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Ge bat phenolic alkaloids on experimental cerebral ischemia and hemodynamics_796

By Eugene Stewart,2014-10-30 18:27
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Ge bat phenolic alkaloids on experimental cerebral ischemia and hemodynamics_796

    Ge bat phenolic alkaloids on experimental cerebral ischemia and hemodynamics

     Abstract Objective To observe the bats Ge phenolic

    alkaloids (PAMD) on rat hemodynamics, and further explore the protective mechanism of cerebral ischemia. Methods The method

    of the external carotid artery ligation was observed PAMD rat cerebral blood flow, cerebral vascular resistance, blood pressure, heart rate and other effects. Results PAMD can increase cerebral blood flow, lower cerebral vascular resistance, had no significant effect on heart function. Conclusion PAMD protective effect against cerebral ischemia with increased cerebral blood volume.

     Key words bats, Ge phenolic alkaloids cerebral ischemia cerebral blood flow cerebral vascular resistance

     Effect of Phenolic Alkaloids from Menispermun dauricum on Hemodynamics in Experimental Cerebral Ischemia

     Abstract: ObjectiveTo investigate the effect of phenolic alkaloids from Menispermun dauricum (PAMD) on hemodynamics in rat and explore the mechanism of action for cerebral ischemia.MethodsThe external carotid artery of rat was ligated to observe the effect of PAMD on cerebral blood flow (CBF) , cerebral vascular resistance (CVR), blood pressure (BP) and heart rate (HR). ResultsPAMD could increase CBF and decrease CVR, but had no effect of cardiac function.ConclusionPAMD pocesses protective action on cerebral ischemia and the mechanism of action is related to the increase of CBF and the

decrease of CVR.Increase of CBF may be one of the protection

    mechanism

     Key words: Phenolic alkaloids from Menispermun dauricum; Cerebral ischemia cerebral blood flow; Cerebral vascular resistance

     Menispermum phenolic alkaloids (PAMD) Menispermi from traditional Chinese medicine extracted from a mixture of a

    variety of fat-soluble alkaloids, the main ingredient dauricine and daurisoline. The research group in recent years, studies have shown that, PAMD has good effect of anti-cerebral

    blood shortage, can reduce the water content of brain tissue,

    inhibit malondialdehyde (MDA) and enhanced production of superoxide dismutase (SOD) activity. The experimental observation PAMD on experimental cerebral ischemia in rat cerebral blood flow, cerebral vascular resistance, blood pressure, heart rate and other effects to further explore the depth of anti-cerebral ischemia in rats.

     1 Equipment

     1.1 Animals

     Wistar rats, male, body weight (250 ? 20) g,

    Heilongjiang University of Chinese Medicine from the Experimental Animal Center.

     1.2 Drugs and reagents

     PAMD made by the Task Force, with the first time in order to 1mol / L hydrochloric acid dissolved, and then 1 mol / L sodium hydroxide adjusted to neutral pH, add distilled water to the desired concentration. Nimodipine tablets, Tianjin

    Central Pharmaceutical Co., Ltd., batch number: 020,106.

     1.3 Instrument

     BIOPAC 16 Nihon Kohden.

     2 Methods

     2.1 Sub-section and delivery Methods Wistar rats were 50, were randomly divided into five groups, namely, PAMD high,

    medium and low-dose group, nimodipine group and blank control group, 10 in each group. Of each group according gavage dose of 6 d, the 7th day implementation of the operation.

     2.2 Model of intraperitoneal injection of pentobarbital sodium (30 mg / kg) anesthesia. Back-bit fixed on the

    operating table, right upper abdominal incision, exposing the duodenum, to prepare for duodenal administration. Separation of the left common carotid artery and carotid artery, and the external carotid artery ligation, use 1mm electromagnetic flow probe placed in the common carotid artery (the diameter consistent with the common carotid artery diameter), with the common carotid artery blood flow reflects the supply of the brain internal carotid artery blood flow [1,2]. Separation of

    the right common carotid artery, baroreceptor record of blood pressure after intubation (mmHg). Inserted electrodes to monitor limb lead ECG criteria ?.

     2.3 recorder Observation complete the preparatory work, such as animals, stable condition, after the drugs were

    simultaneously recorded before and immediately after administration 5,10,30,60,90,120 min at different time points of the common carotid artery blood flow, electrocardiogram, heart rate, blood pressure and so on. Cerebral vascular resistance at both ends of the pressure difference with blood vessels and blood flow ratio, said, namely: cerebral vascular resistance = pressure / cerebral blood flow, in units of mmHg * min-1 * ml-1.

     2.4 Data processing of all data to ? s said that among

    the groups using t test. Reposted elsewhere in the paper for free download http://

     3 Results

     3.1 pairs of cerebral blood flow influence the outcome in Table 1. Table 1 shows that by the middle dose group PAMD drugs before the drugs and medicine immediately after the 5

    min cerebral blood flow was significantly increased, statistical results showed that the difference compared with the control group significantly (P

     3.2 pairs of cerebral vascular resistance in the results in Table 2. Table 2 shows PAMD drugs in pre-dose group,

    immediately after drug, medicine 5,60,90,120 min after the cerebral vascular resistance decreased significantly, statistical results showed that the difference compared with the control group significantly (P

     3.3 The impact of blood pressure results in Table 3. Can be seen from Table 3 nimodipine group 5,10,30,60,90,120 min post-drug blood pressure decreased significantly, statistical results showed that the difference compared with the control group significantly (P

     3.4 pairs of heart rate influence the outcome in Table 4. Can be seen from Table 4 nimodipine drug significantly slow down the heart rate after 90,120 min, statistical results showed that the difference compared with the control group significantly (P

     4 Discussion

     The main ingredients of PAMD dauricine (dauricine, Dau), and daurisoline (daurisoline, DS). In view of the lower monomer content Dau and the DS, while the PAMD is relatively high, and the extraction process is relatively simple, so

    Ruoqiang PAMD for clinical use as an effective site that will have important practical significance.

     The brain is an organ most sensitive to hypoxia, its activities mainly depend on aerobic oxidation of glucose to provide energy, once the longer ischemic time can cause

    serious irreversible damage [3,4]. Brain tissue is almost no energy reserves, need a continuous supply of blood circulation, oxygen and glucose. When the acute occlusion of intracranial arteries, the brain's blood circulation was blocked, the brain regional blood flow, reduce, and often accompanied by focal vascular paralysis [5]. Therefore, early treatment after cerebral infarction and restore blood supply and prevent the occurrence of ischemia-reperfusion injury of

    ischemic stroke treatment and prognosis is very positive.

     This study found that PAMD can increase cerebral blood flow and reduce vascular resistance, and blood pressure and heart rate had no significant effect, suggesting that PAMD mechanism against cerebral ischemia may be related to a

    remarkable expansion of the brain blood vessels, and weak

    peripheral vasodilation related to the right cerebral infarction patients with recanalization of the establishment of collateral circulation, semi-dark with a very favorable

    recovery of neurological function.

     References

     [1] TSUI Shuk Wan. Pharmacology experiments methodology [M]. Beijing: People's Health Press, 1998:954.

     [2] Chen Qi. TCM Pharmacology Research Methodology [M]. Beijing: People's Health Press, 2005:396.

     [3] Lu YM.Daurisoline, as a novel N-type calcium channel

    blocker [J]. Acta Pharmacol Sinica, 1992,13:83.

     [4] Dawson V, Brahmbhatt HP, et al.Expression of inducible nitric oxide synthase delayed neurotoxicity in primary mixed neuronalglial cortical cultures [J].

    Neuropharmacology, 1994,33:1425. Reposted elsewhere in the paper for free download http://www . hi138.com

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