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Objective Questions Figure 61 1) In Figure 61, which line best

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Objective Questions Figure 61 1) In Figure 61, which line best

    Microbiology, 9e (Tortora)

    Chapter 6

    Objective Questions

    Figure 6.1

    1) In Figure 6.1, which line best depicts a facultative anaerobe in the absence of O? 2

    A) a

    B) b

    C) c

    2) In Figure 6.1, which line best illustrates a mesophile at 5?C above its optimum temperature?

    A) a

    B) b

    C) c

    3) In Figure 6.1 ,which line best depicts an obligate anaerobe in the presence of O? 2

    A) a

    B) b

    C) c

    4) In Figure 6.1, which line best depicts a mesophile with an optimum temperature of 35?C;

    incubated at 40?C?

    A) a

    B) b

    C) c

    5) In Figure 6.1, which line shows the growth of an obligate aerobe incubated anaerobically?

    A) a

    B) b

    C) c

    6) In Figure 6.1, which line best illustrates the growth of a facultative anaerobe incubated aerobically?

    A) a

    B) b

    C) c

    Answer: A

    Skill: Understanding

7) In Figure 6.1, which line best depicts a catalase-negative cell incubated aerobically?

    Microbiology, 9e (Tortora)

    Chapter 6

    A) a

    B) b

    C) c

Micrococci are facultative halophiles. In Figure 6.1, which line best depicts the growth of M. luteus in a 8)

    nutrient medium containing 7.5% NaCl?

    A) a

    B) b

    C) c

9) In Figure 6.1, which line best depicts a psychrophile incubated at room temperature?

    A) a

    B) b

    C) c

    10) In Figure 6.1, which line best depicts a psychrotroph incubated at 0?C?

    A) a

    B) b

    C) c

    In Figure 6.1, which line best depicts Neisseria gonorrhoeae when growing inside the human body? 11)

    A) a

    B) b

    C) c

    Microbiology, 9e (Tortora)

    Chapter 6

    12) The addition of which of the following to a culture medium will neutralize acids?

    A) Buffers

    B) Sugars

    C) pH

    D) Heat

    E) Carbon

    13) Salts and sugars work to preserve foods by creating a

    A) Depletion of nutrients.

    B) Hypotonic environment.

    C) Lower osmotic pressure.

    D) Hypertonic environment.

    E) Lower pH.

    14) The term facultative anaerobe refers to an organism that

    A) Doesn't use oxygen but tolerates it. B) Is killed by oxygen.

    C) Uses oxygen or grows without oxygen. D) Requires less oxygen than is present in air. E) Prefers to grow without oxygen. Which of the following is not a disadvantage of the standard plate count? 15)

    A) Cells may form aggregates.

    B) Requires incubation time.

    C) Determines viable cells.

    D) Chemical and physical requirements are determined by media and incubation.

    E) None of the above.

    Which of the following is not a disadvantage of the direct microscopic count? 16)

    A) Some organisms are motile.

    B) Enumerates dead cells.

    C) No incubation time.

    D) Sample volume is unknown.

    E) Large number of cells is required.

    Microbiology, 9e (Tortora)

    Chapter 6

Which of the following is not used to determine metabolic activity? 17)

    A) Acid production from fermentation.

    B) CO produced from the Krebs cycle. 2

    -C) NO produced from the electron transport chain. 2

    D) Decreased dissolved oxygen.

    E) Turbidity.

    18) Thirty-six colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard plate count. How

    many cells were in the original sample?

    A) 4

    B) 9

    C) 18

    D) 36

    E) 72

    Figure 6.2

19) In Figure 6.2, which section shows a growth phase where the number of cells dying equals the number of cells

    dividing?

    A) a

    B) b

    C) c

    D) d

    E) a and c

    Microbiology, 9e (Tortora)

    Chapter 6

20) In Figure 6.2, which sections of the graph illustrate a logarithmic change in cell numbers?

    A) a and c

    B) b and d

    C) a and b

    D) c and d

    E) b only

    21) Most bacteria grow best at pH

    A) 1.

    B) 5.

    C) 7.

    D) 9.

    E) 14.

    Figure 6.3

22) Figure 6.3 shows three containers of water connected by tubes. A selectively permeable membrane divides

    each tube. Solutes are added to each container to give final concentrations of 5% NaCl in a; 10% NaCl in b; and

    5% sucrose in c. When the experiment is first set up, the initial movement of water will be

    A) a to b; b to c; c to a.

    B) a to b; c to b; c to a.

    C) a to c; b to c; c to a.

    D) a to c; c to b; c to a.

    E) b to a; b to c; c to a.

    Microbiology, 9e (Tortora)

    Chapter 6

    A culture medium on which only gram-positive organisms grow and a yellow halo surrounds Staphylococcus 23)

    aureus colonies is called a(n)

    A) Selective medium.

    B) Differential medium.

    C) Enrichment culture.

    D) A and B.

    E) B and C.

    24) A culture medium consisting of agar, human blood, and beef heart is a

    A) Chemically defined medium.

    B) Complex medium.

    C) Selective medium.

    D) Differential medium.

    E) Reducing medium.

    25) Which of the following pairs is mismatched?

    A) Psychrotroph growth at 0?C

    B) Thermophile growth at 37?C

    C) Mesophile growth at 25?C

    D) Psychrophile growth at 15?C

    E) None of the above

26) During which growth phase will gram-positive bacteria be most susceptible to penicillin?

    A) Lag phase

    B) Log phase

    C) Death phase

    D) Stationary phase

    E) The culture is equally susceptible during all phases.

    27) Which of the following is the best definition of generation time?

    A) The length of time it takes for lag phase.

    B) The length of time it takes for a cell to divide.

    C) The minimum rate of doubling.

    D) The duration of log phase.

    E) The time it takes for nuclear division.

    Microbiology, 9e (Tortora)

    Chapter 6

    All of the following are direct methods to measure microbial growth except 28)

    A) Direct microscopic count.

    B) Standard plate count.

    C) Filtration.

    D) Metabolic activity.

    E) MPN.

    29) Which group of microorganisms is most likely to spoil a freshwater trout preserved with salt?

    A) Psychrophiles

    B) Halophiles

    C) Anaerobes

    D) Thermophiles

    E) None of the above

    30) Which of the following is an organic growth factor?

    A) Glucose

    + B) NAD

    C) Peptone

    D) NHHPO424

    E) All of the above

    31) Which of the following is an example of a metabolic activity that could be used to measure microbial growth?

    A) Standard plate count

    B) Glucose consumption

    C) Direct microscopic count

    D) Turbidity

    E) MPN

    32) An experiment began with 4 cells and ended with 128 cells. How many generations did the cells go through?

    A) 64

    B) 32

    C) 6

    D) 5

    E) 4

    Microbiology, 9e (Tortora)

    Chapter 6

33) Three cells with generation times of 30 minutes are inoculated into a culture medium. How many cells are

    there after 5 hours?

    10 A) 3 × 2

    B) 1024

    C) 243

    D) 48

    E) 16

34) Which organism produces catalase and superoxide dismutase?

    A) Aerobe

    B) Aerotolerant anaerobe

    C) Obligate anaerobe 35) Which organism is killed by atmospheric O? 2

    A) Aerobe

    B) Aerotolerant anaerobe

    C) Obligate anaerobe

    Which of the following should not be included in a medium used to select for a nitrogen-fixing 36)

    chemoheterotroph?

    A) KHPO24

    B) (NH)SO424

    C) Glucose

    D) MgSO4

    E) NaHPO24

    Microbiology, 9e (Tortora)

    Chapter 6

    Table 6.1

    Below are three different culture media.

    Medium A Medium B Medium C

    NaHPOTide detergent Glucose 24

    KHPONaHPO24 24 Peptone

    MgSOKHPO(NH)SO4 24 424

    CaClMgSOKHPO2 4 22

    NaHCO(NH)SONaHPO3 424 24

37) In Table 6.1, which medium (media) is (are) chemically defined?

    A) A

    B) B

    C) A and B

    D) A and C

    E) None of the above

38) In Table, 6.1, in which medium would an autotroph grow?

    A) A

    B) B

    C) A and B

    D) A and C

    E) None of the above

39) Assume you inoculated 100 cells into 100 ml nutrient broth. You then inoculated 100 cells of the same species

    into 200 ml nutrient broth. After incubation for 24 hr, you should have

    A) More cells in the 100 ml.

    B) More cells in the 200 ml.

    C) The same number of cells in both. 40) The source of nutrients in nutrient agar is

    A) Agar.

    B) Nutrient.

    C) Peptone and beef extract.

    D) Peptone and NaCl.

    E) All of the above.

    Microbiology, 9e (Tortora)

    Chapter 6

    --+41) Which enzyme catalyzes the reaction: O + O + 2H ? HO + O? 22222

    A) Catalase

    B) Oxidase

    C) Peroxidase

    D) Superoxide dismutase E) None of the above

    42) Which enzyme catalyzes the reaction: 2HO ? 2HO + O? 2222A) Catalase

    B) Oxidase

    C) Peroxidase

    D) Superoxide dismutase E) None of the above

    +43) Which enzyme catalyzes the reaction: HO + 2H ? 2HO? 222A) Catalase

    B) Oxidase

    C) Peroxidase

    D) Superoxide dismutase E) None of the above

    Table 6.2

    The following data show growth of two bacteria on different media.

     Amount of Growth

    Staphylococcus aureus Streptococcus pyogenes

    Nutrient agar ++ ++

    Nutrient agar + 7.5%

    NaCl + -

The data in Table 6.2 indicate that S. aureus is a(n) 44)

    A) Mesophile.

    B) Facultative anaerobe. C) Facultative halophile. D) Aerobe.

    E) Halophile.

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