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Inoculation with phosphate-solubilizing fungi diversifies the bacterial community in rhizospheres of maize and soybean

By Dan Campbell,2014-02-18 10:39
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Inoculation with phosphate-solubilizing fungi diversifies the bacterial community in rhizospheres of maize and soybean

    Inoculation with phosphate-solubilizing fungi diversifies the bacterial community

    in rhizospheres of maize and soybean Symbioticbacteriaasadeterminantofplantcommunitystructureand

    plantproductivityindunegrassland

    vanderHeijdenMGA,BakkerR,VerwaalJ,ScheublinTR,RuttenM,vanLogtestijnR,StaehelinC

    VrijeUnvAmsterdam,InstEcolSci,NLJ08JHVAmsterdam,Netherlands

    SunYatSenUniv,StateKeyLabBiocontrol,SchLifeSci,Guangzhou,PeoplesRChina vanderHeijdenMGA(reprintauthor),VrijeUnivAmsterdam,lnstEcolSci,Boelelaan1085,NL1081HVAmsterdam,Netherlands

    marce1.van.der.heijden@ecology.falw.vu.nl

    ?

    Abstract:Symbioticinteractionsarethoughttoplayakeyroleinecosystems.Empiricalevidencefortheimpactof

    symbioticbacteriaonplantcommunitiesis,however,extremelyscarcebecauseofexperimentaIconstraints.Here,

    inthreecomplementaryexperiments,weshowthatnitrogen

    fixingrhizobiabacteriaactasadeterminantofplant

    communitystructureanddiversity.Grasslandmicrocosmsinoculatedwithamixtureofrhizobiahadahigherabove.

    groundplantproductivity(+35%),containedmorenitrogen(+85%)andhadsignificanthighercommunityevenness

    (+34%)thancontrolmicrocosmswithoutrhizobia.Moreover,threeofthefourstudiedlegumespeciesrequired

    rhizobiatosuccessfullycoexistwithotherplantspecies.Incontrast,thegrowthandsurvivalof

threegrassandfive

    forbspecieswerenotaffectedbythepresenceorabsenceofrhizobia.Finally.ourresultsalsoshowedthatthe

    legumespecieslargelyreliedonsymbioticallyfixednitrogen.bothinthefieldandinthemicrocosms.Thisindicates

    thatresultsinthemicrocosrnsareindicativeforprocessesoccurringinthefield.Itisconcludedthatsymbiotic

    interactionsbetweenplantsandprokaryotescancontributetoplantproductivity,plantcommunitystructureand

    acquisitionoflimitingresourcesinlegumerichgrasslandcommunities.

    Keywords:ecosystemfunction;microbialdiversity:naturalabundanceOfN

    15;nitrogenfixation;nitrogentransfer;

    speciesrichness

    LEGUMrNOSARUMBVTRIFOLII;16SRIBOSOMALRNA;RHIZOBIUM

    LEGUMINOSAR1M:DIVERSITY;

    NITROGEN:LEGUMES;BIOD?ERSITY:ABUNDANCE;ECOLOGY:SYSTEMS

    FEMSMICROBIOLOGYECOLOGY2006,56(2):178187

    Inoculationwithphosphatesolubilizingfungidiversifiesthebacterial

    communityinrhizospheresofmaizeandsoybean

    WangGH,JinJ,XuMN,PanXw'TangC

    ChineseAcadSci,NEInstGeog&Agroecol,LabBlackSoilEcol,Harbin150081,PeoplesRChina

    WangGH(reprintauthor),LaTrobeUniv,DeptAgrSci,Bundoora,Vic3086Australia LaTrobeUniv,DeptAgrSci,Bundoora,Vic3086Australiaguanghuawang@hotmail.com Abstract:Applicationofphosphate.solubilizingrill'croorganisms(PSMs,hasbeenreportedtoincreasePuptakeand

    plantgrowth.However.noinformationisavailableregardingtheecologicalconsequencesoftheinoculationwith

    PSMs.Theeffectofinoculationwithphosphate

solubilizingfungal(PSF)isolatesAspergillusnigerP39andPenicil

    liumoxalicumP66onthebacterialcommunitiesintherhizospheresofmaize(ZeamaysL.'Haiyu6')andsoyl:lean

    rGlycinemaxMerr.'Heinong35'1wasexaminedusingculture

    dependentmethodsaswel1asacultureindependent

    method,polymerasechainreactiondenaturinggradientgelelectrophoresis(PCR

    DGGE).Comparedwiththecontrol,

    thenulnberofculturablemicrobesforsoybeanwassignificantlygreaterwithP39,whereasformaize,thesamewas

    

    29

    significantlygreaterwithP66.Inaddition,agreaternumberofmicrobeswerefoundintherhizosphereofmaize

    comparedwithsoybean.ThefingerprintofDGGEfor16SrDNAindicatedthatinoculationwithPSFalsoincreased

    bacterialcomanunities.withtheP66treatmenthavinghighernumbersofDGGEbandsandahigherShannon

    ,veaverdiversityindexcomparedwithP39;thecompositionofthemicrobialcommunitywasalsomorecomplex

    withtheP66treatment.Overall,complexinteractionsbetweenplantspeciesandexoticPSMsaffectedthestructure

    ofthebacterialcommunityi13_therhizosphere.butplantspeciesweremoreimportantindeterminingthebacterial

    communitystructurethantheintroductionofexoticmicroorganisms.

    Keywords:bacterialcommunity;diversity;PCR--DGGE;phosphate??solubilizingfungus;rhizosphere

    16SRIB0S0MAL

    RNA:MICR0BIALC0MMUNITIES;CLUSTERROOTS;LUPINUS

    ALBUS:WHITELUPIN:

SOIL;NUTRITION:GR0WTH:PLANTS;PCR

    PEDOSPHERE2007,17(2):191199

    Combinedeffectsofcadmiumandbutachloronsoilenzymeactivitiesand

    microbialcommunitystructure

    WangJH,LuYT,ShenGQ,

    ShanghaiJiaoTongUniv,SchAgr&Biol,Shanghai20110l,PeoplesRChina ShanghaiJiaoTongUniv,SchEnvironmSci&Engn,Shanghai200240,PeoplesRChina

    LuYT(reprintauthor).ShanghaiJiaoTongUniv,SchAgr&Biol,2678QixinRd,Shanghai201101,PeoplesRChina

    wangjinhua2@sohucom,ytlu@sjtu.edu.cn,gqsh@sjtu.edu.cn

    Abstract:Thecombinedeffectsofcadmium(Cd,10mkgofsoil)andbutachlor(5,10and50mg/kgofsoil)on

    enzymeactivitiesandmicrobialcommunitystructurewereassessedinphaeozemsoil.Theresultshowedthat

    phosphataseactivitiesweredecreasedinsoilswithCd(10mg/kgofsoil)alonewhereasureaseacitivitieswere

    unaffectedbyCd.Ureaseandphosphataseactivitiesweresignificantlyreducedbyhighbutachlorconcentration(50

    mg/kgofsoil).WhenCdandbutachlorconcentrationsinsoilswereaddedatmilligramratioof2:1or1:2,ureaseand

    phosphataseactivitiesweredecreased.whileenzymeactivitiesweregreatlyimprovedattheratioof1:5.111isstudy

    indicatesthatthecombinedeffectsofCdandbutachloronsoilureaseandphosphataseactivitiesdependlargelyon

    theadditionconcentrationratiostosoils.TherandomamplifiedpolymorphicDNA(RAPD)analysisshowedthatthe

    changesoccurringinRAPDprofilesofdifferenttreatedsamplesincludedvariationinlOSSofnormalbandsand

    appearanceofnewbandscomparedwiththecontrolsoil.TheRAPDfingerprintsshowedsubs

tantialdifferences

    betweenthecontrolandtreatedsoilsamples.withapparentchangesinthenumberandsizeofamplifiedDNA

    fragments.TheresultsshowedthattheadditionofhighconcentrationbutachlorandthecombinedappliedCdand

    butachlorsignificantlyaffectedthediversityofmicrobialcommunity.ThepresentresultssuggestthatRAPDanaly

    sisinconjunctionwithotherbiomarkerssuchassoilenzymeparameteretc.wouldproveapowerfulecotoxicological

    too1.

    Keywords:cadmium(Cd);butachlor;urase;phosphatase;combinedeffect;DNApolymorphismchange

    AMPLIFIEDPOLYM0RPHICDNA:POLYCYCLICAR0MATIC_HYDR0CARB0NS;

    HEAVYMETALS;

    MICR00RGNISMS:MARKERS:MECHANISMS:DIVERSIT;POLLUT10N;PRIME

    RS;BIOMASS

    ENVIRONMENTALGEOLOGY2007,51(7):1221-1228

    

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