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Water Potential problem set

By Greg Sanders,2014-06-23 10:37
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Water Potential problem set

Ms.Sastry Unit 2/Chp 8 1

    Water Potential problem set 1 and 2 :

Use text book page Pg 752 to help you.

Show your work:

    1. A solution in a beaker has sucrose dissolved in water with a solute potential of -0.5MPa. A flaccid cell is placed in the above beaker with a solute potential of -0.9MPa. a) What is the pressure potential of the flaccid cell before it was placed in the beaker?

    b) What is the water potential of the cell before it was placed in the beaker?

c) What is the water potential in the beaker containing the sucrose?

d) How will the water move?

    e) What is the pressure potential of the plant cell when it is in equilibrium with the sucrose solution outside? Also, what is its final water potential when it is in equilibrium?

f) Is the cell now turgid/flaccid/plasmolysed?

g) Is the cell hypotonic or hypertonic with respect to the outside?

h) If it is hypo/hyper (choose one) tonic this means that its water potential is

    higher/lower (choose one) than the outside.

    2. A solution in a beaker has sucrose dissolved in water with a solute potential of -0.7MPa. A flaccid cell is placed in the above beaker with a solute potential of -0.3MPa. a) What is the pressure potential of the flaccid cell before it was placed in the beaker?

    b) What is the water potential of the cell before it was placed in the beaker?

c) What is the water potential in the beaker containing the sucrose?

d) How will the water move?

Ms.Sastry Unit 2/Chp 8 2

    e) What is the pressure potential of the plant cell when it is in equilibrium with the

    sucrose solution outside? Think carefully does the plant cell wall change shape?

f) Also, what is the cell’s final water potential when it is in equilibrium?

g) Is the cell now turgid/flaccid/plasmolysed?

h) What is the cell’s solute potential when it is in equilibrium?

    i) Is the cell hypotonic or hypertonic with respect to the outside?

    j) If it is hypo/hyper (choose one) tonic this means that its water potential is

    higher/lower (choose one) than the outside.

Still confused do the Case study for Chp 36 it is a good one!

Ms.Sastry Unit 2/Chp 8 3

    Old problems: you already did these! Just in case… you did not…

    You are given this:

    Solute potential (ψs) = iCRT

    i = The number of particles the molecule will make in water; for NaCl this would be 2; for sucrose or glucose, this number is 1

    C = Molar concentration (from your experimental data/problem)

    R = Pressure constant = 0.0831 liter bar/mole K

    T = Temperature in degrees Kelvin = 273 + ?C of solution

    1. Calculate the water potential of a solution of 0.15 M sucrose (assume for all problems that RT = 2.478).

     2. If a flaccid cell (ψp = 0) having a solute potential of -0.65 MPa is placed in the above solution, what will be its pressure potential at equilibrium?

     3. If the cell above is removed from that solution of 0.15 M sucrose and placed in a solution of 0.35 M sucrose, will the pressure potential of the cell increase or decrease? What will be the new value?

     4. You measure the total water potential of a cell and find it to be -0.24 MPa. If the pressure potential of the same cell is 0.46 MPa, what is the solute potential of that cell?

     5. If a cell having a solute potential of -0.35 MPa is placed in a solution of pure water, what will be its pressure potential at equilibrium?

     6. What is the water potential of a cell with a solute potential of -0.67 MPa and a pressure potential of 0.43 MPa?

7) A cell is in equilibrium with an outside solution where Ψoutside = -1.0 MPa. Water

    is added to the outside solution such that Ψoutside = -0.2 MPa and the cell volume

Ms.Sastry Unit 2/Chp 8 4

    increases 1.5 times. What Pressure potential is required to stop the movement of

    water into the cell?

8) A hypertonic environment has a High/Low (circle one) water potential compared to

    the cell? Why?

    9) Then, water will move which way according to water potential rules?

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