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Proteins pI

By Sandra Green,2014-09-07 05:16
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Proteins pI

    This finding could account for the peculiar behavior of the protein in isoelectric focusing;

    it shows an isoelectric point of 7.4 when the starting pH is 7.0, whereas it shows two isoelectric points, 5.3 and 7.4, when the starting pH is 5.5. Ca2+-dependent change of the fluorescence of the complex of CRP and ANS was also detected. These results suggest a pH- and Ca2+-dependent conformational change of CRP. pI at pH 6.3 ? 0.2.

CRP_HUMAN (P02741)

    Number of amino acids: 224

Molecular weight: 25038.5

Theoretical pI: 5.45

CRP-AE P02741:

ID CRP_HUMAN; STANDARD; 2DG.

    AC P02741;

    DT 01-AUG-1993, integrated into SWISS-2DPAGE (release 0). DT 01-DEC-2000, 2D annotation version 1.

    DT 31-JAN-2008, general annotation version 9.

    DE C-reactive protein [Contains: C-reactive protein(1-205)]. GN Name=CRP; Synonyms=PTX1;

    OS Homo sapiens (Human).

    OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;

    OC Hominidae; Homo.

    OX NCBI_TaxID=9606;

    MT PLASMA_HUMAN.

    IM PLASMA_HUMAN.

    RN [1]

    RP MAPPING ON GEL.

    RA Anderson N.L.;

    RL Personal communication (1993).

    CC -!- SUBUNIT: HOMOPENTAMER

    2D -!- MASTER: PLASMA_HUMAN;

    2D -!- PI/MW: SPOT 2D-0005EI=5.12/23760;

    2D -!- NORMAL LEVEL: 2-5 MG/L [1].

    2D -!- PATHOLOGICAL LEVEL: INCREASED DURING ACUTE AND CHRONIC BACTERIAL

    2D INFECTIONS, CELL NECROSIS, CHRONIC RENAL FAILURE, HODGKINS

DISEASE AND

    2D ACTIVE RHEUMATOID ARTHRITIS [1].

    2D -!- MAPPING: IMMUNOBLOTTING [1].

    CC

    ---------------------------------------------------------------------------

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    CC There are no restrictions on its use by non-profit institutions as long as

    CC its content is in no way modified and this statement is not removed. CC Usage by and for commercial entities requires a license agreement (See

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    ---------------------------------------------------------------------------

    DR DOSAC-COBS-2DPAGE; P02741; CRP_HUMAN.

    DR UniProtKB/Swiss-Prot; P02741; CRP_HUMAN.

    Carbonic anhydrase 2 (EC 4.2.1.1) (Carbonic anhydrase II) (CA-II) (Carbonate dehydratase II) (Carbonic anhydrase C) (CAC) Homo sapiens (Human).

    The computation has been carried out on the complete sequence (260 amino acids).

    Molecular weight: 29246.0

    Theoretical pI: 6.87

ProtParam

    CAH2_HUMAN ( acess number P00918)

    Carbonic anhydrase 2 (EC 4.2.1.1) (Carbonic anhydrase II) (CA-II) (Carbonate dehydratase II) (Carbonic anhydrase C) (CAC) Homo sapiens (Human).

    The computation has been carried out on the complete sequence (260 amino acids).

    Warning: All computation results shown below do not take into account any annotated post-translational modification.

References and documentation are available.

    Please note the modified algorithm for extinction coefficient.

------------------------------------------------------------------

    --------------

    Number of amino acids: 260

Molecular weight: 29246.0

Theoretical pI: 6.87

Amino acid composition: Ala (A) 13 5.0%

    Arg (R) 7 2.7%

    Asn (N) 10 3.8%

    Asp (D) 19 7.3%

    Cys (C) 1 0.4%

    Gln (Q) 11 4.2%

    Glu (E) 13 5.0%

    Gly (G) 22 8.5%

    His (H) 12 4.6%

    Ile (I) 9 3.5%

    Leu (L) 26 10.0%

    Lys (K) 24 9.2%

    Met (M) 2 0.8%

    Phe (F) 12 4.6%

    Pro (P) 17 6.5%

    Ser (S) 18 6.9%

    Thr (T) 12 4.6%

    Trp (W) 7 2.7%

    Tyr (Y) 8 3.1%

    Val (V) 17 6.5%

    Pyl (O) 0 0.0%

    Sec (U) 0 0.0%

     (B) 0 0.0%

     (Z) 0 0.0%

     (X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 32

    Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon C 1329

    Hydrogen H 2027

    Nitrogen N 357

    Oxygen O 384

    Sulfur S 3

Formula: C1329H2027N357O384S3

    Total number of atoms: 4100

Extinction coefficients:

    Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 50420

    Abs 0.1% (=1 g/l) 1.724, assuming ALL Cys residues appear as half cystines

Ext. coefficient 50420

    Abs 0.1% (=1 g/l) 1.724, assuming NO Cys residues appear as half cystines

Estimated half-life:

    The N-terminal of the sequence considered is M (Met).

    The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

     >20 hours (yeast, in vivo).

     >10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 21.68

    This classifies the protein as stable.

Aliphatic index: 76.46

Grand average of hydropathicity (GRAVY): -0.579

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