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Identification

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IdentificationIdenti

    Identification

    June2008JournalofNortheastAgriculturalUniversityVo1.15No.21-4 ;IdentificationofColdToleranceofRiceGermplasmResourceat ;GerminatingStageinColdRegion

    ;LIUHualong,SUNShiche,WANGJingguo,andZOUDetang

    ;CollegeofAgriclture,NortheastAgriculturalUniversity,Harbin150030,China ;HeilongjiangAcademyofAgriculturalSciences,Harbin150086,China ;Abstract:Total75ricevarieties(1ines)inHeilongjiangProvince(orcoldregion)asgermpla

    smresourceswereidentifiedforcold

    ;toleranceatgerminatingstagebycontrollingtemperatureinartificialincubator.Theresults

    showedthattheshootingseedrateatthe

    ;germinatingstagecouldbeusedastheevaluationindexofcoldtolerance.Thecoldtolerance

    wasrecordedon1-9scaleandcouldbe

    ;identifiedbvthecriteriaoffiveindexessuchashighlytolerant(HT),tolerant(T),moderatel

    ytolerant(MT),susceptible(S),highly

    ;susceptible(HS).

    ;Keywords:rice,coldtolerance,germplasmresource,identification ;CLCnumber:$511:$426Documentcode:AArticleID:10068104(2008)

    02000104

    ;Introduction

    ;RiceisoneofthemaincerealcropsinHeilongjiang

    ;Province.Thericegrowthperiodgetsshorterinthe

    ;temperatezoneofthisprovince.Chillinginjuryisone

    ;ofthemostnaturalfaminetoinducetheyielddeclinein

    ;riceproduction.Oneofthemosteffectivewaysofre

    ;sistinglowtemperaturestressisricebreedingoflow

    ;temperaturetolerance.Itisreportedthatacloserela

    ;tionofcoldtoleranceatgerminatingstage,booting

    ;stageandfloweringstageisobserved_lJ,therefore,the ;coldtoleranceatgerminatingstageiswidelyusedin

    ;theidentificationofficegermplasmresource.

    ;Inthispaper,75varieties(1ines)wereidentifiedfor

    ;coldtoleranceatgerminatingstagebycontrollingtem

    ;peratureinartificialincubator.Coldtolerancechar

    ;actersofthesematerialswerediscussedasatheoretica1 ;basisforthericechilling--tolerantbreedinginHeilong-- ;jiangProvince.

    ;MaterialsandMethods

    ;Materials

    ;Total75varieties(1ines)werecollectedbyRiceBreed

    ;ingLabofNortheastAgriculturalUniversityasexperi- ;mentalmaterialswhichdistributedinmostriceregion ;andrepresentedbasicallythemaincategoriesofrice ;varietiesinHeilongjiangProvinceatpresent. ;Methods

    ;Tbtal100fullseedsfromeachmaterialwereputinto ;aculturedishwithfilterpaper.Thenthedisheswere

    50?tomakethe ;keptinincubatorfor48hat45

    ;seedsdryfullyandtheseedsdormancywasbroken. ;Afterseedsoaking,seedsbegantogerminatein ;incubatorat30.C.Whenthebudsreached5113111long, ;thetemperaturewasregulatedinto5~Cfor10days, ;thenthedisheswereputintoaroomofabove20?to

    ;makebudsgrownormallyfor710daysAfterall

    ;Receivedl7December2007

    ;LIUHualong(1978

    ),male,Master,asistantlecturer,engagedintheresearchofricegeneticsbreeding.

    mail:zondt@163.com ;Towhomcorrespondenceshouldbeaddressed.E

    ;http://publish.neau.edu.c[i

    ;

    ;?2?JournalofNortheastAgriculturalUniversityVl-15No.22008

    ;thesesteps,thealiveseedlingswerecountedandthe ;survivalseedlingratewasformulated.Acomplete

    ;lyrandomizedexperimentwascompletedwiththree ;repeats.

    ;Thesurvivalseedlingratesof75materialswere ;evaluatedasaindexofcoldtoleranceatgerminating ;stage.

    ;Thesurvivalrate(%)=(thesurvivalseedlings/the ;totalbuddingseeds)×100%

    ;ResultsandAnalysis

    ;Establishmentofscalesforcoldtoleranceat ;germinatingstage

    ;Thecoldtoleranceatbudstagewasdefinedasthe ;abilityforyoungbudstoremainaliveincoldwater, ;andappearedasanabilityofinductionofgreenseedl

    ;ingfromyoungbud[3_4.

    ;Therefore,thecoldtoleranceatbudstagewaswide

    ;lyappliedincoldtoleranceidentificationofricegerm

    ;plasmresource.

    ;Theevaluationofcoldtoleranceatbudstagewas ;madebywayofsurvivalseedlingrateandrecordedin ;5criteriasof1-9scalesuchashighlytolerant(HT), ;tolerant(T),moderatelytolerant(MT),susceptible(S),

    ;highlysusceptible(HS).Theclassificationcriteriaare ;showninTable1.

    ;Table1Classificationstandardofcoldtoleranceatbudstage ;Differenceofcoldtoleranceatgerminating ;stageamongvarieties

    ;Thecoldtoleranceof75materialsatbudstagewas

    mail:xuebaoenglish@neau.edu.crl ;E

    ;analyzedandshowninTable2,andextremelysigni

    ;ficantdifferencesamongexperimentalmaterialsof ;coldtoleranceatbudstagewerefound.

    ;ThedeadseedlingrateofBinxuwasthelowest ;(6.45%)andthatofJinfu13,Zupei64wasthehigh

    ;est(100%).

    ;Therangeof93.55%fromthelowesttothehighest ;showedtheextremelysignificantdifferencesincold ;toleranceatbudstageamongvarieties.

    ;Thedeadseedlingratesin10varietieswerebetween ;10%30%andthatin24vailetieswerebetween30%

    ;50%andin17varietiesbetween50%90%andin14

    ;varietiesabove90%.

    ;Basedontheclassificationstandardofcoldtolerance ;atbudstage,thehighlytolerantvarieties(HT)with ;90%greenseedlingrateinducedfromyoungbudsand ;withdeepgreenleaveswereBinxu,Xueguang,Tongxi ;120,T0ngyu250,Shangyu397,Dongnong416,Song ;9798,Song938,Shashani,Dongnong9601;thetole

    ;rancevarieties(T)wereJifeng10,Xiaobaidao,Yan ;401,Teyou14,Yan105,Dongnong421,Daobei43, ;Xinxue,Xingjiao503,Dongnong3114;themoderately ;tolerantvarieties(MT)wereSong237,Fengqi1,4227x ;Fushiguang,Pinnuo19,Song9810,Xixuan1,Ken92

    ;509,Hejiao910612;thehighlysusceptiblevarieties ;(}IS)wereSui112,Longxuan948,99F13,Fengyou303,

    ;Dongnong423,Jinfu19,Jinongda3,Jinfu13,Dongnong ;715,Fushiguang,Xingzhimeng,Hejiang23,Hanjiu, ;Zupei64;thesusceptiblevarieties(S)wereTeyou16, ;Fengyou503,Teyou14,Jinfu1,Jinfudao,Dongnong ;911,Nonglin20,Jinfu15,Dongnong418,Fengyou307, ;Teyou21,Dongnong9825,Xuenuo,Caidao,Tongyu

    ;139,Jifeng2605,Ji8611,Dongnong9219,Zhongmu

    ;35,Jinfu20,Changlixiang,Hejiang19,Mu161,Bai

    ;shoushimao.

    ;Experimentalresultsshowedthatthechillinginjury ;reactionofdifferentricevarietiesunderlowtempera

    ;turewasveryobvious,andhadgoodreproducibility. ;Thereforeitispossibletoselectthcstableandreliable

    ;coldtolerancevarietiesbytheidentificationofcold ;toleranceofdifferentricevarietiesatgerminating ;stage.

    ;

    ;LIUHualongela1.IdentificationofColdToleranceofRiceGermplasmResourceatGermi

    natingStageinColdRegion

    ;Table2Differencesofcoldtoleranceatbudstageamongvarieties

    ;N..VarietyDea

    ;mt

    ;d

    ;e

    ;se

    ;r

    ;e

    ;%

    ;d

    ;1

    ;li”gsca1eMarkN..VarietyDea

    ;rat

    ;d

    ;e

    ;se

    ;r

    ;e

    ;%

    ;d

    ;1

    ;lings

    ;ca1eMark

    ;LO1Jifeng1016.583TL39Song23760.647S ;L02Teyou1640.005MTL40Song26966.407S ;L03Fengyou50334.805MTL41Song9388.561HT ;L04Sui11294-359HSL42Teyou1425.503T ;L05Longxuan94890.859HSL43Fengyou30734.205MT ;L0699F1396.029HSL44Fengyou30179.457S ;L07Teyou1444.805MTL45Fuyuan470.027S ;L08Fengyou30399819MSL46Teyou2141.605MT ;L09Jinfu148.505MTL47Dongnong982532.305MT

    ;LIOTongyu2509.521HTL48Teyou1368.277S ;L11Jinfudao49105MTL49Song97987.861HT

    ;L12Jinfu1991-359HSL50Dongnong96018.671HT ;L13Jinongda399509HSL51Xueguang6.581HT

    ;L14Dongnong911943.505MTL52Jingyin5963.657S ;L15Nonglin2034.805MTL53Shangyu3977.951HT ;L16Zupei282.047SL54Yan10518.623T

    ;L17Jinfu13100.009HSL55Dongnong4168.641HT ;L18Jinfu1535.605MTL56Xuenuo34.705MT

    ;L19Dongnong41834.805MTL57Binxu6.451HT ;L20Xingjiao50326.413TL58Caidao45.405MT ;L21Fengyuan60356637SL59Tongyu13948005MT

     ;L22Xiaobaidao16.703TL60Kenxiaodao78.097S;L23Yan40123.603TL61Dongnong42125.383T ;L24Jifeng260537305MTL62Daobei4315.463T ;L25Ji861135.405MTL63Ken9250973.217S

    ;L26Dongnong311418.953TL64Dongnong42391.629HS ;L27Dongnong921934.205MTL65Zupei64100.009HS ;L28Dongnong922068.637SL66Hanjiu95.899HS ;L29Qi~ng157.647SL67Xixuan166.007S

    ;L30Zhongmu3537.305MTL68Hejiang2394.629HS ;L31Jinfu2038.505MTL69Xinxue14.653T

    ;L32Dongnong71599.809HSL70Changlixiang48505MT ;L334227xFushiguang66.817SL71Shashani6.851HT

    273.877SL72Mu16147.305MT ;L34Hejiao91061

    ;L35Tongxi1207.681HTL73Song981084.

    ;587S

    ;L36Shishoubaimao33.605MTL74Pirmuo1985.377S ;L37Hejiang1931.105MTL75Xingzhimeng97.609HS ;L38Fushiguang95.409HS

    ;http:Hpublish.neau.edu.cn

    ;

    ;JournalofNortheastAgriculturalUniversityVo1.15No.12008 ;Discussion

    ;IntermsofJaponicaricevarietiesincoldregion,the ;identificationtestofcoldtoleranceatbudstageshow

    ;edthattherewasextremelyremarkabledifference ;ofsurvivingcelllivingability(theabilityoftheyoung ;budsinducedgreenseedling)amongricegermplasm ;resource.Theresultscarlprovidebasisforselectingthe ;varietieswhosegerminatingabilitywerestrongand ;theinducedgreenseedlingnumberwasthemoreat ;lowtemperaturefromricegermplasmresourceincold ;region.TheexperimentCallprovidethebasisforbreed- ;ingcoldtolerantvarietiesaswel1.

    ;Ricecoldtoleranceisacomplexquantitycharacter, ;differentchillinginjurysymptomswillemergeatdill- ;erentgrowthstage,thetolerantsymptomsofdifferent

    ;organsatlowtemperaturearedifferent,theeffect ;extentofchillinginjuryonthewholeplantatdifferent ;stageisdifferent[.Theidentificationtestofcoldtole.

    ;ranceatbudstageshowedthattherewereremarkable ;differenceoncoldtoleranceatbudstagebetweenthe ;maincultivatedvarieties(1ines)ofdifferenttimesand ;thericegermplasmresourcewhichcamefromdifferent ;ecologicalgeographicalregion.Theexperimentonly ;identifiedthecoldtoleranceatbudstageitshouldbe ;E-mail:xueba0engJjsh@neau.edu.cn

    ;furtherdiscussedthatwhethertherewereacorrelation ;betweencoldtoleranceatbudstageandthatatother ;growthstages,andacorrelationbetweenthecoldtole

    ;ranceatbudstageandotheragronomiccharacters.The ;experimentonlyidentifiedsomevarietiescoldtole

    ;ranceatbudstage,thewholegrowthstageidentifi

    ;cationshouldbedonetoevaluateavarietyfully.

    ;References

    ;1LiTG.Themethodsofselectingcoldtolerantvarietyofdifferent

    ;growthstageindooratlowtemperature[j].ForeignAgricultural ;Technology,1981(4):1821.

    ;2LiTG,ShenB.Analysisofrecoveryprocessofriceyoungseedl

    ;ingschillinginjuryatlowtemperature-roomtemperature[JJ_ ;ChineseAgriculturalScienceBulletin,1994,l0(4):13-16. ;3DaiLY,YeCR,XiongJH,eta1.Identificationandevaluation ;methodsofricecoldtolerance[JJ.ChineseJournalofRiceScience, ;1999,12(1):62.

    ;4HanLZ.ZhangSY.Identificationandevaluationmethodsofrice ;coldtolerance[JJ.JournalofPlantGeneticResource,2004,5(1): ;75.80

    ;5JinRZ.Studyonricecoldtoleranceidentificationandheredityin ;Japanese[RiceAbstract,1990,9(3):1-5.

    ;6XiongZMStudyonheredityofcoldtoleranceatseedlingstage ;ofearlyseasonIndiaricevarieties[JJ.ChineseJournalofRice ;Science,1990,4(2):7578.

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