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myc epitope sequence EQKLISEEDL

By Gilbert Thompson,2014-04-08 20:54
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myc epitope sequence EQKLISEEDL

myc epitope sequence: EQKLISEEDL

    Reverse translate, E. coli organism bias:

     E Q K L I S E E D L GAA CAG AAA CTG ATC TCT GAA GAA GAC CTG CTT GTC TTT GAC TAG AGA CTT CTT CTG GAC

Fill Top BamHI overhang:

Avoid regenerating BamHI restriction site when filling in

    the overhang.

5' gag gat ctg

    3' ctc cta gac

     E D L

Leucine chosen as third amino acid because of hydrophobic

    quality, which is similar to the proline that used to be

    there.

Introduce silent EcoRV restriction site:

     E Q K L I S E E D L GAA CAG AAA CTG AT|A TCT GAA GAA GAC CTG CTT GTC TTT GAC TA|T AGA CTT CTT CTG GAC

Final Top Strand:

    gat cTG GAA CAG AAA CTG ATA TCT GAA GAA GAC CTG

Complementary Bottom Strand Added:

    gat cTG GAA CAG AAA CTG ATA TCT GAA GAA GAC CTG

     AC CTT GTC TTT GAC TAT AGA CTT CTT CTG GAC cta g

Elminate Regenerated BamHI restriction site:

gat cTG GAA CAG AAA CTG ATA TCT GAA GAA GAC CTA

     AC CTT GTC TTT GAC TAT AGA CTT CTT CTG GAT cta g

Final Bottom Strand:

    AC CTT GTC TTT GAC TAT AGA CTT CTT CTG GAT cta g

Final Strand:

gat cTG GAA CAG AAA CTG ATA TCT GAA GAA GAC CTA

AC CTT GTC TTT GAC TAT AGA CTT CTT CTG GAT cta g

D L E Q K L I S E E D L D

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