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methionine synthase and knocking out of cystathionine-beta synthase

By Scott Gordon,2014-12-15 03:19
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methionine synthase and knocking out of cystathionine-beta synthase

    methionine synthase and knocking out of

    cystathionine-beta synthase

    Molecularbiologicalandbiochemicalstudiesrevealnewpathways

    importantforcottonfiberdevelopment

    Xu,YLi,HBZhu,YX

    Pekingun.NatlLabProtEngn&PlantGenetEngn,Beijingl00871,PeoplesRChina. Zhu,YX,PekingUniv,NailI,abPrOtEngn&PlantGenetEngn,Beijingl00871,PeoplesRChinazhuyx@waterpkueducn

    Abstract:Asoneoftilelongestsingle

    celledseedtrichomes,fibersprovideanexcellentmodelforstndyingfunda

    mentalbiologicalprocessessnchascelldifferentiationcellexpansion,andcellwallbiosynthesis.Illthisreview,we

    summarizerecentprogressincottonfunctionalgenomicstndiesthatcharacterizethedynalnicchanges1nthe

    transcriptomesoffibercells.Extensiveexpressionprofilingsofcottonfibertranscriptolneshaveprovidedcolnpre

    hensiveinforlnation.asqniteanumberoftranscriptionfactorsandenzylne

    codinggeneshavebcellshownto

    expresspreferentiallydnringtilefiberelongationperiod.Biosynthesisoftheplanthornloneethyleneisfoundsignifi

    cantlyupregulateddnringtilefibergrowthperiodasrevealedbybothmicroarrayanalysisandbybiochernicaland

    physiologicalstudies,Itissuggestedthatgeneticengineeringoftheethylenepathwaymayimprovetilequalityand

    theproductivofcottonlint.Manymetabolicpathways.suchasbiosynthesisofcelhlloseandmatrixpolysaccha

    ridesarepreferentiallyexpressedinactivelygrowingfibercells.Fivegenefamilies,includin

gprolinerichproteins

    (PRP),arabmogalactanproteins(AGP),expansins,tubulinsandlipidtransferproteins(VYP)areactivatedduring

    earlyfiberde,,elopmcnt.indicatingthattheymayalsobeneededforcellelongation,Inconclusion,weidentifyafew

    areasoffutureresearchforcottonftlnctionalgenonlicstudies. Keywords:cottonfiber;flmctionalgenomics;planthormone;transcriptome;transcriptionfactors

    GOSSYPIUM.HIRSUTUMI.:C'ELLEL0NGAT10N:EXPRESS10NPAFTERNS:GENEEXPRESSION;

    CLONING:IDENTIFICAr10N:1"RANSCRIPTOME:INITIATION:GEN0MICS:DEFINES

    JOURNA0FINTEGRATIVEPLANTBIOL0GY2007,49f1):6974

    AsynergisticeffectontheproductionofS-adenosylLmethioninein

    PichiapastorisbyknockinginofS-adenosyl?-L?-methioninesynthaseand knockingoutofcystathioninebetasynthase

    He,JYDellg,JJZheng,YHGu,J

    PekingUnivNailKeyLabProtEngn&PlantGeneEngn,LSC,Beijing10087I,PeoplesRChina.

    I'!uazhongAgrUnix',Nat[KeyLabAgrMicrobio[,Wuhan430070,PeoplesRChina, Beijing[nstBiomed,Beijing10009I,PeoplesRChina

    Gu,J,PekingUniv,NailKeyLabProtEngn&PlantGeneEngn,LSC,Beijing10087I,PeoplesRChinapkueduell

    Abstracl:Twostrategiesofmetabolicengineeringhavebeenused,individuallyorcombined,toalterthemetabolic

    flnxtoimprovetheproductionofSadenosylL

    methioninefSAM1inPichiapastoris.OneisoverexpressingSAM

    synthasebyknockintechnique,theotheristhedisruptionofcystathionine

    Pssmthase(CBS)byMlockoutteclmique.

StrainGsam?

    rithectopicSAMsynthasegeneprodnced20timesofSAMcomparingtothestarterstrainGS115.

    DisruptionofCBSinGS115onlydonbleditsSAMproduction,However,disrnptionofCBSinGsamresultsina

    robustincreaseofSAMproduction,morethan56times

    synergisticeffectOlltheproductionofSAMinyeastby

    ofthestrainGS115,Thus,wereportforthefirsttimea

    thecombinationofknockinandknockouttechniques,

    Furthermore,weoptimizethecultura1conditionsforthegeneticallymodifiedstrainGsan1cbstoproduceSAM.The

    maximumyieldofSAMreaches3.6g/Li11shakeflaskand13.5g/Li11a5Lfermentor.indicatingthatitcouldbe

    usedforindustria1fennentationtoproduce1argescaleofSAM.

    Keywords:SadenosylInlethionine;geneknock

    outandknockin;metabolicengineering;Pichiapastoris

    SACCHAROMYCESCEREVISIAE:ENZYMrIC

    SYNTHESIS:ADENOSYLMETHIONINE:YEAST;

    TRANSMETHYLATION;EI'HIONINE:G}!NE

    JOURNALOFBIOI~'CtlNOL0GY2006.126(4):519527

    ProgressofvitaminEmetabolicengineeringinplants

    Chen,SYLi,HJLiu,GS

    ChineseAcadSci,KeyLabPhotosynth&EnvironmMolPhysiol,Beijing100093,PeoplesRChina.

    ChineseAcadAgrSci.NatlKeyFacilCropGeneResources&Genetlmprov.NFCRI,Beijing100081,PeoplesRChina

    Liu,GS,ChineseAcadSci,Key1.abPhotosynth&EnvironmMolPhysiol,Beijing100093,PeoplesRChina.1iugs@Ncas.accn

    Abstract:VitaminEisimportantforhulnallandallilna1health.Manyhumandiseases.suchas

certaincancersand

    neurodegenerativeandcardiovasculardisease,areassociatedwiththeinsUmcientintakeofvitalninE.Thedaily

    requirementsforvitanfinEi11111enandwolrlenhavebeenincreasedto15

    30mg.Becausetheprimarysourceof

    dietaryvitaminEcomeSfrOlllplants,thereisaneedtoincreasevitaminEproductionthroughplantengineeringin

    orderto1heetthedemandi11humanconsulnptiol1.Ntlmerousstudieshavebeencarriedoutinthisfield.1eadingto

    manysuccessfu1examples.IllthisreviewwesunmlarizedtherecentprogressinvitaminEmetabolicengineeringin

    plantsaimedatimprovin~thevitalninEcontentandregulatingcompositionofvitaminE. Keywords:vitaminE:plants;metabolicengineering

    SYNECHOCYSTISSPPCC

    6803:PHYDROXYPHENYLPYRUVATEDIOXYGENASE;TOCOPHEROL

    BIOSYNTHESIS:HOMOGENTISATEPHYTYI.TRANSFERASE:4.HYDROXYPH

    ENYIPYRUVATE

    DIOXYGENASE;GERANYLGERANYLREDUC?

    fASE;PLASTOOUnONESYNTHESIS;TRANSCRIPTION

    FlACTORS:SPACHCttL0R0PIASTS;ARABIDOPSISMUTANT

    TRANSGENICRESEARCII2006,15(6):655665

    Inactivationofaldehydedehydr0genase:Akeyfactorforengineering1,3

    propanediolproductionbyKlebsiellapneumoniae

    Zhang,YP",YDu,CYLiu,MCao,Z'

    TsingHuaUnix.,DeptCI~emEngn.1nstBiochenlEngn,Beijingt00084,PeoplesRChina. ChineseAcadSci,InstMicrobiol,Beijing100080,PeoplesRChina.

    Cao,Z,TsingHuaUniv,DeptChemEngn,lnstBiochemEngn,Beijing100084,PeoplesRChinacza<tce@tsinghbua.edu.cn

    Abstract:Productionof1,3-propanediol(1,3

    PD)fromglycerolbyKlebsiellapneunloniaeisrestrainedbyethanol

formation.Thefirststepintheformationofethanollyon1acetyl

CoAiscatalyzedbyaldehydedehydrogenase

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