The studies on the laxative effect of honey
HU Dan LIN Lishan ZHUANG Hong ZHOU Li CHENG Liang
Class 1,Grade 00,Department of Clinic Medicine Sun Yat-sen University,Guangzhou 510089 R.P.China
Department of Physiology Sun Yat-sen University,Guangzhou 510089,P.R.China
，Abstract！ Objective To investigate the effect of honey on laxatation of mice. Methods
Measure the distance the honey(mixed with carbon) covers and record the time of defecation after giving the honey of different concentration by the gut. Results 100% and 50% honey has obvious positive effect on the
defecation of mice and greatly shorten the time of laxatation . Conclusions Honey can effectively promote
intestinal movement and shorten the laxatative time, so it can be a propriate medicine for astriction.
，Key words！ Honey Effect of defecation
Nowadays, with the advancement of life level, people are more and more intent to gain health from nutrition.There are various kinds of good for health care on sale. And some are necessity of municipal life. Such as honey.
It is shown from investigate that the live rhythm of modern municipal life is too intensive. Many people are burden with high mental pressure, and in addition, their food is poor in nutrition, and most are fast-food, such as hamburger and sandwich. These unadvisable life customs have bad effect on our health. Gastrointestinal disorder, like astriction, is one of these effects. It is said that honey can promote defecation, but what is the mechanism, how effective honey is, this is the objection of our experiment.
Bee honey is the honey brewed by bee, it is stiff fluid, with color of white to light yellow. Honey is widely uses in medical field, it is recorded in “Ben Cao” that honey has function of moistening, and can smooth intestine.
It can be used for the therapy of astriction.
2 Materials and Methods
Experimental object: 48 white rat(18-22g)
Experimental equipment and medicine: Hangzhou honey, eosin, dust of char, distilled water, picric acid, 6
Experiment 1. Effect of honey on the intestinal impellent movement of mice
Take 24 mice, half male and others female, 18 to 22g, divide them into 4 groups, 6 each group, supply them with water, but fasting. 16 hours later, group 1-3 are clystered with honey mixed with 5%dust of char. The concentration of honey is 100%, 50% and 25%, and the control group of mice are clystered with distilled water mixed with 5% char, 20 mins later, execute the mice, measure the length of the boosting length of char and calculate percentage of boosting char in intestine.
Experiment 2. Effect of honey on defecation of mice
Take 24 mice, half male and others female, 18 to 22g, divide them into 4 groups, 6 each group, group 1-3 are
thclysterd with honey of 100%, 50% and 25% concentration. The 4 are control group(feed with distilled
water).Each groups of honey of different concentration and distilled water should dissolve with 1% eosin. After
that, feed the mice separately, record the time when mouse defecate the first red stool. The interval time from clysis to defecation is the defecation time of the mouse.
2.3 Experimental index The boosting degree of dust of char in the intestine of mice and the defecation time after clysis.
Table 1.1 Effect of honey on the intestinal impellent movement of mice(comparison between
experiment group and control group)
Group Number of animal Boosting degree Value P
6 0.722?0.021 P<0.01 Honey (100%)
6 0.59?0.026 P<0.01 Honey (50%)
6 0.544?0.005 P>0.05 Honey (25%)
6 0.459?0.020 Control group
Table 1.2 Effect of honey on the intestinal impellent movement of mice(multiple comparison
between three experiment groups)
Group Number of animal Difference of boosting Value P
Group 100% and 50； 6 0.132 P<0.01
Group 100； and 25； 6 0.178 P<0.01
Group 50； and 25； 6 0.046 P<0.01
From table 1, we can conclude that when comparing the control group with honey groups of 100% and 50% concentration, the difference is obvious (P<0.01). it show that honey has obvious promotive effect on the intestinal impellent movement of mice, 25% honey has no obvious effect. In addition, boosting degree of honey group of 100% and 50% concentration is of statistically significant difference (P<0.01). it indicates that the group 100% concentration tends to have better effect on the intestinal impellent movement.
Table 2.1 Effect of honey on laxatation of mice (comparison between experimental group and control group)
Laxatation time？min？ Group Number of animal Value P
Honey？100%？ 6 43.6?25.3 P<0.01
Honey？50%？ 6 49.8?13.3 P<0.01
Honey？25%？ 6 75.7?9.16 P<0.01
Control group 6 129.7?14.3
Table 2.2 Effect of honey on laxatation of mice (multiple comparison between experimental groups)
Group Number of animal Difference of mean time(min) Value P
Honey 100； and 50； 6 6.2 P<0.01
Honey 100； and 25； 6 32.1 P<0.01
Honey 50； and 25； 6 25.9 P<0.01
From table 2, we can conclude that the laxatation time of 100% and 50% honey basically range from 40 to 90 min, while the control group is 129.7 min, the difference are very prominent (P<0.01). It shows that the honey has good laxative effect. in addition, the laxative time of every two groups of the experimental group is of statistically significant difference (P<0.01). It indicates that the honey of higher concentration tends to have better effect on shortening the laxative time.
Most of the honey’s component are fruct and glucose (70%). Others are balata, organic acid, enzyme, inorganic salt. In the experiment, we discover that:
1 In the experiment of intestinal boosting movement, the effect of honey with 50% and 100%
concentration is prominently boosting different from the control group, but 25% honey group has no
prominence from control group. It shows that the higher the honey’s concentration is, the more
prominent the effect is.
2 When measuring the laxative time, we discover that 25% honey can also have prominent effect. It may
result from the boosting effect of honey is better than that of char. So low concentration honey can also
shorten laxative time.
3 The experimental result show tat honey can obviously boost up the intestinal squirm, accelerate
laxatation. In other hand, all stool are shaped stool, there are no watery stool. So it suggests that the
effect of honey is tempered, so it is a decent medicine used for the therapy of astriction of pregnant
women and seniors.
4 Some acid components of honey such as acetic acid and hydrochloric acid can promote digestion. Other
components such as enzyme, colloid and waxiness can accelerate the digestion, and shorten the time
injecta stay in the intestine, it can also prevent astriction.
The experimental result shows that honey can effectively promote intestinal movement and shorten the laxative time. Honey has function of adjusting gastrointestinal tract. So honey can be used as a propriate medicine for therapy of astriction.
References(refer to Chinese version)