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stimulated bioremediation in phenol-contaminated groundwater

By Clarence Clark,2014-02-17 20:22
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stimulated bioremediation in phenol-contaminated groundwater

    stimulated bioremediation in

    phenol-contaminated groundwater

    MTBEbiodegradatiOnanddegradermicrobialcommunitydynamicsin

    MTBE}BTEXfandheavymetalcontaminatedwater

    LinCW,LinHC,LaiCY

    DaYehUniv,DeptEnvironmEngn,Changhua515003,Taiwan

    NatlChanghuaUnivEduc,DeptBiol,Changhua515,~iwan

    LinCW(reprintauthor).6aYehUniv,DeptEnvironmEngn,ll2ShanJiauRd,Changhua515003,Taiwancwlin@mail.dyu.edutw

    Abstract:Theaimofthisinvestigationwastoexploremicrobialcormnunitychangesundervariousenvironmental

    groundwaterconditions(singlesubstrate,mixedsubstrates,andthepresenceofheavymetals)andlinkthechanges

    withsimultaneouslydiminishingsubstrateconcentrationinthemicrocosms.Mostmicroorganismsfromenviron

    mentalmicrocosmsorwastewatertreatmentplantscannotbecultivatedartificially.CapturingmicrobialCOlnnmnity

    fingerprints,therefore,requiresapplyingamolecularbiologicaltechnique.ByusingSSCPprofilesofPCRamplified

    16SrDNAgenes,itwasdemonstratedthatwiththerepeatedadditionofsubstratesduringlongtermacclimation,

    substrateutilizingpopulationsinamicrocosmgraduallyincreasedtobecomethedominantconstituent.Conversely,

    thepresenceofmetalsinhibitedcommunitydevelopmentanddifferentiation.Itwasalsoshownthatsubstrate

    degradationratesincreasedundercosubstrateconditions,withsubstrate

    degraderseasilyadaptingtotheenviron

    mentandbecomingthedominantbacteria,aphenomenonattributedtothepropensityofthefittestspeciesto

    outgrowtheircompetitorswhenpresentedwithsuitablesubstrates.

    Keywords:16SrDNA:metalions;microbialcommunitystructure:molecularprofiling;methyltertbutylether

    TEr-BUIYLETHER:STRANDCONFOIArIONPOL,MORPHISM:BACTERIAI,COM

    MUNITIES;GENETIC

    PROFILES;SOIL;PCR:DEGRADA

    ON:PM1;HYDR0CARBONS;GR0UNDWATER

    INTERNATIONALBIODETERIORATION&BIODEGRADATION2007,59(2):9

    7102

    Microbialcommunitystructureduringoxygenstimulatedbioremediation

    inphenolcontaminatedgroundwater

    LinCW,LaiCY,ChenLH,ChiangWF

    DaYehUniv,DeptEnvironmEngn,Changhua5l5,Taiwan

    NatlChanghuaUnivEduc,DeptBiol,Changhua,Taiwan

    TaichungCtyBurEnvironmProtect,Taichung,Taiwan

    LinCW(reprintauthor),DaYehUniv,DeptEnvironmEngn,112ShahJiauRd,Changhua5l5,Taiwancwlin@mail.dyu.edutw

    Abstract:Thisresearchexploredthechangesingeneticdiversityandspatialdistributionofmicrobialcommunities

    inassociationwiththechangesinphenolconcentrationduringabioremediationprocess.Resultsusingthetradi

    tionalplatecountmethodindicatedallincreaseofaveragebacteriadensitiesingroundwaterfrom10(4)to10(7)

    CFU1111(1)initiallyto10(7)to109CFUml(

    1)afterremediation.Thediversityandstabilityofphenoldegrading

    bacterialcommunitieswereinvestigatedbyusingsinglestrand

    conformationpolymorphism(SSCP)geneticprofile

    analysisof16SrDNAfragmentsamplifiedfromgroundwatersamples.Themoleculardatash

owedahighdegreeof

    .

    geneticsimilaritybetweencommunitiesfromcertainmonitoringwellsduringtheearlyphasesofremediation,prob

    ablyduetosimilarinitialphysicalconditionsamongwells.Molecularsignaturesofseveralcultivatedpheno1degrad

    ingbacterialstrainscouldbeseeninmostgronndwaterprofilesthroughoutthestudyperiod,suggestingthatthese

    strainswereindigenoustothestudysite.Itwasalsoobservedthatthespeciesdiversityofthesemicrobia1COmlTIu.

    nitiesjncreasedasthephenol

    evelsjnthegroundwaterdecreasedduringthe9.monthstudyperiod.andrecovered tothepre.treatment1evelsaftertheremediationproamwascompleted.

    Keywords:16SrDNA:groundwater;microbia1community;phenol;remediation;SSCP STRAND.CONF0RMATIONP0L'YrM0RPHISM:BACTERCOMMUNITrES:AROM

    ArIC.HYDR0CARBONS:

    S0IL;BIODEGRADATION:AOUIFER

    JOURNALOFHAZARDOUSMATERIALS2007,14o(12):221-229

    Planktonicmicrobialcommunitiesassociatedwithfracture.derived groundwaterinadeepgoldmineofSouthAfrica

    LinLH,HallJ,OnstottTC,GihringT,LollarBS,BoiceE,PrattL,

    LippmannPipkeJ.BellamyRES

    PrincetonUniv,DeptGeosci,Princeton,NJ08540USA

    PacificNWNat1Lab,Washington,DCUSA

    UnivToronto,DeptGeol,Toronto,ONCanada

    IndianaUniv,DeptGeo1Sci,Bloomington,IN47405USA

    GeoforschungszentrumPotsdam,Tele~afenberg,D14473Potsdam,Germany

    LinLH(reprintauthor),NatlTaiwanUniv,DeptGeosci,1,Sec4,RooseveltRd,TaipeiJ06,Taiwanlhlin@ntu.edu.tw

    Abstract:Thevertica1distributionandfunctionofterrestria1planktonicmicrobia1communitiesatdepthsgreater

    than600mremainpoorlyestablished.Culture.independentmethodsusing16SrRNAgenesandgeochemicalaD.

    proacheswereemployedtoinvestigatetheheterogeneityandpotentialfunctionofmicrobia1communitiesresiding

    withinfracturesat0.7to1.4kilometersbelow1andsurfaceofBeatrixAuMine.SouthAfrica.Thesalinity(26to47

    mMC1.),temperature(33to40degreesOandagef1to5Ma)ofthesefracturewaterjncreasedwithdepth.The

    deltaDanddeltaO.18valuesoffracturewaterrangedfrom.44to.39%andfrom.7to.4%VSM0Wrespectively,

    andexhibitedamixingtrendwithfracturewatercollectedfromthesamemineinapreviousstudywhereisotopic

    signatureswerejndicativeofhydrotherrnalorigin.FracturewaterfromBeatrixMinewasdistinctfrO1/1theground.

    waterintheoverlyingKaroosedimentarystrataifltermsofitsC1.,HeandCH4cOncentratiOns.anditsdeltaDand

    deltaO.18sigmaturesandfromVaa1River(sourceofservicewater1intermsofitsdeltaDanddeltaO.18signatures.

    Thedifferencesconstrainthemaximumamountofmixingwithservicewaterorshallowgroundwatertobe1ess

    than4%.The16SrDNAanalysesrevealeddiverseandnmnerousnove116SrRNAgenesaffiliatedwithProteobacteria.

    Firmicutes,Nitrospira,Chlorobi,Thermus,CandidateDivisionOP3andEuryarchaeota.Theproportionofeach

    phyluminclonelibrariesvariedmarkedlyamongsamplesandsuggestskm

    scale,spatialheterogeneityincoFnnqu

    nitystructures.Potentia1metabolismsinfc:rredfromthepresenceof16SrRNAgenesaregenerallyconsistentwith

estimatesoftheavailablefreeenergy.

    Keywords:16SrRNAgenephylogeny;planktoniccommunities;SouthAfricangoldmine;s

    ubsurfaceecosystems

    SP.NOV:WITWATERSRANDBASIN:PHYLOGENETICANA

    SIS:BACTERIALDIVERSITY;SEA

    SEDIMENTS;BETA.SUBCLASS;AFIPIA.FELIS;C0MB.N0V:GEN.NOV:PR0TEOBACTERIA

    GEOMICROBIOLOGYJOURNAL2006,23(6):475-497

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