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(DOC) Engrafted

By Mildred Rivera,2014-07-22 02:56
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Engrafted

    Engrafted

Engraftedbonemarrowderivedflk

    (1+)mesenchymalstemcellsregenerate

    ;skintissue

    ;DengW,HanQ,LiaoL,LiC,GeW,ZhaoZ,YouS,DengH,MuradF,ZhaoRC. ;InstituteofBasicMedicalSciences,CenterforTissueEngineering,ChineseAcademyofMedicalScienceandPekingUnionMedica

    ;College,Beijing,China.

    ;Stemcellplasticityhascreatedgreatinterestbecauseofitspotentialtherapeuti

    capplicationilldegenerativeor

    ;inheriteddiseases.Transplantationofbonemarrowderivedstemcellswass

    howntogiverisctocellsofmuscle.

    ;1iver,nerve,endothelium,epithelium,andsoon.Buttherearestilldisputesab

    outstemcellplasticity,especiallycon

    ;cerningthecontributionofbonemarrowderivedcellstoskincells.Inthisstudy,CMDiIfluorescencelabeled

    ;Flk

    (1+)bonemarrowmesenchymalstemcells(bMSCs)ofBALB/cmice(H

    2Kd,white)weretransplantedinto

    ;lethallyirradiatedC57BL/6l-nice(H

    2Kb,black).Byfluorescencetracing,wefoundthatdonorcellscouldmigrate ;andtakeresidencyattheskin,whichwasconfirmedbyYchromosome-specificPCRandSouthernblot.The

    ;recipientmicegrewwhitehairsabout40dayslaterandwhitehairscouldspreadoverthebody.In~nunochemistry

    ;stainingandRT——PCRdemonstratedthatskintissuewithinthewhitehairregionswaslargelycomposedofdonor——

    ;derivedH

    2Kdcells,includingstemcellsandcorrmaittedcells.Furthermore.mostskincellsculturedfromwhitellair

    ;skinoriginatedfromthedonor.Thus,ourfindingsprovidedirectevidencethatbonemarrowderivedcellscallgive

    ;risetofunctionalskincellsandregenerateskintissue.Thesemayhaveimportantscientificimplicationsinstemcell

    ;biologyandtransplantationtherapyforskintissueinjury.

    ;TissueEng.2005,11(12):110119

    ;Roleofsonichedgehoginmaintainingapoolofproliferatingstemcellsin ;

    ;thehumanfetalepidermis

    ;ZhouJX,JiaLW,LiuWM,MiaoCL,LiuS,CaoYJ,DuanEK.

    ;StateKeyLaboratoryofReproductiveBiology,InstituteofZoology,ChineseAcademyot’Sciences;GraduateSchoolot.tile

;ChineseAcademyofSciences,Beijing,China,

    ;BACKGROUND:Then~alrunalianepidermisismaintainedbytheongoingproliferationofasubpopulationof

    ;keratinocytesknownasepidermalstemcells.Sonichedgehog(Shh)canregulatemorphogenesisofhairfollicles

    ;andseveraltypesofskincancer,buttheeffectofShhonproliferationofhumanputativeepidermalstemcells

    ;(HPESCs)ispoorlyunderstood.METHODSANDRESULTS:WefirstfoundthatShh,itsreceptorsPatchedl(Ptc1)

    ;aswellasSmoothened(Smo)anditsdownstreamtranscriptionfactorGli

    1wereexpressedinthebasallayerof

    ;;,

    J,fetalepidermisandfreshlysortedHPESCs.Next.treatmentofHPESCswithmediaconditionedbyShhN

    ;expressingcellspromotedcellproliferation,whereasinhibitionofShhbycyclopamine,aspecificinhibitorofShh

    ;signalling,hadanoppositeeffect.Interestingly,themitogeniceffectofepidermalgrowthfactor(EGF)onItPESCs

    ;wasefficientlyabolishedbycyclopamine.Finally,bonemorphogeneticprotein4(BMP4),apotentialdownstream

    ;effectorofShhsignalling,increasedHPESCproliferationinaconcentrationdependentmanner.CONCLUSIONS:

;

    ;71 ;

    ;

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