Identification
June2008JournalofNortheastAgriculturalUniversityVo1.15No.21-4 ;IdentificationofColdToleranceofRiceGermplasmResourceat ;GerminatingStageinColdRegion
;LIUHualong,SUNShiche,WANGJingguo,andZOUDetang
;CollegeofAgriclture,NortheastAgriculturalUniversity,Harbin150030,China ;HeilongjiangAcademyofAgriculturalSciences,Harbin150086,China ;Abstract:Total75ricevarieties(1ines)inHeilongjiangProvince(orcoldregion)asgermpla
smresourceswereidentifiedforcold
;toleranceatgerminatingstagebycontrollingtemperatureinartificialincubator.Theresults
showedthattheshootingseedrateatthe
;germinatingstagecouldbeusedastheevaluationindexofcoldtolerance.Thecoldtolerance
wasrecordedon1-9scaleandcouldbe
;identifiedbvthecriteriaoffiveindexessuchashighlytolerant(HT),tolerant(T),moderatel
ytolerant(MT),susceptible(S),highly
;susceptible(HS).
;Keywords:rice,coldtolerance,germplasmresource,identification ;CLCnumber:$511:$426Documentcode:AArticleID:1006—8104(2008)一
02—0001—04
;Introduction
;RiceisoneofthemaincerealcropsinHeilongjiang
;Province.Thericegrowthperiodgetsshorterinthe
;temperatezoneofthisprovince.Chillinginjuryisone
;ofthemostnaturalfaminetoinducetheyielddeclinein
;riceproduction.Oneofthemosteffectivewaysofre—
;sistinglowtemperaturestressisricebreedingoflow
;temperaturetolerance.Itisreportedthatacloserela—
;tionofcoldtoleranceatgerminatingstage,booting
;stageandfloweringstageisobserved_lJ,therefore,the ;coldtoleranceatgerminatingstageiswidelyusedin
;theidentificationofficegermplasmresource.
;Inthispaper,75varieties(1ines)wereidentifiedfor
;coldtoleranceatgerminatingstagebycontrollingtem—
;peratureinartificialincubator.Coldtolerancechar—
;actersofthesematerialswerediscussedasatheoretica1 ;basisforthericechilling--tolerantbreedinginHeilong-- ;jiangProvince.
;MaterialsandMethods
;Materials
;Total75varieties(1ines)werecollectedbyRiceBreed—
;ingLabofNortheastAgriculturalUniversityasexperi- ;mentalmaterialswhichdistributedinmostriceregion ;andrepresentedbasicallythemaincategoriesofrice ;varietiesinHeilongjiangProvinceatpresent. ;Methods
;Tbtal100fullseedsfromeachmaterialwereputinto ;aculturedishwithfilterpaper.Thenthedisheswere
50?tomakethe ;keptinincubatorfor48hat45—
;seedsdryfullyandtheseedsdormancywasbroken. ;Afterseedsoaking,seedsbegantogerminatein ;incubatorat30.C.Whenthebudsreached5113111long, ;thetemperaturewasregulatedinto5~Cfor10days, ;thenthedisheswereputintoaroomofabove20?to
;makebudsgrownormallyfor7—10daysAfterall
;Receivedl7December2007
;LIUHualong(1978
一),male,Master,asistantlecturer,engagedintheresearchofricegeneticsbreeding.
mail:zondt@163.com ;Towhomcorrespondenceshouldbeaddressed.E—
;http://publish.neau.edu.c[i
;
;?2?JournalofNortheastAgriculturalUniversityVl-15No.22008
;thesesteps,thealiveseedlingswerecountedandthe ;survivalseedlingratewasformulated.Acomplete—
;lyrandomizedexperimentwascompletedwiththree ;repeats.
;Thesurvivalseedlingratesof75materialswere ;evaluatedasaindexofcoldtoleranceatgerminating ;stage.
;Thesurvivalrate(%)=(thesurvivalseedlings/the ;totalbuddingseeds)×100%
;ResultsandAnalysis
;Establishmentofscalesforcoldtoleranceat ;germinatingstage
;Thecoldtoleranceatbudstagewasdefinedasthe ;abilityforyoungbudstoremainaliveincoldwater, ;andappearedasanabilityofinductionofgreenseedl—
;ingfromyoungbud[3_4】.
;Therefore,thecoldtoleranceatbudstagewaswide—
;lyappliedincoldtoleranceidentificationofricegerm—
;plasmresource.
;Theevaluationofcoldtoleranceatbudstagewas ;madebywayofsurvivalseedlingrateandrecordedin ;5criteriasof1-9scalesuchashighlytolerant(HT), ;tolerant(T),moderatelytolerant(MT),susceptible(S),
;highlysusceptible(HS).Theclassificationcriteriaare ;showninTable1.
;Table1Classificationstandardofcoldtoleranceatbudstage ;Differenceofcoldtoleranceatgerminating ;stageamongvarieties
;Thecoldtoleranceof75materialsatbudstagewas
mail:xuebaoenglish@neau.edu.crl ;E—
;analyzedandshowninTable2,andextremelysigni—
;ficantdifferencesamongexperimentalmaterialsof ;coldtoleranceatbudstagewerefound.
;ThedeadseedlingrateofBinxuwasthelowest ;(6.45%)andthatofJinfu13,Zupei64wasthehigh—
;est(100%).
;Therangeof93.55%fromthelowesttothehighest ;showedtheextremelysignificantdifferencesincold ;toleranceatbudstageamongvarieties.
;Thedeadseedlingratesin10varietieswerebetween ;10%一30%andthatin24vailetieswerebetween30%一
;50%andin17varietiesbetween50%一90%andin14
;varietiesabove90%.
;Basedontheclassificationstandardofcoldtolerance ;atbudstage,thehighlytolerantvarieties(HT)with ;90%greenseedlingrateinducedfromyoungbudsand ;withdeepgreenleaveswereBinxu,Xueguang,Tongxi ;120,T0ngyu250,Shangyu397,Dongnong416,Song ;97—98,Song938,Shashani,Dongnong9601;thetole—
;rancevarieties(T)wereJifeng10,Xiaobaidao,Yan ;401,Teyou14,Yan105,Dongnong421,Daobei43, ;Xinxue,Xingjiao503,Dongnong3114;themoderately ;tolerantvarieties(MT)wereSong237,Fengqi1,4227x ;Fushiguang,Pinnuo19,Song98—10,Xixuan1,Ken92—
;509,Hejiao91061—2;thehighlysusceptiblevarieties ;(}IS)wereSui112,Longxuan948,99F一13,Fengyou303,
;Dongnong423,Jinfu19,Jinongda3,Jinfu13,Dongnong ;715,Fushiguang,Xingzhimeng,Hejiang23,Hanjiu, ;Zupei64;thesusceptiblevarieties(S)wereTeyou16, ;Fengyou503,Teyou14,Jinfu1,Jinfudao,Dongnong ;911,Nonglin20,Jinfu15,Dongnong418,Fengyou307, ;Teyou21,Dongnong98—25,Xuenuo,Caidao,Tongyu
;139,Jifeng2605,Ji86—11,Dongnong92—19,Zhongmu
;35,Jinfu20,Changlixiang,Hejiang19,Mu16—1,Bai—
;shoushimao.
;Experimentalresultsshowedthatthechillinginjury ;reactionofdifferentricevarietiesunderlowtempera—
;turewasveryobvious,andhadgoodreproducibility. ;Thereforeitispossibletoselectthcstableandreliable
;coldtolerancevarietiesbytheidentificationofcold ;toleranceofdifferentricevarietiesatgerminating ;stage.
;
;LIUHualongela1.IdentificationofColdToleranceofRiceGermplasmResourceatGermi
natingStageinColdRegion
;Table2Differencesofcoldtoleranceatbudstageamongvarieties
;N..VarietyDea
;mt
;d
;e
;se
;r
;e
;%
;d
;1
;li”gsca1eMarkN..VarietyDea
;rat
;d
;e
;se
;r
;e
;%
;d
;1
;lings
;ca1eMark
;LO1Jifeng1016.583TL39Song23760.647S ;L02Teyou1640.005MTL40Song26966.407S ;L03Fengyou50334.805MTL41Song9388.561HT ;L04Sui11294-359HSL42Teyou1425.503T ;L05Longxuan94890.859HSL43Fengyou30734.205MT ;L0699F一1396.029HSL44Fengyou30179.457S ;L07Teyou1444.805MTL45Fuyuan470.027S ;L08Fengyou30399819MSL46Teyou2141.605MT ;L09Jinfu148.505MTL47Dongnong98—2532.305MT
;LIOTongyu2509.521HTL48Teyou1368.277S ;L11Jinfudao49105MTL49Song97—987.861HT
;L12Jinfu1991-359HSL50Dongnong96018.671HT ;L13Jinongda399509HSL51Xueguang6.581HT
;L14Dongnong911943.505MTL52Jingyin5963.657S ;L15Nonglin2034.805MTL53Shangyu3977.951HT ;L16Zupei282.047SL54Yan10518.623T
;L17Jinfu13100.009HSL55Dongnong4168.641HT ;L18Jinfu1535.605MTL56Xuenuo34.705MT
;L19Dongnong41834.805MTL57Binxu6.451HT ;L20Xingjiao50326.413TL58Caidao45.405MT ;L21Fengyuan60356637SL59Tongyu13948005MT
;L22Xiaobaidao16.703TL60Kenxiaodao78.097S;L23Yan40123.603TL61Dongnong42125.383T ;L24Jifeng260537305MTL62Daobei4315.463T ;L25Ji86—1135.405MTL63Ken92—50973.217S
;L26Dongnong311418.953TL64Dongnong42391.629HS ;L27Dongnong92—1934.205MTL65Zupei64100.009HS ;L28Dongnong922068.637SL66Hanjiu95.899HS ;L29Qi~ng157.647SL67Xixuan166.007S
;L30Zhongmu3537.305MTL68Hejiang2394.629HS ;L31Jinfu2038.505MTL69Xinxue14.653T
;L32Dongnong71599.809HSL70Changlixiang48505MT ;L334227xFushiguang66.817SL71Shashani6.851HT
273.877SL72Mu16—147.305MT ;L34Hejiao91061—
;L35Tongxi1207.681HTL73Song98—1084.
;587S
;L36Shishoubaimao33.605MTL74Pirmuo1985.377S ;L37Hejiang1931.105MTL75Xingzhimeng97.609HS ;L38Fushiguang95.409HS
;http:Hpublish.neau.edu.cn
;
;JournalofNortheastAgriculturalUniversityVo1.15No.12008 ;Discussion
;IntermsofJaponicaricevarietiesincoldregion,the ;identificationtestofcoldtoleranceatbudstageshow—
;edthattherewasextremelyremarkabledifference ;ofsurvivingcelllivingability(theabilityoftheyoung ;budsinducedgreenseedling)amongricegermplasm ;resource.Theresultscarlprovidebasisforselectingthe ;varietieswhosegerminatingabilitywerestrongand ;theinducedgreenseedlingnumberwasthemoreat ;lowtemperaturefromricegermplasmresourceincold ;region.TheexperimentCallprovidethebasisforbreed- ;ingcold—tolerantvarietiesaswel1.
;Ricecoldtoleranceisacomplexquantitycharacter, ;differentchillinginjurysymptomswillemergeatdill- ;erentgrowthstage,thetolerantsymptomsofdifferent
;organsatlowtemperaturearedifferent,theeffect ;extentofchillinginjuryonthewholeplantatdifferent ;stageisdifferent[一.Theidentificationtestofcoldtole.
;ranceatbudstageshowedthattherewereremarkable ;differenceoncoldtoleranceatbudstagebetweenthe ;maincultivatedvarieties(1ines)ofdifferenttimesand ;thericegermplasmresourcewhichcamefromdifferent ;ecologicalgeographicalregion.Theexperimentonly ;identifiedthecoldtoleranceatbudstageitshouldbe ;E-mail:xueba0engJjsh@neau.edu.cn
;furtherdiscussedthatwhethertherewereacorrelation ;betweencoldtoleranceatbudstageandthatatother ;growthstages,andacorrelationbetweenthecoldtole—
;ranceatbudstageandotheragronomiccharacters.The ;experimentonlyidentifiedsomevarietiescoldtole—
;ranceatbudstage,thewholegrowthstageidentifi—
;cationshouldbedonetoevaluateavarietyfully.
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