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Immunological separation and immortalization induction of rat precartilaginous stem cells

By Ryan Butler,2014-06-02 02:22
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Immunological separation and immortalization induction of rat precartilaginous stem cellsof,and,rat,Rat,AND

    Immunological separation and

    immortalization induction of rat

    precartilaginous stem cells ChinaMedicalAbstracts(Surgery),2007,16(2) ShandongProvinHosp,Jinan250021)…?ChinJExp

    Surg.2007,24(2).229--231

    ObjectiveToobservetheeffectofherpessimplex virus-thymidineldnase/cytosinedeamin&sefusionsuicide geneontheosteosaroomagrowthofnudemice.Methods Osteosarcomamodelofnudemousewasmadewith MG-63cellsinjectedintoaxillaryfossa.Fortyeightnude

    miceweredividedrandomlyinto6groups:(1)PBScon

    trolgroup;(2)Ad-LacZ/GCV+5FCgroup;(3)Ad-

    TK/GCVgroup;(4)Ad-CD/5F?group;(5)AdTK/

    GCV+Ad-CD/5FCgroup;(6)AdTKCD/GCI,,+5

    FCgroup.Tumorvolume,inhibitoryrateoftumorgrowth andhistopathologywereanalyzedaftereachgroupwas treatedwithgeneandprodrug.ResultsTherewasno significantdifferenceintumorvolumeandrateoftumor celldeathbetweenAd-LacZ/GCV+5FCgroupandPBS

    controlgroup.TumorgrowthinTK,groupAd-

    CD/5FCgroup.AdTK/GCV+AdCD/5FCgroupand

    Ad-TK-CD,FCgroupwasinhibitedsignificantly

    ascomparedwithPBScontrolgroupandA_d..Lac-.Z/GCV +5FCgroup(P<0.05).Therewassignificantdiffer

    encebetweenAd-TK/GCV+AdCD/5FCgroup.Ad_

TK-CD/GCV+5FCandAD-TK/GCVgroup.Ad-CD/

    5-FCgroup(P<0.05).ConclusionHSVTK/CDfu

    sionsuicidegenecouldsignificantlyinhibittheosteosarco

    maofnudenaoHse.11refs,1tab.

    (Authors)

    3.7Others

    207245Immunologicalseparationandimmortalization inductionofratprecartilaginousstemcells/GuoFengjin (郭风劲,DeptOrthop,TongjiHasp,ToliMedCol,

    HuazhongUnivSciTechnolWuhan430030,f}ChinJ

    ExpSurg.2007,24(2).226--228

    ObjectiveTodiscussthemethodofseparatingand purifyingprecartilaginousstemcells(PSi2)byimmuno

    magnetictechnology,andestablishimmortalizedPSC(IP

    SC)strainandprovidestablecel1resourceforcelltrans

    plantationandgenetherapieswithPSC.6refs,3figs. MethodsImmunomagneticseparationwasusedtoisolate PSCslabeledwithfibroblastgrowthfactorreceptor-3 (FI矾一3).PlasmidpEG-FPIRES2SV40containingthe

    simianvirus40largeTantigengene(SV40Tag)was transfectedintotheprimarilyculturedPSCsofnewborn ratusinglipofectintransfectionmethod.Colonieswereiso

    latedbyG418selectionandexpandedtoimmortalizedcell strain.AntiFGFR3,collagentype1IandtypeXanti

    bodieswereusedtoidentifytheculturedcellsandtoinves

    tigatethecapabilityofdifferentiationofthetransfected cellseexpressionofSV40Taginexpandedcelllines wasdetectedbyRT_PCR,Southernblotand~munocyto

    chemistry.ResultsOneantiG418cellclonewasob

talned.whichwasconfirmedasFGFR3positivePSi2with

    thecapabilityofproliferation.eexpressionofSV40Tag mRNAandproteinwasdetectableintransfectedcellsafter stabletransfection.Thetransfectedcellswereexpandedto immortalizedcel1strainmaintainedformorethan50pas

    sages,namedasIPSC.IPSCswereellipticortriangular cellswithtwoorthreeshortaxons.Thepopulationdou

    blingtimeofIPSCwas23.62h.Subculture,freezingand recoveringhadnoeffectoncellularshapeandproliferation ofIPS~2.ConclusionTransfectingSV40Tagcouldim

    mortalizePSC.TheestablishmentofFGFR3positiveIP

    SCstrainmayprovidestablecellresourceforthebasicre

    searchesandcelltransplantationtherapieswithPSi2.6 refs,3figs.

    (Authors)

    207246Chondrogeniedifferentiationofrabbitbone marrow-derivedmesenehymalstemcellsinvitrobyag- gregateeulture/Du~Xiaojull(段小军,GentreJoint

    Surg,SouthwestHosp,3rdMi1MedUniv,Chongqmg 400038)…?ChinJExpSurg.2007,24(3).316

    318

    ObjectiveToinvestigatethepotentialofapplication ofaculturesystemthatfacilitatesthechondrogenicdiffer

    entiationofrabbitbonemarrow-derivedmesenchymal stemcells(MSCs).MethodsCellsobtainedinbonemar

    rowaspirateswereisolatedbymonolayerculturefrom4 rabbitsandtransferredintotubesandallowedtoform three?dimensionalaggregatesinachemicallydefinedmedi..

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