Garlic injection with several antibiotics Experimental Study of Compatibility
Author: Fu Yu Zhou Yajun spine power Xia Hong XIA Zhi Xue
Key words garlic;, and compatibility;,, stability;,, UV spectrophotometry,
Abstract: Objective: To investigate the garlic injection with ceftriaxone, ornidazole, and other seven kinds of injection in 5% glucose injection in the stability of compatibility. Methods: The observation at room temperature 25 ? Allicin compatibility with the drug after the
appearance, pH, changes, and ultraviolet spectrophotometry allicin, ceftriaxone, ornidazole and other changes in drug content. Results: garlic injection drug compatibility process, no color change was found Ng liquid precipitation or produce
gas, that is, no chemical or physical reactions. Garlic injection with ceftriaxone sodium, ribavirin, azithromycin, gatifloxacin mesylate compatibility, in the 2h to stabilize; with ornidazole, vitamin C, and amikacin sulfate compatibility, the content is not stability; compatibility with other antibiotics within 2 hours when the pH change is very small, in the allowable range; with ceftriaxone sodium, ribavirin, when compatibility amikacin sulfate, pH value is stable. Conclusion: Garlic injection with ceftriaxone sodium,
ribavirin, azithromycin, gatifloxacin mesylate in 2h in combination; and amikacin sulfate, ornidazole and vitamin C should not be compatibility.
Keywords: Allicin; compatibility; stability; UV spectrophotometry
Allicin Garlic, also known as the new prime, chemical, known as diallyl trisulfide, 1987, Chinese scholars isolated
from garlic bulbs of a sulfur-containing compounds. Garlic
volatile substances, large garlic juice, garlic, allicin leachate, as well as on a variety of pathogenic bacteria in
vitro, such as staphylococcus, meningitis, pneumonia, meningitis, Streptococcus, and diphtheria, dysentery, E. coli, typhoid, tuberculosis and cholera arc bacteria are inhibited or kill the role of clinical trials in the treatment of
cryptococcal meningitis has been, lung and digestive tract fungal infection, white beads in children caused by indigestion and so on. Amikacin sulfate is an aminoglycoside antibiotic, for the majority of bacteria are a good role, commonly used in intravenous drip. As the clinical
complications and speed up the treatment of certain diseases, it is hoped that garlic injection compatibility with other drugs to use, in order to understand the compatibility information and to provide a basis for clinical, we will
garlic injection with other drugs, compatibility studies, reports are as follows.
An instrument and Drug
11 Instruments UV1700 UV-visible spectrophotometer
(Shimadzu, Japan); PHS3C Precision PH (Shanghai Albert Instrument Factory).
12 Injection drug Garlic (Shanghai Hefong Pharmaceutical Co., Ltd., specifications 30mg, batch number 4E38002); ceftriaxone (Shanghai New Asia Pharmaceutical Co., Ltd., specifications 1.0g, batch number 060,111); ornidazole (Wuhan Biochemistry pharmaceutical, specifications 0.25g, batch
number 20,031,001); injection of azithromycin (Hangzhou Macao and Asia Bio-Technology Co., Ltd., specifications 0.25g, batch number 050,117); ribavirin injection (Zhengzhou Cheuk-fung on
pharmaceutical factory , specifications 100mg, lot number
0601204); amikacin sulfate injection (Qilu Pharmaceutical Co., Ltd., specifications 0.2g, batch number 601016FA); Gatifloxacin mesylate, sodium chloride injection stars (Nanjing Chia Tai Ching Pharmaceutical Co., Ltd., specifications 0.2g, lot number 0602282); vitamin C injection (drug days, Hubei Pharmaceutical Co., Ltd., specifications 0.5g, batch number 20,040,822); 5% glucose injection (Shanghai Pharmaceutical Group of the source Changfu Huangchuan Co., Ltd., size 250ml, batch number 06,021,801).
2 Methods
21 reserve liquid preparation of precise preparation of 600μg/ml allicin 5% glucose solution, 500μ/ml azithromycin
5% glucose solution, 2mg/ml gatifloxacin mesylate 5% glucose solution, 50μg/ml ornidazole zole 5% glucose solution, 5mg/ml vitamin C 5% glucose solution.
22 Determination of wavelength selection [1] to 5% glucose solution as a blank on these drugs, glucose solution in the 200 ~ 400nm wavelength range scanned. Allicin can see from the scan pattern Department has maximum absorption at
198.5nm, azithromycin Department has maximum absorption at 265nm, gatifloxacin mesylate Department has maximum absorption at 292nm, ornidazole Department has maximum absorption at 319nm, vitamin C at the maximum absorption at 265.2nm. Comparative map we can see that Allicin from the above four kinds of drugs at the maximum absorption of office there was no corresponding absorption determination of no interference between them.
23 to take the above examination of the linear relationship between reserve liquid, each diluted with 5%
glucose solution obtained linear relationship shown in Table 1.
24 recovery tests check store fluid under 2.2, respectively, by 2.3 distilled water plus an accurate method of preparation of allicin with the drug concentration in a
series of mixed solution were measured in the selected absorption wavelength, and substituted into the regression equation to calculate the recovery rate, the results of Table 2.
Table 1 drugs such as allicin standard curve and linear
range (abbreviated)
Table 2 allicin when the recovery rate of drug compatibility (omitted)
Preparation of liquid 3 compatibility
Simulated clinical drug concentrations, taking injection Allicin 30mg, azithromycin 0.25g, gatifloxacin mesylate 0.2g,
ornidazole 0.25g, Vitamin C0.5g of 5% glucose dissolved and diluted, preparation of Allicin concentration of 60μg/ml,
azithromycin 5mg/ml, gatifloxacin mesylate 2mg/ml, ornidazole 50μg/ml, vitamin C 50μg/ml mixed solution at room
temperature to observe the compatibility changes, at 0h, 2h, 4h, 6h observe the solution appearance changes, and measured pH value and content.
31 appearance of compatibility when adding a large garlic with azithromycin, gatifloxacin mesylate and vitamin C are
colorless and transparent and to clarify the solution, and in no significant changes within 6h and no precipitation generation; ornidazole injection dissolved light yellow solution of allicin and its compatibility to clarify the post-
yellowish transparent liquid, and in no significant changes within 6h and no precipitation produced. Reposted elsewhere in the paper for free download http://
32 pH is not compatibility, when allicin in 5% glucose dissolved in pH = 4.10, Azithromycin 5% glucose dissolved in
pH = 6.94, gatifloxacin mesylate in 5% glucose dissolved in pH = 7.15, vitamin C5% glucose dissolved in pH = 6.81, ornidazole in 5% glucose dissolved in pH = 3.34, Allicin compatibility with the drug after the change in the results shown in Table 3.
Table 3 drug-compatibility Allicin slightly after the pH change
Table 4 Allicin content changes after the drug compatibility slightly
Table 5 of allicin and other drugs, K-ratio method, such
as wavelength pairs of absorption standard curve (omitted)
Table 6 of allicin and other drugs, the recovery rate slightly
33 content changes will be compatibility of fluid in the selected wavelengths were measured, determination of the results shown in Table 4.
4 Results
41 reserve liquid preparation of precise preparation of 600μg/ml allicin 5% glucose solution, 200μg/ml Ceftriaxone
5% glucose solution, 20μg/ml ribavirin 5% glucose solution,
2000μg/ml amikacin sulfate 5% glucose solution.
42 Determination of the wavelength selection [2] Because of garlic injection with the above the maximum UV absorption of several drugs that are mutually superimposed parts, allicin nor suitable for UV-mapping on the isosbestic point, it uses K-ratio determination of other drugs concentration.
Respectively, take appropriate amount of the above-mentioned
solution of 5% glucose solution as a blank were 200 ~ 400nm wavelength range scan, we can see from the scan map map of allicin were no suitable isosbestic point, it measured K-ratio
method using the concentration of other drugs, tested to determine 198.5nm, 247nm wavelength has a better K value (K = 5.97), and ceftriaxone in the 227nm, 248.5nm wavelength Department has such absorption; ribavirin at the 215nm, 199nm wavelength Department has so absorbed ; amikacin sulfate in the 198nm, 195nm wavelength Department has so absorbed.
43 linear relations between the study [3] obtained under the 3.1 store solution, each diluted with 5% glucose solution obtained linear relationship shown in Table 5.
44 recovery tests check store fluid under 3.1, respectively, by 3.3 distilled water plus an accurate method of preparation of allicin with the drug concentration in a series of mixed solution were measured in the selected
absorption wavelength, and substituted into the regression equation to calculate the recovery rate, the results of Table 6.
45 Preparation of simulated clinical fluid compatibility drug concentration, injection taking Allicin 30mg, ceftriaxone 1.0g, ribavirin 100mg, amikacin sulfate, 0.2g of 5% glucose dissolved and diluted into the concentration of the concentration of the preparation of Allicin 60μg/ml,
Ceftriaxone Sodium 10mg/ml, ribavirin 20μg/ml, amikacin
sulfate, 200μg/ml mixed solution at room temperature.
46 observation of the 3.5 compatibility changes in fluid under the compatibility, at 0h, 2h, 4h, 6h observe the appearance of changes in solution, measured pH, determination of absorption.
461 appearance of Allicin and ribavirin, amikacin sulfate
accession, both colorless and transparent to clarify the solution, and in no significant changes within 6h and no precipitation generation; Ceftriaxone powder for injection as a pale yellow solution after dissolution, garlic Su after their compatibility light yellow clear and transparent liquid, and in no significant changes within 6h and no precipitation produced.
462 pH value is not when the compatibility of allicin in 5% glucose dissolved in pH = 4.10, ceftriaxone sodium in 5%
glucose dissolved in pH = 6.07, Ribavirin, in 5% glucose dissolved in pH = 7.77, amikacin sulfate, 5% glucose dissolved in pH = 7.77, large garlic compatibility with the drug after the change in the results shown in Table 7.
Table 7 Allicin compatibility with other drugs after the pH change slightly
463 content changes will be compatibility fluid in the selected wavelengths were measured, the results show that in 0 ~ 6h fluctuating within a small, determination of the results
in Table 8.
Table 8 Allicin compatibility with other drugs, after the content changes slightly
5 Discussion
In this study, garlic injection of several drugs commonly used with clinical compatibility tests carried out research,
provide a basis for the clinical application compatibility.
From Table 4, can be found, garlic injection with azithromycin, gatifloxacin mesylate compatibility, in the 2h is stable within; with ornidazole and vitamin C compatibility, the content of instability, to be furtherResearch. In Table 7 garlic injection with ceftriaxone sodium, ribavirin, compatibility of the process of amikacin sulfate, pH, are basically stable, 2h virtually unchanged. Table 8, the garlic injection with ceftriaxone, Ribavirin, compatibility, content
is stable within 2h; compatibility with amikacin sulfate, the content changes too large, pending further study.
References
A Sotheby Qin, Zhou Li, high-towering garlic and three
kinds of Injection Injection compatible stability of Qiqihar
Medical College, 2003,24 (12): 1337
2 YANG Ji Zhang, Yang Shu-min, such as injection of
gatifloxacin and ceftriaxone Compatible Stability Chinese Journal of Hospital Pharmacy, 2005,25 (3): 256 ~ 257
3 YANG Ji Zhang, Yang Shu-min, such as gatifloxacin for
injection and injection of cephradine compatibility study the stability of Chinese pharmacies, 2005,13,1015 ~ 1017 reposted elsewhere in the paper for free download http://