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(DOC) Epidermal

By Jonathan Wood,2014-07-22 02:56
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Epidermal

    Epidermal

    ShhisanimportantregulatorofHPESCproliferationinthebasallayerofhumanfetalepidermisandmodulatesthe

    ;cellresponsivenesstoEGF,whichwillassisttounravelthemechanismsthatregulatestemcellproliferationand

    ;neoplasiainthelmmanepidermis.

    ;HumReprod.2006Mar29;[Epubaheadofprint]

    ;Epidermalstemcellsarethesourceofsweatglandsinhumanfetalskin: ;evidenceofsynergeticdevelopmentofstemcells,sweatglands,growth ;factors,andmatrixmetallOprOteinases

    ;FuX,LiJ,SunX,SunT,ShengZ.

    ;WoundHealingandCellBiologyLaboratory,BurnsInstitute,304ClinicalDepartment,TheGeneralHospitalofPLA,Trauma

    ;CenterofPostgr=duateMedicalCollege,Beijing,China

    ;Thedevelopmentofsweatglandsisaconplexbiologicalprocess,andtheextentofcellulartraffickingbetween

    ;epidermalstemcellsandthedevelopmentfsweatglandsisuncertain.Therefore,westudiedthesynergeticdevel

    ;opmenteffectsofstemcells,sweatglands,growthfactors,andmatrixmetalloproteinases(MMPs)inhumanskin.

;Humanfetalskinwasobtainedfromspontaneouslyabortedfetusesat1131

    weeksofgestation.Paransections

    ;werecutandstainedwithhematoxylinandeosinorilnmunostainedwithantibodiesagainstbeta(1)integrin,keratin

    ;(K)19andK7,MMP2mad

    7,andepidermalgrowthfactor.Insituhybridizationwasttsedalongwithsemiquantitative

    ;analysisofthepositiveexpressionoftheseproteinstoanalyzeformRNAexpressionofMMP2and7.Histological

    ;studiesrevealedthefetalepidermisbegantoformaprimaryepidermalridgeatgestationalage13’.14weeksand

    ;theseprimordialbasalcellsbecametightlypackedtotaketheformofmultiplehillocksbetween14and16weeks.

    ;Furthermore,thesecellsgaverisetochordlikecolumnarbudsintheembryo

    nicepidermis,andthesebudsgradually

    ;migrateddownwardintothedermistoformjuvenilesweatglandsat1820w

    eeks.Maturesweatglandswerefound

    ;inthefetalepidermisattheendof24weeks.beta(1)integrinandK19immunoreactivitieswerefirstdetectedin

    ;thosecellsthatgatheredtogethertoformprimaryepidennalridges,includingsweatglandcords,buds,andimmature

    ;sweatglandcells.ThepositiveimmunostainingforK7appearedinearlysweat

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