Epidermal
ShhisanimportantregulatorofHPESCproliferationinthebasallayerofhumanfetalepidermisandmodulatesthe
;cellresponsivenesstoEGF,whichwillassisttounravelthemechanismsthatregulatestemcellproliferationand
;neoplasiainthelmmanepidermis.
;HumReprod.2006Mar29;[Epubaheadofprint]
;Epidermalstemcellsarethesourceofsweatglandsinhumanfetalskin: ;evidenceofsynergeticdevelopmentofstemcells,sweatglands,growth ;factors,andmatrixmetallOprOteinases
;FuX,LiJ,SunX,SunT,ShengZ.
;WoundHealingandCellBiologyLaboratory,BurnsInstitute,304ClinicalDepartment,TheGeneralHospitalofPLA,Trauma
;CenterofPostgr=duateMedicalCollege,Beijing,China
;Thedevelopmentofsweatglandsisaconplexbiologicalprocess,andtheextentofcellulartraffickingbetween
;epidermalstemcellsandthedevelopmentfsweatglandsisuncertain.Therefore,westudiedthesynergeticdevel—
;opmenteffectsofstemcells,sweatglands,growthfactors,andmatrixmetalloproteinases(MMPs)inhumanskin.
;Humanfetalskinwasobtainedfromspontaneouslyabortedfetusesat11—31
weeksofgestation.Para俺nsections
;werecutandstainedwithhematoxylinandeosinorilnmunostainedwithantibodiesagainstbeta(1)integrin,keratin
;(K)一19andK7,MMP一2mad一
7,andepidermalgrowthfactor.Insituhybridizationwasttsedalongwithsemiquantitative
;analysisofthepositiveexpressionoftheseproteinstoanalyzeformRNAexpressionofMMP一2and一7.Histological
;studiesrevealedthefetalepidermisbegantoformaprimaryepidermalridgeatgestationalage13’.14weeksand
;theseprimordialbasalcellsbecametightlypackedtotaketheformofmultiplehillocksbetween14and16weeks.
;Furthermore,thesecellsgaverisetochord—likecolumnarbudsintheembryo
nicepidermis,andthesebudsgradually
;migrateddownwardintothedermistoformjuvenilesweatglandsat18—20w
eeks.Maturesweatglandswerefound
;inthefetalepidermisattheendof24weeks.beta(1)integrinandK19immunoreactivitieswerefirstdetectedin
;thosecellsthatgatheredtogethertoformprimaryepidennalridges,includingsweatglandcords,buds,andimmature
;sweatglandcells.ThepositiveimmunostainingforK7appearedinearlysweat
;
;