Ginkgo biloba extract on focal cerebral ischemia in rats
【Abstract】 Objective To observe the Ginkgo biloba extract (GBE) on focal cerebral ischemia in rats, and to explore its mechanism. Methods suture middle cerebral artery occlusion in
rat focal cerebral ischemia model building to observe the GBE on the symptoms of nerve injury rat model, cerebral infarction area and serum SOD activity, MDA content of impact. Results GBE could significantly improve the neurological symptoms of focal cerebral ischemia in rats, reducing the scope of cerebral infarction and serum MDA, serum SOD activity increased. Conclusion GBE on focal cerebral ischemia has a protective effect, the mechanism may be related to antioxidant effects.
【Key Words】 Ginkgo biloba extract; rats; focal cerebral ischemia; infarct size; lipid peroxidation
Effect of Ginkgo biloba Extract on Cerebral Ischemia-
reperfusion Injury in Rats
WANG Guang ming
Guangdong Chemical and Pharmaceutical Professional
College, Guangzhou 510520, China
Abstract: ObjectiveTo observe the effect of Ginkgo biloba extract (GBE) on cerebral ischemia injury in rats and explore its mechanism.MethodsRat cerebral ischemia injury model was induced by middle cerebral artery occlusion, the nerve injury
symptoms were evaluated, the infarction area and the level of SOD and MDA in serum wrer determined.ResultsGBE could improve
the nerve injury symptoms, reduce the infraction area of brain and the level of MDA, increase the activity of
SOD.ConclusionGBE has protective effect on cerebral ischemia injury in rats and its mechanism may be associated with antioxidant activity.
Key words: Ginkgo biloba; Rats; Cerebral ischemia injury; Infarction area; Lipid preoxidation
Division of Ginkgo biloba Ginkgo biloba Ginkgo biloba Ginkgo biloba leaf plants, sexual bitter, astringent, flat, the main function of promoting blood circulation Huayu, Tung network rolling. At present, the extract (Ginkgo biloba extract, GBE) is widely used to treat cardiovascular and
cerebrovascular disease and its sequela. Studies have shown that, GBE's main active ingredients of flavonoid glycosides and terpene lactones . In this study, suture middle cerebral artery occlusion in rat focal cerebral ischemia model
building to observe the GBE on focal cerebral ischemia in rats and to explore its mechanism, in order to provide clinical cerebrovascular disease prevention and treatment reference.
1.1 Animals Male SD rats weighing (200 ? 20) g, by the
Guangdong Medical Science Institute. Adaptive feeding 1 week before the experiment.
1.2 Drugs and reagents of Ginkgo biloba extract (trade name: Tian Bao Ning films), Zhejiang CONBA Group Pharmaceutical Co., Ltd., Spec 40 mg (which contains Ginkgo
flavone glycosides 9.8 mg), lot number 030,124. Nimodipine, Tianjin Central Pharmaceutical Co., Ltd. production, batch number 031.22 thousand. 2,3,5 - triphenyl tetrazolium chloride
(TTC), AR, Shanghai Pharmaceutical Group, Shanghai Chemical
Reagent Company. SOD, MDA kit, Nanjing Jiancheng Bio-
engineering Institute, lot number, respectively 20040128,20040120.
1.3 Instruments Electronic Analytical Balance, Shanghai Jing Section analytical instrument factory. LXJ-IIB low-speed
large-capacity centrifuge, Shanghai Anting Scientific Instrument Factory. 754 UV-Vis spectrophotometer, Shanghai
Analysis Instrument Factory.
2.1 Animal grouping and drug delivery to take 60 rats were randomly divided into six groups, namely, GBE high,
medium and low-dose group (dose of 48,24,12 mg / kg),
nimodipine group (dose of 2 mg / kg), model control group and sham operation group, n = 10. Modeling in each group, respectively, before the administration, a time / d, were administered four times, delivery volume were 10 ml / kg. Model control group and sham-operated group to the same volume
2.2 The modeling approach after the last administration 1 h, the light of literature method [2,3], with 10% chloral hydrate (350 mg / kg) intraperitoneal injection of
anesthetized rats, supine fixed, regular disinfection of the skin, the middle neck incision , blunt separation of the right common carotid artery, external carotid artery and internal carotid artery. Downward along the internal carotid artery
pterygopalatine artery was isolated, and coagulation. Artery clip with carotid artery occlusion in internal carotid artery near the carotid artery down the wing artery was isolated, and coagulation. Artery clip with carotid artery occlusion in
internal carotid artery near bifurcation of common carotid artery cut all the ports, will be a fishing line (diameter 0.30 mm) along the internal carotid artery to the brain slowly into the mouth of the resistance was somewhat stop, insert a length of about (18.0 ? 0.5) mm, ligation of a fixed fishing line. Ligation of internal carotid artery proximal end of the clip removed artery to restore blood flow in the external carotid artery. Sham-operated group except non-inserted
fishing line the middle cerebral artery occlusion, the other operations with the surgical group.
2.3 OUTCOME MEASURES
2.3.1 neurological symptom score reference to literature method [4,5], according to extent of the damage developing neurological symptoms rating scale. No symptoms of nerve
injury grade ?; not fully extend the left forepaw of ?
level; not fully extend the left forepaw, turning in circles to the left of ? level; not fully extend the left forepaw, to the left of the dumping of grade ? ; not spontaneously walk,
loss of consciousness for the grade ?. According to the above
criteria, in modeling after 24 h, single-blind method on rats
in each group to conduct neurological symptom score.
2.3.2 of serum SOD activity and MDA content determination after model 24 h, chloral hydrate anesthetized rats, abdominal aortic blood, centrifugal separation of serum, according to kit methods of operation, colorimetric determination of serum SOD activity and MDA content.
2.3.3 Determination of the scope of decapitated cerebral
infarction, remove odor, cerebellum, saline wash, filter paper exhaust moisture, the brain along the coronal plane slice thickness of 5 mm slices, and placing 1% TTC phosphate buffer solution at 37 ? in dark incubation temperature 10 min, wash Staining, and then 10% neutral formaldehyde solution, fixed 24
h, remove the dye, said the total weight, and infarct area were cut weighed to calculate infarct size. Infarct size = infarct weight × 100% / total Someshige.
2.4 Statistical analysis using ? s, said more than a few
between the two groups were compared using single factor analysis of variance, data statistics using SPSS13.0FOR Windows software processing. Statistical tests used Ridit level. Reposted elsewhere in the paper for free download http://
3.1 the impact of symptoms of nerve injury from the Table 1 we can see that after administration 24 h, GBE high-dose
symptoms of nerve injury in rats significantly improved compared with the model control group, there was significant difference (P <0.05).
Table 1 on the impact of symptoms of nerve injury (abbreviated)
3.2 the impact of infarct size from Table 2 we can see, GBE high and middle dose rats significantly reduced cerebral weight, compared with the model control group, significant differences. GBE range of high-