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By Billy Harper,2014-02-18 17:53
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Possible

    Possible

    Secondarystructuralformationofalphasynucleinamyloidsasrevealed

    byg-factorofsolidstatecirculardichroism.

    Li11XJ,ZhangF,XieYY'BaoWJ,HeJH,HuHY.

    KeyI,abm'atoryofProteomics,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,Chinese

    AcademyofSciences,Shanghai20003I,China.

    alphaSynuclcin(alpha

    Syn)hasbeenidcntificdasacon]po1]entofilatracellularfibrillardepositsinParkinson's disease,Tlmughthercalpathogencsisisstillullkllown,nlanyinvestigationshaverevealedthatconforlnational

    alterationandtibrilforlnationofalpha

    Synpl'oteinhaveallilnportantroleincausingthedisease.Inthiswork,we introducedthcgfactorspectraofsolid

    statecirculardichroismtoestilnatetimsecondarystructurecontentsof

    alphaS,ynfl'agmentsinamyloids.Fouriertransfonninfrared(FT

    IR)wasalsoappliedtoconfirn]thestructural

    fol'lnati()1],Thcrcsultssuggestthatthcccntralhydrophobicrcgioniscriticalforbcta

    sheetformationandthe

    conforlnalionalalterationisthefoundationofproteinabnormalaggregation.Theresearchprovidesapracticalap

    proachtoestimatcthcsecondarystructurecontentsofproteinamyloidsandfllrtherinsightintotherelevanceof

    structul'altranslbnnationandamyloidogcncsis.(c)2006WileyPeriodicals,Inc.Biopolylners,2006,

    Biopolymers.2006Jt1112;Epubaheadofprint](ilnpactFactor:2,545)

    PossibleinvolvementofCa2+signalinginrotenoneinducedapoptosisin

humanneuroblastomaSHSY5Ycells.

    WangXJ,XuJX.

    InstituteofBiophysics,ChineseAcademyofSciences,Beijing10010I,China Rotcn()nc,allinhibitoroflnitochondrialrespiratorychainCOlnplcxI,isausefultooltoclicitanimallnodelofParkinso1]s

    disease,Rotenone

    indtmcdlaeuronalapoptosismaycontributetotheetiologyofParkinson'sdiscasc.Itowcvcr,the

    nlechanislnofrotenonc

    inducedapoptosisisnotthllyunderstood,Inthcpresentstudy.weshowthatCa2+signal

    ingisesscntialforrotenoneinducedapoptosisinhulllanneuroblastolnaSI'I

    SY5Ycells.ByusingFluo-3/AMand

    Fura-2/AM,thefluorescentcalciulnindicator,rotenonewasfoundtocausearisei11intracelhllarfleeCa2+([Ca2]i).

    TheintracelhdarCa2+chelatorBAPTAattelmatcdrotenonc

    induccdapoptosis.Notably.Ca2+suppressionalso

    prevcl1tcdrotcnone

    induccdapoptoticrclatcdevcntsincludingrcactivcoxygenspeciesproductiO1],G2/Mcellcycle

    arrestandcaspascactivation,suggestingthatCa2+signalingisupstrealntotheseevents.Intheabsenceofextracel

    hdarCa2I,therotenoncinduccdCa2ielevationwasinhibited.Further,thcvoltage

    dependentCa2chanlacl

    blockernifedipinesuppressedmostofthcelevationoffCa2I]iinducedbyrotenonc.Thcscrestdtsdcnlollslratethat

    rotcnoneleadstoanelevationinCa2]ithrouglaCa2-I_influxbythcopcningofvoltage

    gatcdCa2channc1.This

    studyl【】

    frotenonclnayIMptoelucidatethcl1curodcgcl1crativelneclaaninlsillParkinson'sdiscasc.

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