Hebei Xiangju accumulation and content of total flavonoids determination
【Abstract】 Objective To determine the total flavonoid content Xiangju. Methods macroporous resin concentration, 60% ethanol elution was flavonoids and ultraviolet
spectrophotometry assay. Results easy to operate, with a total flavonoid content of 37.35% on average. Conclusion accumulation rate is high and stable, accurate and reliable determination.
Key words Hebei Xiangju enrichment of total flavonoids
Concentration and Determination of the Flavonoid of Hebei Balmy Chrysanthemum
Abstract: ObjectiveTo determine the flavonoid.
MethodsUsing macroporous resin column chromatogram, to get 60% ethanol fraction. Using UV spectrum to determine the flavonoid
of this part. Results The mean content of the flavonoid was 37.35%. Conclusion The rate of concentration is high and stable, the method of determination is reliable.
Key words: Hebei balmy chrysanthemum; Flavonoid; Concentrate
Medicinal and Edible chrysanthemum as a traditional Chinese medicine, with wind-dispersing heat, Pinggan eyesight,
detoxification, relief of the effectiveness of . Qu Yuan's Li Sao, there is "evening meal Qiuju of Laying" the sentence. Chrysanthemum because of its refreshing taste in Chinese
culture is very popular, widely used in people's everyday health-care tea. Some studies have shown , chrysanthemum volatile oil and soothing, total flavonoids blood pressure, is
its wind-dispersing heat, the material basis for Pinggan
eyesight. In Hebei Province, Hebei Academy of Agricultural Sciences Xiangju cultivated new varieties of medicinal Chrysanthemum, is currently the only Luzhou Chrysanthemum, its volatile oil and total flavonoids were significantly higher than other similar products, nutritional value and medicinal value of health care, are very large, the development prospect is broad. Therefore, this paper, Hebei Xiangju enrichment process of total flavonoids was studied, using UV spectrophotometry conducted a Determination of total
flavonoids. Hebei Xiangju pharmaceutical and nutritional value, providing a scientific basis for the further development and utilization.
1 Materials and methods
UV spectrophotometer (Shimadzu UV-2201); D101 macroporous
resin (Tianjin Friends Chang Industry Co., Ltd.); methanol (AR, Tianjin, Wing Tai Products Corporation); Acacia Su-7-O -
β-D-glucoside-based laboratory-made reference substance
(purity 99.2%); of Hebei Province, Hebei Academy of Agricultural Sciences Xiangju provided by planting drugs.
1.2 method [3,4]
1.2.1 Preparation of reference substance solution Precision Weigh acacetin-7-O-β-D-glucoside reference
substance 5.06 mg, placed in 50 ml flask, add methanol to make it dissolved, diluted to scale shake, derived reference substance solution.
1.2.2 Determination of the wavelength you choose to reference substance solution, 2 ml placed in 25 ml flask, the constant volume, shake. Will join the cuvette in the solution at 200 ~ 800 nm wide wavelength range wavelength scanning, respectively, have 328,269.3,208 nm absorption peak. Measured using the same method for the solution of test items are, respectively, 328,208 nm absorption peak.
1.2.3 Standard Curve drawing precision drawing
1.0,1.5,2.0,2.5,3.0,3.5 ml solution of the above-mentioned
reference substance were placed in 25 ml flask, the constant
volume, shaken, placed in the cuvette, was selected The wavelengths measured absorbance A. Method of least squares regression equation was used, and linear range of determination.
1.2.4 Enrichment of flavonoids called to take a certain quality of dry Hebei Xiangju adding eight times the amount of 70% ethanol, at 80 ?, the thermal reflux 1.5 h, filtered out of solution, another 7-fold by adding the amount of 70%
ethanol, at 80 ? and heat reflux 1.5 h, and properly combined filtrate was concentrated, the above concentrated liquid drops added to the macroporous resin column eluted with distilled water until the outflow of fluid Molish negative reaction, and then 3 000 ml 60% ethanol elution (approximately 10 column
volume), collecting eluate, solvent recovery, and further concentrated to dry paste, which was 70% ethanol extracts by macroporous resin 60% ethanol elution position (60% ethanol for short positions) . Such a system were three groups in this
Weigh the dry paste precision 4.5 mg, made from 25 ml methanol solution, from which accurate drawing 2.0 ml placed in 25 ml flask, using methanol fixed volume, that is, items were tested solution. Reposted elsewhere in the paper for free
1.2.5 Methodology validation
Precision Experiment: Precision drawing reference substance solution of 2.0 ml placed in 25 ml flask in constant volume of methanol. The selected wavelength, repeat five times
the value measured its absorption.
Recovery experiment: precision that has been taking constant weight dry paste the above-mentioned five copies,
each about 4 mg, respectively, by adding 1 mg precision of about reference substance, according to "1.2.4" entry prepared
solution were measured at selected wavelengths absorption values, calculation of the average recovery and RSD.
Repeated experiment: Precision Weigh "1.2.4" Preparation of dried paste item 4.5 mg. Click "1.2.4" entry test preparation materials for the solution of the selected wavelength absorbance measurement repeated five times.
Stability of the experiment: take the same test materials for the solution of 0,2,4,8,12,24,48 h, respectively, measured absorbance.
Sample Determination: Precision Weigh three groups, each consisting of three copies, prepared in accordance with items 1.2.4 location Dry Extract 60% ethanol, a total of nine copies, each of about 4.5 mg, prepared in accordance with 1.2.4 key solution for the test product at the selected
wavelength of absorption values were measured to calculate concentration.
2.1 Determination of Wavelength Selection
Reference substance and test products for a total absorption of 328 nm as detection wavelength.
The standard curve and linear range of 2.2 to reference substance concentration (X, μg / ml) as abscissa, to absorb
the value of (Y) for the vertical as standard, rendering the standard curve, linear regression analysis was the regression
equation Y = 0.039 60 0.049 51X, r = 0.999 2, the linear range of 2.048 ~ 16.192 μg / ml.
2.3 Precision Experiment
Taking a concentration of reference substance solution, five times repeated measurements, RSD value of 0.36%.
2.4 recoveries recoveries experimental results show that Acacia Su-7-O-β-D-glucoside reference substance, the average recovery rate of 98.32%, RSD 0.57%. The results in Table 1. Table 1 The average recovery results (omitted)
2.5 Repeatability test
Reproducible experimental results show that the first three batch dried paste total flavonoids content of 38.10%, RSD was 0.13%.
2.6 Stability Experiment results show that the stability
of the sample solution was stable in the 48 h period, RSD
value of 0.67%.
2.7 Determination of three sample groups 60% ethanol dried paste parts of total flavonoids contents were 36.90%, 37.28%, 37.88%. Average of 37.35%, RSD values were 0.21%, 0.50%, 0.89%. The results in Table 2. Samples in Table 2
Determination of total flavonoids results (omitted)
This paper discusses the 60% ethanol part of the enrichment process of total flavonoids. The results showed that the use of macroporous resin concentration, 60% ethanol eluted 10 column volume, total flavonoids derived from a stable and relatively high accumulation rate, blood pressure effect is remarkable. This method can be widely used; In this paper, UV spectrophotometry of 60% ethanol part of the total flavonoid content, the method of high precision, good
repeatability and measurement results are accurate, this site provide the basis for further development and utilization of research.
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