High Performance Liquid Chromatography Liver capsule content Schisandrin
【Abstract】 Objective To investigate the allicin on
Hemorheology and physiological characteristics of red blood cells. Method of producing a high viscosity rat model of acute
arterial blood, through the determination of blood viscosity and erythrocyte aggregation index change index, the nuclear pore membrane filtration Determination of erythrocyte filtration index, fluorescence polarization determination of red cell membrane fluidity, as well as red blood cell infiltration fragility test, comparison of allicin intraperitoneal injection group and the untreated group differences. Results with the untreated group, allicin high, medium and low-dose group and blood stasis syndrome in rats
increased whole blood viscosity were significantly lower effect (P <0.01, P <0.05); middle-and high-dose group on
plasma viscosity significantly reduce the effect (P <0.05), and these effects showed a definite dose-effect relationship;
high-dose group could significantly lower hematocrit (P <0.05), can significantly improve the deformability of red blood cells (P <0.05); in low-dose group, to reduce the role
of erythrocyte aggregation; each dose group the increase of osmotic fragility of red blood cells to improve role; each dose group on erythrocyte membrane fluidity reduction to improve the role, but not statistically significant. Conclusion of allicin can improve erythrocyte deformability and lower blood viscosity, improve blood rheology, whereas for
the prevention and treatment of blood stasis syndrome.
Key words allicin; acute blood stasis syndrome; Hemorheology; erythrocyte
Effects of Allicin on Hemorheology and Physiological
Properties of Erythrocytes in Acute Blood-stagnated Rats
Abstract: ObjectiveTo explore the effects of allicin on hemorheology and physiological properties of
erythrocytes.MethodsWe established the rat models of acute hyperviscosity and collected artery blood samples. Then we examined the hemorheology index and erythrocyte aggregation index by the viscosity methods, detected the erythrocyte filtration index by the nuclear pore film, determined erythrocyte membrane fluidity by the fluorescence polarization and performed erythrocyte osmotic fragility test. The
difference between the allicin intrapertoneal injection group and the untreatment group was observed.ResultsIn comparison with the untreatment group, all the high-, medium - and low-
dose groups of allicin treatment had a decline in the enhancement of whole blood viscosity in rats with blood stasis (P <0.01, P <0.05). In the high-and medium-dose groups, the
plasma viscosity significantly decreased (P <0.05) and had the dose-effect relationship. In the high-dose group, the
hematocrit greatly decreased (P <0.05) and the erythrocyte deformability increased (P <0.05). In the medium-and low-dose
groups, the erythrocyte aggregation index decreased. All dose of allicin had effect on the increase of erythrocyte osmotic fragility and the decrease of erythrocyte membrane fluidity,
but no significance was detected. ConclusionAllicin may increase the erythrocyte deformability, decrease the whole blood viscosity and improve the blood rheological properties and it can be used in the treatment of blood stasis.
Key words: Allicin; Acute blood stasis; Erythrocyte; Hemorheology
Allicin (Allicin) has anti-bacterial, anti-fungal, anti-
tumor and the role of pesticides, etc. . In recent years, found that there are anti-virus, enhance immune function,
scavenging free radicals, anti-oxidation, reducing blood
pressure, blood fat, anti-atherosclerosis, lowering blood
sugar, anti-arrhythmia and the role of convergence . At present the medical profession which have been widely used for prevention and treatment of many diseases. Their blood pressure, lipid-lowering, anti-atherosclerotic effect mainly
on hemorheology, while hemorheology studies, most focus on
platelet function, the pairs of red blood cell rheology studies small. This article focuses on studies of allicin on
erythrocyte rheology and other physiological characteristics.
1.1 Animals Wistar rats of either sex, weight (200 ? 20)
g, 3 ~ 4 months of age, purchased from Beijing Haidian Tom Lee Laboratory Animal Breeding Center.
1.2 Drugs and reagents produced in Shanghai Garlic injection Hefong Pharmaceutical Co., Ltd., 30 mg / 2 ml.
1.3 main instruments NXE 1-type cone-plate viscometer:
Chengdu Instrument Factory products. DXC 300 type nuclear
membrane red blood cell deformability analyzer: Shanghai Medical Instrument Factory products. 650 60 Fluorescence
Polarization Instrument: Japanese Hitachi products. Micro-
high-speed centrifuge: Aerospace Huaxing Aircraft Wheel Corporation products.
2.1 Animal Grouping and administration of 50 experimental animals were randomly divided into 5 groups: blank control
group (saline 2 ml * kg-1 * d-1), acute hyperviscosity model
group (saline 2 ml * kg -1 * d-1), allicin low-dose group (5
ml * kg-1 * d-1), the dose of allicin (10 ml * kg-1 * d-1),
allicin high-dose group ( 20 ml * kg-1 * d-1). In addition to
the blank control group, the remaining animals fed a daily tap water 5 ml / only consecutive 7 d, the 7th day subcutaneous injection of 0.1% concentration of adrenaline 0.08 ml/100 g body weight, injected 2 h, immersed in ice water in 5 min an interval of 4 h injection of the first two times, producing acute hyperviscosity model . After modeling of all animals in the first two days starting the daily intraperitoneally (ip) corresponding drugs a second consecutive medication 7 d.
2.2 The changes in blood flow indices and the
determination of viscosity, erythrocyte aggregation method .
2.2.1 Blood and anticoagulation after the last administration 2 h, intraperitoneal injection of pentobarbital
sodium 40 mg / kg of narcotic. Carotid artery blood, heparin
20 IU / ml anticoagulant.
2.2.2 Determination of whole blood viscosity and plasma viscosity to take anti-clotting 1.3 ml, at 37 ?,
respectively, blood samples were measured in the shear rate 230S-1, and 11.5 S-1 when the value of whole blood viscosity.
2000r/min centrifuge 15 min, separated plasma were measured in 115 S-1 when the plasma values.
2.2.3 hematocrit (Hct) determination of Wen's method. Capillary pipette drawn with 0.5 ml of anti-coagulation,
insert the tube at the bottom of Wen's hematocrit, so that
blood to the scale at only 10. Do not inject air bubbles. Blood samples will be injected into the centrifuge tube hematocrit tube placed inside the bucket, to 2 300 r / min centrifugation 30 min. Remove the PCV pipe, in the white blood
cell and red blood cell level of alignment boundaries was read out at the scale of values, that is, the percentage of hematocrit.
2.2.4 erythrocyte aggregation index calculated red blood cell aggregation index (ηre) is defined as the