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Ginkgo biloba extract on rats with chronic ocular retinal injury in rats_2663

By Bradley Bell,2014-10-30 18:40
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Ginkgo biloba extract on rats with chronic ocular retinal injury in rats_2663

    Ginkgo biloba extract on rats with chronic ocular retinal injury in rats

     Abstract Objective: To explore the Ginkgo biloba extract (EGb) on experimental high intraocular pressure in rat retinal ganglion cell layer (RGCL) neuronal injury in rats. Methods:

    SD rats were 60 healthy, normal control group and the normal Ginkgo biloba treatment group 6, the remaining 48 using only burn branding law, branding the left eye closed two shallow scleral rat vein, rats continued to produce high IOP model, to

    choose the level of intraocular pressure and stability required in the experiment 30 rats were randomly divided into normal saline group, the treatment A group (per day EGb 50mg/kg), the treatment B group (per day EGb 100mg/kg) and treatment C (per day EGb 150mg/kg), treatment D (per day EGb 200mg/kg), the treatment time for 1mo, rats were killed after the shop to do full-thickness retina films, to do right RGCL neuron-specific staining OK neuron counts analysis to assess the neuronal situation. Results: The normal control group eyes RGCL neuron counts showed no significant difference (P> 0.05), normal control group and normal EGb treated group RGCL neuron counts showed no significant difference (P> 0.05), various concentrations of EGb experimental treatment group eye with

    the normal control group RGCL counts were significantly difference (P> 0.05), various concentrations of EGb treatment group, experimental eye to its own control eye RGCL neuron counts were significantly different (P> 0.05), various

    concentrations of EGb treatment group, Experimental Eye experiment with the saline group eyes RGCL neuron counts were significantly different (P> 0.05), treatment A, B, C, D 4 set of experimental eyes RGCL neuron counts in 22 there was a significant difference (P < 0.05). Conclusion: The single use of EGb on the neuron counts in normal rats RGCL no effect, EGb may continuous chronic elevated intraocular pressure leading

    to neuronal damage in rat RGCL provide some protection, and the protective effect within a certain range size and dose

    positive related.

     Key Words Ginkgo biloba extract in rat ocular

    neuroprotection

     Protective effect of extract of ginkgo biloba leaves on retinal neuron injury in chronic high intraocular pressure rats

     Abstract AIM: To investigate the protective effect of extract of ginkgo biloba (EGb) on retinal ganglion cell layer neuron injury induced high intraocular pressure (IOP) in rats. METHODS: Sixty healthy Sprague-Dawley (SD) rats were randomly

    divided into 3 groups : 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups : 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg / (kg * d); group B, EGb 100mg / (kg * d) ; group C, EGb 150mg / (kg * d); and group D EGb

    200mg / (kg * d). After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer (RGCL) from both eyes of each rat

    were performed to evaluate neuron situation. RESULTS: There was no significant difference between the bilateral eyes on neuron counting from RGCL of normal control group (P> 0.05). There was no significant difference between the normal control

    group and the EGb treating normal IOP group on neuron counting from RGCL of (P> 0.05). The difference was significant between the experimental eyes of every dose group and normal control group (P <0.05). The difference was significant between the experimental eyes and self-controlled eyes in every dose group

    (P <0.05). The difference was significant between the experimental eyes of every dose group and physiological brine treating group (P <0.05). The difference was significant between the experimental eyes on neuron counting from RGCL of

    every dose group (P <0.05). CONCLUSION: EGb has no effect on neuron counting of RGCL when administered alone in normal rats. EGb could partly provide the protective effect on

retinal ganglion cell layer neuron injury induced chronic high

    IOP in rats. The protective effect of EGb will be enhanced according to the increasing dose in a definite range.

     * KEYWORDS: extract of ginkgo biloba; rats; high intraocular pressure; neuroprotection

     0 Introduction

     Glaucoma is a group made up of many factors, pitting caused by optic nerve atrophy and visual field defect as a common feature of the disease, pathological elevation of intraocular pressure is the most important risk factors. Therefore, most clinical treatment methods to reduce the

    intraocular pressure-based, but the intraocular pressure

    reduction after a lot of the time was still on the progressive damage to the optic nerve, retinal ganglion cell layer neurons will eventually apoptosis, so find a way to protect RGCL nerve

    Yuan drugs, delaying and reducing neuronal apoptosis, the effective protection of the optic nerve, has become the focus of today's glaucoma research and hot. Ginkgo biloba extract (extract of ginkgo biloba leaves, EGb) from the plant Ginkgo biloba extract crafted of pure natural medicine. The main ingredient for the flavonoids and terpene lactones, also contains organic acids, polysaccharides, polyphenols, amino acids, polyprenols, such as steroid and trace elements. In recent years, many studies have proven that EGb has a clear

    free radicals, inhibiting platelet activating factor, inhibition of nitric oxide synthase to protect mitochondria, inhibit glutamate toxicity and other physiological functions [1,2], on this basis, our research EGb could delay or reduce

    the persistent chronic elevated intraocular pressure on rat retinal ganglion cell layer (RGCL) neuronal damage.

     1 Materials and methods

     1.1 Material from the Experimental Animal Department of Nanhua University to provide health Sprague-Dawlay rats 60,

    female, body weight 200 ~ 240g, 8 ~ 12wk age, no significant distortion upon inspection neck, transparent cornea, iris blood vessels clear, pupils and other big circle, etc. , light responsiveness. 3d experimental feeding, the continuous

    measurement of intraocular pressure 3d, excluding the average intraocular pressure higher or lower fluctuations in rats with

    normal-tension range (8 ~ 20mmHg) by [3], to single out qualifier included in the experiment. Ginkgo biloba capsules (39 Pharmaceutical Yellowstone pharmaceutical, 0.15g / tablets, containing Ginkgo biloba extract 40mg / tablets).

     1.2 Methods six rats were randomly selected as normal control group, six of EGb treated normal group, the rest are used siu branded method [3], branded a closed left eye 2 in

    rat superficial scleral veins, producing sustained high rat IOP model, the right eye is not an operation, as self-control,

    observation 1wk, IOP stabilized at 26mmHg in the left eye for more than 30 randomly selected into the experiment, and then

    were randomly divided into normal saline group, the treatment A group (daily Egb 50mg/kg), the treatment B group (per day EGb 100mg/kg), treatment C (per day EGb 150mg/kg), treatment D (per day EGb 200mg/kg), each 6 to carry out gavage treatment

    1mo, once daily morning dose to the whole. Weekly monitoring of intraocular pressure 1. Taken after the expiry of each set of experiments for full-thickness retina chip shop, cresol

    violet staining Across RGCL solid neuron counts. Shop the retina under test each piece symmetrical drawing a line through the optic nerve will be divided into temporal, temporal, the nose, the nose four quadrants, and the retina is divided into three equal portions of each quadrant, that is the central, middle and around three areas, each district were randomly taken three points, each point of an area of 32 500μ

    m2, then every point of the retinal neurons counted, and then converted into the number of cells per square millimeter (cells / mm2), each Area 3 points of the mean cell density of

    neurons as the density of the area. By morphology (Figure 1) easy to identify RGCL in vascular endothelial cells, with dense nuclear staining and no Nissl substance cells glial cells, which are not included in the two cell RGCL neuron

    counts.

     Statistical analysis: All data are in the WindowsXP environment, the use of statistical software SPSS11.0 processing. Statistical methods using single-factor analysis

    of variance (One-Way ANOVA) and t test significance level of P <0.05.

     Figure 1 stretched temporal retinal quadrant (Cresyl violet solid × 400) A: treatment A group; B: Treatment of B group; C: Treatment of C group; D: Treatment of D group

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     2 Results

     SD rats with normal right eye and left eye of RGCL neuron counts were (3483.3 ? 21.5) a / mm2 and (3491.2 ? 11.9) a /

    mm2, eyes, no significant difference (P> 0.05). And from the central zone to peripheral neurons counts gradually reduced. Normal EGb treatment group compared with the normal control group, no significant difference (P> 0.05), various concentrations of EGb treatment group experimental eye and the normal control group RGCL neuron counts were significantly different (P

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