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to-be-conditioned

By Henry Sanchez,2014-03-27 11:46
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to-be-conditioned

SUPPLEMENTARY DATA

    Supplementary Figure 1. Presence of abnormally enhanced latent inhibition (LI) following late prenatal immune challenge. Pregnant C57BL/6 mice on gestation day 17 were treated with the viral mimic Poly-I:C (5 mg/kg, i.v.) or corresponding control vehicle (sterile pyrogen-free 0.9% NaCl) solution as fully described in the Supplementary Materials and Methods. The resulting male offspring were subjected to

    LI testing when they reached the adult stage of development (postnatal day 90). The LI effect was studied in a two-way active avoidance procedure, in which an 80dB white noise stimulus served as the A

    to-be-conditioned stimulus (CS), and electric foot shock (0.3 mA) served as the unconditioned stimulus (US) (see Supplementary Materials and Methods for a detailed description of the test apparatus and

    procedures used). Pre-exposed (PE) subjects were presented with 40 CS-pre-exposures prior to conditioning, whereas non-pre-exposed (NPE) subjects were not pre-exposed to the CS. (a) The line plots

    depict the number of avoidance shuttles (square-root [sqrt] transformed) as a function of blocks of 10

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    trials, and the bar plots depict the mean number of avoidance shuttles (square-root [sqrt] transformed) per block performed during the CS-US conditioning phase of the test. CS-pre-exposures did not induce a LI effect (i.e., a decrease in conditioned responding following repeated CS-pre-exposures prior to conditioning) in offspring born to control mothers. However, a marked LI effect was present in male Poly-I:C offspring, as evident by the reduction in the conditioned avoidance responses performed by PE Poly-I:C subjects relative to NPE Poly-I:C subjects. *P < 0.05, signifying the significant main effect of pre-

    exposure in corresponding one-way ANOVAs performed at individual 10-trials blocks restricted to Poly-I:C offspring; restricted ANOVAs were performed following presence of a significant three-way interaction in the initial 2 × 2 × 10 (prenatal treatment × pre-exposure × 10-trials block) ANOVA of

    conditioned avoidance shuttles [F(9,180) = 2.06, P < 0.05]. (b) The line plots depict the response latency

    (square-root [sqrt] transformed sec) as a function of blocks of 10 trials, and the bar plots depict the mean response latency (square-root [sqrt] transformed sec) per block performed during the CS-US conditioning phase of the test. Consistent with the outcomes in the analysis of avoidance shuttles, pre-exposures to the CS prior to conditioning did not induce a LI effect in offspring born to control mothers. Hence, the response latency between control NPE and PE subjects were highly comparable. In contrast, a significant LI effect (i.e., a decrease in response latency following repeated CS-pre-exposures prior to conditioning) was present in male Poly-I:C offspring. *P < 0.05, signifying the significant main effect of

    pre-exposure in the 2 × 10 (pre-exposure × 10-trials block) ANOVA of response latency restricted to Poly-

    I:C offspring [F(1,10) = 4.04, P < 0.05]; restricted ANOVAs were performed following presence of a

    significant interaction between prenatal treatment and pre-exposure [F(1,20) = 4.83, P < 0.05] in the

    initial 2 × 2 × 10 (prenatal treatment × pre-exposure × 10-trials block) ANOVA of response latency. The

    number of subjects in each prenatal treatment group and pre-exposure condition was 6 males. All values are means ? SEM.

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