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(DOC) Expression_1

By Beatrice Palmer,2014-07-22 02:58
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Expression_1

    Expression

Inductionofumbilicalcordbl00dderivedbeta2m-c-Met+cellsinto

    ;hepatocyte..likecellsbycoculturewithCFSC/HGFcells

    ;WangNanX,LiZhangRYueYanF,PeiX.

    ;DepartmentofStemCellBiology,BeijingInstituteofTransfusionMedicine,Beijing,China

    ;Severalstudieshave1ndicatedthatadultstemcellsderivedfrombonemarrow(BM)andcordblood(CB)can

    ;differentiateintohepatocytelikecells.ThisabilityisimportantforthetreatlnentofhepaticdiseaseswithBMorCB

    ;asapotentialapproach.However,methodsarestillbeingdevelopedfortheefficientinductionofstemcelldifferen

    ;tiationandexpansiontogetenoughcellstobeusefil1.Inthepresentstudy,weenrichedasubsetofumbilicalcord

    ;bloodbeta(2)m()cMet(+)cells(UCBCCs)andinvestigatedthecombin

    ationeffectoflivernonparenchymalcells

    ;(cirrhoticfatstoringcells[CFSCs])andllepatocytegrowthfactor(HGF)ontheinductionofUCBCCsintohepato

    ;cytelikecells.UCBCCswerecoculturedwithCFSC/HGFfeederlayerseitherdirectlyorseparatelyusinginsert

;wells.FlowcytometricanalysisshowedthatmostUCBCCswereCD34(

    /_)CD90(+/)cD49f(+)CD29(+)Alb(+)

    ;AFP(+).Aftercoculturedwithtransgenicfeederlayersfor7days,UCBCCsdisplayedsomemorphologiccharacter

    transcriptionpolymerasechainreaction(RT;isticsofhepatocytes.Reverse

    PCR)andinununofluorescencecell

    ;stainingprovedthattheinducedUCBCCsexpressedseveralhepatocytespecificgenesincludingAFP,Alb,CYP1B1

    ;andcytokeratinsCK18andCK19.Furthermore,theinducedcellsdisplayedliverspecificfunctionsofindocyanine

    ;green(ICG)uptake,alranonitlmmetabolismandalbuminsecretion.Hence,ourdatahavedemonstratedthatUCBCCs

    ;mightrepresentanovelsubpopulationofCB’derivedstem/progenitorcellscapableofsuccessfuldifferentiationinto

    ;hepatocytelikecellswhenincubatedwithCFSC/HGFcells.Inconclusion,notonlyHGFbutalsoCFSCsand/orthe

    ;secretedextracellularmatrix(ECM)havebeenshowntobeabletoserveasessentialmicroenvironmentforhepato

    ;cytedifferentiation.

    ;LiverTransp1.2005,11(6):635._643

    ;Expressionprofilesofmousedendriticcellsarcomaaresimilartothoseof ;hematopoieticstemcellsorprogenitorsbyclusteringandprincipal

;componentanalyses

    ;ZhouS,LiuY,WengJ,KongL,SunX,GuB,LuZ.

    ShiungWuLaboratory,DepartmentofBiologicalScienceandMedi;Chien

    calEngineering,SoutheastUniversity,Nanjing210096,China. ;WeisolatedandscreenedtwotumorcellclonesDD1andDG6withdifferentcapacityofmetastasisfromthe

    ;sameparentcellline,alnousedendriticcell(DC)sarcoma,usinglimiteddilutionmethod.Thegenomewideexpres

    ;sionsofDD1andDG6cellsweredetectedbyAffymetrixMOE

    430Amicroarray.Theexpressionprofiles

    ;relatedwithmouseDCdevelopmentweredownloadedfromGEOatNCBIandArrayExpressatEBIdatabase.In

    ;ordertocomparetheexpressionofDCsarcomaandDCdevelopmentalarrayswhichwasperformedbyMG

    ;

    ;13

    ;

    ;U74av2,wehadscreenedthebestmatchedprobesetsbetweenMOE

    430AandMGU74av2accordingtotheprobe

    ;identitiesfromAffymetrixteclmicalannotation.Afterthenormalizationof11housekeepinggenesacrossthe34

    ;arrays(2DCsarcomaand32DCdevelopmentalarrays),alltheseexpressionp

rofileswereanalyzedbythemethods

    ;ofhierarchicalclustering,principalcomponentanalysis,nearestneighbor

    hood,andself-organizingmaps.There.

    ;sultsindicatethatexpressionprofilesofDCsarcomaareclosertothoseoftheDCprogenitorsandhematopoietic

    ;stemcellsfrombonemarrowcomparedwiththesortedDCsfromspleen.Theresultssupportthehypothesisthat

    ;cancers(tumorsorsarcomas)arisefromstemcells.ItiSsuggestedthattheDCsarcomasaremoresimilartothe

    ;DCprogenitorsandhematopoieticstemcellsthantherelativematureDCsingeneexpressionsonthelarge-scale.

    ;BiochemBiophysResCommun.2005,331(1):194_202

    ;Cytokinestransducedbonemarrowstromalcelllinespromote ;immunohematopoieticreconstitutioninmiceafterallogeneicbonemarrow ;tranSplantati0n

    ;LiA,JiangJ,ZhangQ,HaoJ,XieS.

    ;DepartmentofImmunology,PekingUniversityHealthScienceCenter,Beijing,China

    ;ImpairedimmunereconstitutionfollowingallogeneicT-celldepletedbonelrmrrowtransplantationralloTCD

    ;BMT)iSamajorobstacletoitsclinicalapplication.Stroma1cell1ineQXMSC1,establishedfrombonemarrowcells

;ofBALB/c(H2d),wastransfectedwithmurineIL3and/orIL

    2gene,andinjectedintolethallyirradiatedC57BL/ ;6(H2b)mice.Weevaluateditseffectsoninmaunologicandlaematopoieticrec

    onstitutionafterallo-TCDBMT.The

    ;resultsshowedthatOXMSC1IL3IL

    2couldsignificantlyincreasethelmmbersofhematopoieticprimitive

    ;progenitors(CFUS),committedprogenitors(CFUGM,andBFUE),

    andlymphocytes(CD8+cells,CD4+cells,and ;Bcells).Similarly,immunefunctionsofrecipientmiceweresignificantlyerd

    aancedintheQXMSC1IL3+IL2

    ;group.Inaddition.QXMSC1IL3orQXMSC1IL

    2alsoexertedapparenteffectsonacceleratingimmune ;reconstitution.buttheseeffectswerefarlessthanthatofQXMSC1IL

    3+IL2.OBrresultsdemonstratedthat

    ;stromalcellmediatedIL3andIL

    2genetherapymaybeapotentapproachinpromotingimmunologicandhe

    ;matopoieticreconstitutionafteralloTCDBMT.

    ;1mmunolLett.2005,98(2):216_224

    ;

    ;

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