High Performance Liquid Chromatography of Ganoderma lucidum spore powder capsule polysaccharide content of_197

By Evelyn Graham,2014-10-30 19:36
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High Performance Liquid Chromatography of Ganoderma lucidum spore powder capsule polysaccharide content of_197

    High Performance Liquid Chromatography of Ganoderma lucidum spore powder capsule polysaccharide content of

     Abstract Objective To establish the Ganoderma spore powder capsule polysaccharide content in HPLC determination. Methods ShodexSH1011 (styrene-divinylbenzene copolymer

    bonded sulfonic) sugar column, mobile phase of 0.6% sulfuric acid solution, detection wavelength 200 nm. The results of Ganoderma lucidum polysaccharides in the 0.266 ~ 8.51 mg / ml range a good linear relationship, r = 0.999 8, the average recovery was 100.4% (n = 9), RSD = 1.57%. Conclusion The method is simple and quick, non-interference, good

    reproducibility and can be used for Ganoderma spore powder capsule quality control.

     Key words Ganoderma lucidum spore powder of Ganoderma lucidum polysaccharides by high performance liquid chromatography capsule

     Content Determination of Polysaccharides of Ganderma lucidum in Spore Powder Capsule by HPLC

     Abstract: ObjectiveTo establish an HPLC method for determination of Ganoderma lucidum

    Polysaccharide in spore powder capsule. MethodsA Shodex SH1011 column was used with 0.6% sulphuric acid solution. The detection wavelength was set at 200nm.ResultsThe linear relationship of the concentrations and peak areas was good in range of 0.266 ~ 8.51 mg /

    ml (r = 0.999 8). The average recovery was 100.4% (n = 9), RSD = 1.57%. ConclusionThe method is simple, rapid and accurate and can be used for quality control of the capsule.

     Key words: Spore powder of Ganderma lucidum ganderma capsule; Polysaccharides; HPLC

     Ganoderma lucidum spore powder capsule from the Ganoderma spore powder capsule [WS

    5026 (B 0026) 2004] to change formulations made with spleen Qi, Yang Anshen effect. For the Heart and deficiency, sickness and infirmity, as well as cancer patients after adjuvant treatment. The effective ingredients of Ganoderma lucidum polysaccharides, triterpenoids, with its polysaccharide content of about 0.75% [1]. Determination of polysaccharide used sulfuric acid - anthrone [2] and sulfuric acid phenol

    spectrophotometric method [3,4]. The method error larger, more complicated operations. Determination of polysaccharide content using HPLC [5,6] have reported that Ganoderma

lucidum spore powder formulations using high performance liquid chromatography

    polysaccharide not been reported. This selection of high-performance liquid chromatography

    method for the fungus Ganoderma lucidum spore powder soft capsules in the Determination of polysaccharide in the establishment of a high separation efficiency, good stability, rapid analysis method for controlling quality of preparation provided a reliable method. The study methodology, in line with requirements determination.

     An instrument and reagent

     Shimadzu LC 10A high-performance liquid chromatograph, SPD 10Avp UV - visible

    detector, N2000 chromatography data workstations, KQ 500DB CNC ultrasonic cleaner,

    7725i six-way injection valve.

     D anhydrous glucose reference substance (China Pharmaceutical and Biological

    Products Institution, batch number 110833 200302, for the determination of use);

    Ganoderma lucidum spore powder soft capsule (Nanjing Pharmaceutical Co., Ltd., batch number 20050301,20050302,20050303) sulfuric acid AR, water re-distilled water, other

    reagents were analytical pure.

     2 Methods and Results

     2.1 Chromatographic conditions

     Column: ShodexSH1011 (styrene-divinylbenzene copolymer bonded sulfonic) (8.0 mm ×

    300 mm, 6 μm), mobile phase: sulfuric acid water (6:1 000), detection wavelength 200

    nm, flow rate 0.6 ml / min, injection volume of 20 μl, column temperature 30 ?. In the

    above-mentioned chromatographic conditions, D anhydrous glucose retention time of 4.987


     2.2 The product solution and reference substance for the test preparation solution

     This product is 10 to take the contents mixed evenly, taking about 3.0 g, precision that will placed in 10 ml glass centrifuge tube, add n-hexane 8 ml, shock 2 min after

    centrifugation (3000 r / min) 10 min, discard to n-hexane layer, and then repeat the above

    steps once, water bath to shake off residual n-hexane, it will transfer them to the 10 ml

    flask, add the appropriate amount of water, 100 ? water bath 1 h, lack of water, mixing,

    filtration, take filtrate 2.0 ml by adding ethanol 8.0 ml, shake and place 2 min after centrifugation (3 000 r / min) 10 min, supernatant and sediment dissolved with 2.0 ml mobile phase, 1 h after 100 ? water bath that is too . Separate examination of the hydrolysis time. The results in Table 1. Examination of hydrolysis time in Table 1 (omitted)

     The results can be completely hydrolyzed more than 1h, so choose 1h as the hydrolysis point in time.

     Precision said the scheduled 48 h vacuum drying of the D anhydrous glucose

    reference substance 21.28 mg, buy 10 ml flask, add the mobile phase was dissolved and diluted to the scale, shake, made with D per ml anhydrous glucose 2.128 mg of the

    solution, filtration, that is, too.

     Adaptability of the system, an experimental study. The results in Table 2. Table 2 Experimental results of system suitability study (omitted)

     2.3 The linear relationship of the study

     Precision drawing reference substance solution of 5 ml in 10 ml volumetric flask in

    the dilution constant volume, followed by two-fold dilution prepared with the reference

    substance solution, according to the above-mentioned chromatographic conditions, precision drawing 20 μl injection of liquid chromatography, continuous sample 2 times measured. To