIII 64;109

    #691 5'-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGA ATA ACT AAA GCC AA #690 5'-TAC AAG CTT GCG GAT CCT GGA GAT CCG CAA AAG TT

     rpGSO137 pSP64- T7/spf (amp) linearized with HindIII

    #786 5'-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGT AGG GTA CAG AGG TAA G #787 5'-TAC AAG CTT TAA AAA ACG CCC CAG TC

     rpGSO138 pSP64- T7/fhlA (amp) linearized with HindIII -107;+96

    #790 5’-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGA CCA CAC GCC GAA CCG C #791 5'-TAC AAG CTT CTC ACA CAG CGA GGC C

     rpGSO148 pSP64- T7/fhlA (amp) linearized with HindIII C-13G

    #821 5’-GCT TTG CGG TGC TTT CGT GGA AGA ACA AAA TGT #822 5’-ACA TTT TGT TCT TCC ACG AAA GCA CCG CAA AGC

     rrpGSO115 pCR2.1-T3/6S (amp, kan) linearized with SmaI

    T3/6S(5'-3) 5'- GCC AAG CTC GAA ATT AAC CCT CAC TAA AGG GAT TTC TCT GAG ATG TTC GC

    6S(3'-3) 5'- CGC CCG GGA ATC TCC GAG ATG CCG CC

     rrpGSO113 pCR2.1-T3/5S (amp, kan) linearized with DraI

    T3/5S(5'-1) 5'-GCC AAG CTC GAA ATT AAC CCT CAC TAA AGG GTG CCT GGC GGC CGT AGC GCG

    5S(3'-1) 5'-CCC CGG GTT TAA ATG CCT GGC AGT TCC CTA CTC)

     rrpGSO139 pCR2.1-T3/oxyS (amp, kan) linearized with HindIII

    #797 5'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC #690 5'-TAC AAG CTT GCG GAT CCT GGA GAT CCG CAA AAG TT

     rrpGSO140 pCR2.1-T3/oxyS (amp, kan) linearized with DraI 1;90

    #797 5'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC #800 5'-GCT TTA AAA GTT CAC GTT GGC TTT AG

     rrpGSO141 pCR2.1-T3/oxyS (amp, kan) linearized with SspI 1;69

    #797 5'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC #799 5'-GCA ATA TTA TTC GAG TTG AGA AAC AC

     rrpGSO142 pCR2.1-T3/oxyS (amp, kan) linearized with HindIII 48;109

    #798 5'-CGA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC G #690 5'-TAC AAG CTT GCG GAT CCT GGA GAT CCG CAA AAG TT

     rrpGSO143 pCR2.1-T3/oxyS (amp, kan) linearized with DraI 48;90

    #798 5'-CGA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC G #800 5'-GCT TTA AAA GTT CAC GTT GGC TTT AG

     rpGSO144 pGEM2-T3/oxyS (amp) linearized with SspI 48;69

    #801 5’-AAT TCA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC GAA TAA TAT TA

    #802 5’-AGC TTA ATA TTA TTC GAG TTG AGA AAC TCT CCT TTA GTG AGG GTT AAT TG

     rpGSO145 pGEM2-T3/oxyS (amp) linearized with DraI 64;90

    #803 5’-AAT TCA ATT AAC CCT CAC TAA AGG AAT AAC TAA AGC CAA CGT GAA CTT TTA A

    #804 5’-AGC TTT AAA AGT TCA CGT TGG CTT TAG TTA TTC CTT TAG TGA GGG TTA ATT G

2. Oligonucleotides for oligo blocking analysis

anti-OxyS 5’-AAG AGG TGC CGC TCC GTT TC 1-20

anti-OxyS 5’-GGG TTA AAA GAG GTG CCG CTC C 6-27

anti-OxyS 5’-GCA GTG ACT TCA AGG GTT AA 20-40

anti-OxyS 5’-AGT TAT TCG AGT TGA GAA AC 52-71

anti-OxyS 5’-CAC GTT GGC TTT AGT TAT TC 64-83

anti-OxyS 5’-GCA AAA GTT CAC GTT GG 76-92

3. Oligonucleotides for primer extension assay

OxyS RNA

    #188 5’-GCA AAA GTT CAC GTT GG

fhlA mRNA

    #153 5’-TGT CCG AGA TCA CTC ATC GGT GTA TAT GAC

Spot42 RNA

    #823 5’-CAG CCA AAT CCG ATT ACG

Report this document

For any questions or suggestions please email
cust-service@docsford.com