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qiagen plasmid buffer recipe

By Clifford Ferguson,2015-03-23 23:42
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Note: Buffer compositions are given per liter of solution. Do not autoclave isopropanol-containing buffer, but sterilize by filtration instead. Buffer calculations are base on Tris base adjusted to pH with HCl (Tris-Cl). If using Tris-HCl reagent, the qualities used should be recalculated. Buffer P1 - Resuspension Buffer 50mM Tris-Cl, pH 8.0, 10mM EDTA, 100ug/mL RNa..
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