Sampling & transportation

By George Bryant,2014-08-28 20:06
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Sampling & transportation

Sampling & transportation

    Major topics

    ; Sampling

    ; Preparing and packaging

    ; Important rules for diagnostics and dangerous specimens

    ; Transporting

    ; Practical sessions on sampling (gray cells in the schedule)

Expected outcome

    At the end of the module, the participant should:

    ; Know and use sampling material and supplies

    ; Be able to perform different types of samples

    ; Sample appropriately in accordance to good biosafety rules

    ; Identify sample, record and store it for conservation before analysis or transport, applying

    knowledge in sample management and recording

    ; Pack a sample before transportation appropriately in accordance to transportation rules

Tentative schedule

    Day Morning Afternoon

    1 ; Sampling premises and conditions ; Sample identification, logbooks and

    recording ; Sample material and equipment

    ; Sample acceptation/rejection and ; Refreshing in biosafety

    quality control

    ; Sample conservation

    2 ; Containment levels ; Theory on sampling (sampling by

    itself) ; Transport media

    ; Theory on sampling (sample

    preparation before analysis or


    ; distribution of the SOPs in sampling 3 ; distribution of the SOPs for ; International rules for dangerous

    transportation goods transportation

    ; Training on sample packaging and ; National rules for dangerous goods

    transporting transportation

    ; Training on sample reception ; Examples of sample transportation


    4 ; Stool sample ; CSF sample

    ; Blood sample ; Respiratory sample

    5 ; Urine sample ; Vaginal/urethral sample

    ; Nose/throat sample ; Conclusion of the training

Teaching material

    ; Presentations

    o Sampling

    o Sample transportation

    o Sample rules

    ; SOPs for sampling

    o Sampling rooms

    o Sampling material and supplies

    o Criteria of sample acceptance/rejection

    o Blood sample

    o Stool sample

    o Urine sample

    o Vaginal sample

    o Urethral sample

    o STD samples

    o Sputum sample


    o Wound sample

    o Ear sample

    o Nose sample

    o Throat sample

    o CSF sample

    o Lymph node sample

    o Mycology samples

    o Medullar samples

; SOPs for sample transportation

    o General facts about transport media

    o General facts about sample conservation

    o International transportation rules (IATA)

    o Specimen shipment procedures

    o Specimen reception procedures

    o Cary Blair media

    o Alkaline peptone water

    o TI media

    o Other transport media

    o Particular cases: HIV, hepatitis, AFP, VHF…

    Material needed

    A set of sampling material: (for both demonstration and practical)

    ; Cary-Blair transport media and collection swabs ; TI bottle transport media and CSF collection kit ; Blood culture bottles with SPS and blood collection kit

    ; Slides and lancettes

    ; Filter paper and sterile normal saline ; Serum tubes, labels

    ; Transport packaging materials (650 & 602) ; Insulated box with reusable cool packs ; Standardized request forms


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