Standard Operating Procedure
for work with
Chemical name/class: Acrylamide (Customization required - CAS #: 79-06-1
Building: Room #:
Designated Work Area:
1. Circumstances of Use:
Acrylamide is commonly used in labs for polyacrylamide gel electrophoresis and to aid in DNA precipitation.
Please include circumstances of use (if different than above), along with concentration and quantity.
2. Potential Hazards:
; Acrylamide is considered a Particularly Hazardous Substance because it is a probable human carcinogen.
; Possible routes of exposure include inhalation, skin contact, eye contact, and ingestion. Acrylamide is highly
toxic by inhalation and skin contact. It can easily penetrate intact skin.
; Acrylamide is known to affect the nervous system with early signs of exposure including numbness, tingling, and
tenderness to touch. Symptoms can be delayed several days to weeks and if exposure continues (even in small
quantities), other symptoms may arise including excessive sweating, blue-reddish skin, peeling of skin, and
weakness in limbs.
; Animal studies have shown some maternal and paternal reproductive health effects from exposure to
; Acrylamide may cause sensitization by inhalation or contact with skin.
; Acrylamide may polymerize explosively if heated to 183?F (84?C).
; Consult the Laboratory Chemical Safety Summary for acrylamide for additional information on hazards.
3The OSHA Permissible Exposure Limit for acrylamide is 0.3 mg/m. The American Conference of Governmental 3Industrial Hygienists (ACGIH) has a Threshold Limit Value of 0.03 mg/m.
3. Engineering Controls:
If aerosols may be produced (e.g., weighing powder), acrylamide and any suspensions of acrylamide must be
handled in a chemical fume hood, exhausted biological safety cabinet with negative pressure ductwork, or other
exhausted enclosure. Aerosols may be produced during any open handling of dry powder, and during open or pressurized manipulations of suspensions. It is recommended that labs post this information sheet on Working Safely with Toxic Powders.
4. Work Practice Controls:
; Laboratory-specific written procedures are required for acrylamide, including a designated work area (at top of
; When possible, order pre-cast polyacrylamide gels to avoid work with acrylamide powder. Acrylamide can also
be ordered already in solution.
; Avoid contact with incompatibles including metals, oxidizing agents, reducing agents, acids, bases, and
; Use bench pads to cover areas that may become contaminated with acrylamide powder or suspensions for easy
; Wipe down the surfaces where acrylamide is used periodically with a detergent and water solution. To
decontaminate surfaces, use a 1.6% potassium persulfate solution followed by 1.6% sodium metabisulfite. Let
stand for 30 minutes, then wash/wipe with plenty of water.
; If weighing dry acrylamide powder and the balance cannot be located in a fume hood or BSC, tare a container
then add the material to the container in a hood and seal the container before returning to the balance to weigh
; Change gloves regularly (at least every two hours) and wash hands at the time of the glove change.
; Keep containers closed as much as possible.
; Keep away from heat and flame.
5. Personal protective equipment (PPE):
; Standard nitrile laboratory gloves and a fully buttoned lab coat with sleeves extending to the wrists should be
worn when handling acrylamide. When handling suspensions or solutions, choose a glove that is protective
against the solvent. If gloves are splashed or come in contact with acrylamide, change them as soon as possible.
; If splashes may occur, wear goggles. Otherwise, wear standard laboratory safety glasses.
; In cases where the arms or torso may be exposed to liquid suspensions or dry particles, wear Tyvek sleeves
and/or gowns (or other air-tight non-woven textile).
6. Transportation and Storage:
; Acrylamide must be in sealed shatter-resistant containers during transportation. If the container is not shatter-
resistant, use a secondary container.
; Store away from heat and flame.
; Store acrylamide away from any incompatible materials including metals, oxidizing agents, reducing agents,
acids, bases, and peroxides.
7. Waste Disposal:
Unwanted acrylamide (and suspensions) must be disposed of following your laboratory-specific chemical hygiene
plan, Duke University’s Chemical Waste Policy and the Laboratory Chemical Waste Management Practices.
8. Exposures/Unintended contact:
Contact Employee Occupational Health and Wellness (EOHW) at 919-684-3136 for medical advice on occupational chemical exposures. For an actual chemical exposure
; Remove contaminated clothing. Flush exposed eyes or skin with water for at least 15 minutes, then seek
; For situations with risk of inhalation exposure (including spills of powder outside of a chemical fume hood),
remove all persons from the contaminated area and contact the OESO spill team.
; Call 911 from a campus phone or 919-684-2444 from any phone to request assistance if needed. Contact
Employee Occupational Health and Wellness at 919-684-8115 for exposure-related advice.
The work-related injury or illness report found at: http://www.hr.duke.edu/benefits/medical/workcomp/report.php
should be completed within 24 hours. Follow-up medical attention should be sought through Duke Employee Occupational Health and Wellness (919-684-3136).
9. Spill Procedure:
; Spills of dry acrylamide powder outside of a chemical fume hood or other enclosure should be referred to the
OESO spill response team by calling 911 from a campus phone or 919-684-2444 from any phone.
; Spills of liquid suspensions should be absorbed with sand or other non-combustible absorbent material and
placed in containers for disposal. Decontaminate the area with 1.6% potassium persulfate, followed by 1.6%
sodium metabisulfite. Rope off the area and let stand for 30 minutes, then wash/wipe area with plenty of
; For spills of dry powders in a chemical fume hood or other enclosure, wipe up the powder using a cloth
dampened with water, or wet the powder with water and then wipe with a dry cloth. Minimize the fume hood
or enclosure opening during this process. Decontaminate the area with the solutions mentioned in the previous
bullet. Place any clean-up materials into a bag and seal, then submit as hazardous chemical waste (see waste
disposal section above) through OESO Environmental Programs (call 919-684-2794 with questions).
10. Training of personnel:
; All personnel are required to complete the online General Lab Safety session thru the OESO website. This
session includes an introduction to general chemical safety.
; Training on the specific procedures is required for all personnel working with these materials, and must be
documented (topics covered, date, employee names and signatures).
; All personnel shall read and fully adhere to the laboratory-specific SOP for toxic powders, and shall document
that they have read it by signing and dating the SOP.
“I have read and understand this SOP. I agree to fully adhere to its requirements.”
Last First Duke ID Signature Date