Buffer Solution
1. Aminoacetic acid –HCl Buffer？0.05M？
X ml 0.2M Aminoacetic acid +Y ml 0.2M HCl , then adding water to reach 200 ml in total volume. pH X/ml Y/ml pH X/ml Y/ml
2.2 50 44.0 3.0 50 11.4
2.4 50 32.4 3.2 50 8.2
2.6 50 24.2 3.4 50 6.4
2.8 50 16.8 3.6 50 5.0
Molecule Weight of Aminoacetic acid ！75.07, 0.2M Aminoacetic acid solution containing 15.01g/L
2. Phthalic acid –HCl Buffer？0.05M？
X ml 0.2M Phthalic acid +Y ml 0.2MHCl , then adding water to reach 200 ml in total volume. pH X Y pH X Y
2.2 5 4.670 3.2 5 1.470
2.4 5 3.960 3.4 5 0.990
2.6 5 3.295 3.6 5 0.597
2.8 5 2.642 3.8 5 0.263
3.0 5 2.032
Molecule Weight of Phthalic acid ！166.13, 0.2M Phthalic acid solution containg 40.85g/L
3. Disodium phosphate - Citric acid Buffer
pH 0.2M Na2HPO4/ml 0.1M Citric acid /ml pH 0.2M Na2HPO4/ml 0.1M Citric acid /ml
2.2 0.40 19.6 5.2 10.72 9.28
2.4 1.24 18.76 5.4 11.15 8.85
2.6 2.18 17.82 5.6 11.60 8.40
2.8 3.17 16.83 5.8 12.09 7.91
3.0 4.11 15.89 6.0 12.63 7.37
3.2 4.94 15.06 6.2 13.22 6.78
3.4 5.70 14.30 6.4 13.85 6.15
3.6 6.44 13.56 6.6 14.55 5.45
3.8 7.10 12.90 6.8 15.45 4.55
4.0 7.71 12.29 7.0 16.47 3.53
4.2 8.28 11.72 7.2 17.39 2.61
4.4 8.82 11.18 7.4 18.17 1.83
4.6 9.35 10.65 7.6 18.73 1.27
4.8 9.86 10.14 7.8 19.15 0.85
5.0 10.30 9.70 8.0 19.45 0.55
Molecule Weight of Na2HPO4！141.98, 0.2M solution containg 28.40g/L
Molecule Weight of Na2HPO4?2H2O！178.05, 0.2M solution containg 35.61g/L
Molecule Weight of C6H8O7?H2O！210.14, 0.1M solution containg 21.01g/L
4. Citric acid -NaOH-HCl Buffer
pH Na+/M Citric acid C6H8O7?H2O/g NaOH/g HCl/ml Total Volume/L
2.2 0.20 210 84 160 10
3.1 0.20 210 83 116 10
3.3 0.20 210 83 106 10
4.3 0.20 210 83 45 10
5.3 0.35 245 144 68 10
5.8 0.45 285 186 105 10
6.5 0.38 266 156 126 10
Keep in refrigerator.
5. Citric acid -Trisodium citrate Buffer？0.1M？
pH 0.1M Citric acid /ml 0.1M Trisodium citrate/ml pH 0.1M Citric acid /ml 0.1M Trisodium
citrate/ml
3.0 18.6 1.4 5.0 8.2 11.8 3.2 17.2 2.8 5.2 7.3 12.7 3.4 16.0 4.0 5.4 6.4 13.6 3.6 14.9 5.1 5.6 5.5 14.5 3.8 14.0 6.0 5.8 4.7 15.3 4.0 13.1 6.9 6.0 3.8 16.2 4.2 12.3 7.7 6.2 2.8 17.2 4.4 11.4 8.6 6.4 2.0 18.0 4.6 10.3 9.7 6.6 104 18.6
4.8 9.2 10.8
C6H8O7?H2O！210.14, 0.1M containg 21.01g/L Na3C6H5O7?2H2O！294.12, 0.1M containg 29.41g/L
6. Acetic acid -Sodium acetatevBuffer？0.2M？
pH 0.2M NaAc/ml 0.2M HAC/ml pH 0.2M NaAc/ml 0.2M HAC/ml
3.6 0.75 9.25 4.8 5.90 4.10 3.8 1.20 8.80 5.0 7.00 3.00 4.0 1.80 8.20 5.2 7.90 2.10 4.2 2.65 7.35 5.4 8.60 1.40 4.4 3.70 6.30 5.6 9.10 0.90 4.6 4.90 5.10 5.8 9.40 0.60 NaAc！136.09, 0.2M containg 27.22g/L
7. Phosphate Buffer
(1) Disodium phosphate -Monosodium phosphate Buffer？0.2M？ pH 0.2M Na2HPO4/ml 0.2M NaH2PO4/ml pH 0.2M Na2HPO4/ml 0.2M NaH2PO4/ml
5.8 8.0 92.0 7.0 61.0 39.0 5.9 10.0 90.0 7.1 67.0 33.0 6.0 12.3 87.7 7.2 72.0 28.0 6.1 15.0 85.0 7.3 77.0 23.0 6.2 18.5 81.5 7.4 81.0 19.0 6.3 22.5 77.5 7.5 84.0 16.0 6.4 26.5 73.5 7.6 87.0 13.0 6.5 31.5 68.5 7.7 89.5 10.5 6.6 37.5 62.5 7.8 91.5 8.5
6.7 43.5 56.5 7.9 93.0 7.0 6.8 49.5 51.0 8.0 94.7 5.3 6.9 55.0 45.0
Na2HPO4?2H2O！178.05 0.2M containg 35.61g/L Na2HPO4?12H2O！358.22 0.2M containg 71.64g/L NaH2PO4?H2O！138.01 0.2M containg 27.6g/L NaH2PO4?2H2O！156.03 0.2M containg 31.21g/L
(2) Disodium phosphate -Monopotassium phosphate Buffer？1/15M？
pH 1/15M Na2HPO4/ml 1/15M KH2PO4/ml pH 1/15M Na2HPO4/ml 1/15M KH2PO4/ml
4.92 0.10 9.90 7.17 7.00 3.00 5.29 0.50 9.50 7.38 8.00 2.00 5.91 1.00 9.00 7.73 9.00 1.00
6.24 2.00 8.00 8.04 9.50 0.50 6.47 3.00 7.00 8.34 9.75 0.25 6.64 4.00 6.00 8.67 9.90 0.10 6.81 5.00 5.00 9.18 10.00 0 6.98 6.00 4.00
Na2HPO4?2H2O！178.05, 1/15M containg 35.61g/L KH2PO4！136.09, 1/15M containg 9.078g/L
8. Monopotassium phosphate –NaOH Buffer？0.05M？
X ml 0.2M KH2PO4 +Y ml 0.2M NaOH, the adding water to reach 20 ml in total
pH(20?) X/ml Y/ml pH(20?) X/ml Y/ml
5.8 5 0.372 7.0 5 2.963
6.0 5 0.570 7.2 5 3.500
6.2 5 0.860 7.4 5 3.950 6.4 5 1.260 7.6 5 4.280 6.6 5 1.780 7.8 5 4.520 6.8 5 2.365 8.0 5 4.680
9. Barbitone sodium –HCl Buffer？18?？
pH 0.04M Barbitone sodium /ml 0.2M HCl/ml pH 0.04M Barbitone sodium /ml 0.2MHCl/ml
6.8 100 18.4 8.4 100 5.21 7.0 100 17.8 8.6 100 3.82 7.2 100 16.7 8.8 100 2.52 7.4 100 15.3 9.0 100 1.65 7.6 100 13.4 9.2 100 1.13 7.8 100 11.47 9.4 100 0.70