DOC

Ginkgo biloba and ginkgo biloba extract and its tablet in thin-layer chromatography analysis of terpene lactones_608

By Josephine Powell,2014-10-30 18:37
14 views 0
Ginkgo biloba and ginkgo biloba extract and its tablet in thin-layer chromatography analysis of terpene lactones_608

    Ginkgo biloba and ginkgo biloba extract and its tablet in thin-layer chromatography analysis of terpene lactones

     Abstract Objective To study the Ginkgo biloba and ginkgo biloba extract and terpene lactones in Ginkgo leaf TLC. Methods of Ginkgo biloba extract, bilobalide, ginkgolide A, ginkgolide B and ginkgolide C as a target to study the effects of temperature on the thin-layer chromatography. The results

    of the temperature had no significant effect. Conclusion The TLC method can be carried out at room temperature.

     Key Words Ginkgo biloba extract; terpene lactones; thin-

    layer chromatography; temperature

     Analysis of Terpene Lactones from Ginkgo biloba Leaf and Ginkgo biloba Extract and Ginkgo Leaf Tablets by TLC

     Abstract: ObjectiveTo study the method of identification of terpene lactones from Ginkgo biloba leaf and Ginkgo biloba extract and Ginkgo Leaf tablets by TLC.MethodsWith Ginkgo biloba extract, Bilobalide, Ginkgolide A, Ginkgolide B, Ginkgolide C as objects, study the interference of the

    temperature to TLC.ResultsThe temperature showed no interference.ConclusionThis method of identification of TLC can carry on under the indoor temperature.

     Key words: Ginkgo biloba extract; Terpene lactones; TLC; Temperature

     Ginkgo biloba [1] for the Ginkgo Ginkgo biloba L. Ginkgo biloba plants of dry leaves, with a convergence lungs, asthma, promoting blood circulation Huayu, analgesic effect. Ginkgo

biloba extract [1] is made of Ginkgo biloba extract processed

    with promoting blood circulation Huayu Tongluo function; Ginkgo leaf extract is made by processing the film, with the effectiveness of Ginkgo biloba extract. Terpene lactones of its active components, mainly including bilobalide, ginkgolide A, ginkgolide B and ginkgolide C and so on. For the ginkgo leaf, ginkgo biloba extract and Ginkgo biloba, "Chinese Pharmacopoeia" in 2005 used by the Department ? were

    identified by TLC in its terpene esters composition of thin-

    layer plate with 4% acetic acid sodium carboxymethyl fiber

    sodium solution as adhesive silica gel G thin-layer plate,

    reference substance solution as the reference substance bilobalide, ginkgolides A reference substance, reference substance ginkgolide B, ginkgolide C reference substance mixed

    solution or ginkgo biloba the control extract solution; launch agent for the toluene - ethyl acetate - acetone - methanol

    (10:5:5:0.6), expand the temperature below 15 ?. In this

    study, a "Chinese Pharmacopoeia" method, select at different temperatures under the control of Ginkgo biloba extract, bilobalide reference substance, ginkgolides A reference substance, reference substance ginkgolide B, ginkgolide C reference substance of the chromatographic behavior has been studied experimentally.

     An instrument and reagent

     Refrigerator; Expand cylinder, ultra-violet light;

    control of Ginkgo biloba extract (China Pharmaceutical and Biological Products Institution, lot number 110866 200202);

    bilobalide reference substance (China Pharmaceutical and

    Biological Products Institution, lot number 110865 200404);

    ginkgolide A reference substance (China Pharmaceutical and Biological Products Institution, Lot 0862 2004); ginkgolide

    B reference substance (China Pharmaceutical and Biological Products Institution, lot number 110863 200305); ginkgolide

    C Reference Substances (China Drugs and Biological Products Institution, batch number 110864 200304); other reagents

    were analytical pure.

     2 Methods and Results

     2.1 Expand the investigation started when the temperature

usually from 20 ? ambient temperature to 4 ? change does not

    affect the start time and the Rf values, volatile compounds and terpene hydrocarbons for low-temperature thin-layer

    separation is required to start when the temperature sometimes

    changes in the role of a variety of other separation parameters, especially the degree of saturation will be started indoors due to temperature changes and the change in [2].

     2.2 of Ginkgo biloba extract solution prepared in control

    precision, said taking control of Ginkgo biloba extract of 0.15 g, water 10 ml, set in hot water bath to dissolve powder, add two drops of 2% hydrochloric acid solution, extracted with ethyl acetate Zhenyao 4 times (15, 10,10,10 ml), combined extracts with 5% acetic acid 20 ml washing, sub-taking sodium

    acetate solution, and then 10 ml ethyl acetate, washing the combined ethyl acetate solution, recovery of solvents from dry residue was dissolved in methanol and transferred to 5 ml Liang Ping, add methanol to the scale, shake, filtration, take added filtrate that was.

     2.3 Preparation of reference substance solution obtained, respectively, said precision reference substance bilobalide, ginkgolides A reference substance, reference substance ginkgolide B, ginkgolide C amount of reference substance, precision, said set, plus methanol produced per milliliter of the 1,1,1,0.5 mg-containing solution, as a reference substance solution. Reposted elsewhere in the paper for free download http://

     2.4 Preparation of reference substance mixed solution were collected from reference substance bilobalide, ginkgolides A reference substance, reference substance ginkgolide B, ginkgolide C amount of reference substance, precision, said set, plus each containing methanol produced

    per milliliter 1,1,1,0.5 mg of the mixed solution, as a reference substance mixture.

     2.5 Determination of drawing the control of Ginkgo biloba extract solution, the reference substance solution and reference substance mixtures of 15 μl, respectively, at the

    same point with 4% acetic acid sodium carboxymethyl cellulose sodium solution as adhesive silica gel G thin-layer board,

    with toluene - ethyl acetate - acetone - methanol (10:5:5:0.6)

as the agent, respectively, 10,12,14,16,19,21,24,27,30,33 ,

    35 ? temperature started out, dried, smoked with acetic acid vapor 15 min, at 140 ~ 160 ? heating 30 min, let cool, set UV lamp (365 nm) under review. The results shown in Table 1 and Figure 1.

     Table 1 The experimental temperature of the spots

    corresponding to the number of different solutions and the relative position (abbreviated)

     2.6 Results in control of Ginkgo biloba extract, bilobalide reference substance, ginkgolides A reference substance, reference substance ginkgolide B, ginkgolide C

    reference substance in the above-mentioned temperature

    stability, and at different temperatures of TLC behavior of non - significant changes.

     Figure 1 Chromatogram of Ginkgo terpene lactones (omitted)

     3 Conclusion

     In this study, a "Chinese Pharmacopoeia" method, select at different temperatures under the control objects of Ginkgo biloba extract, bilobalide reference substance, ginkgolides A reference substance, reference substance ginkgolide B, ginkgolide C reference substance for color spectral analysis, the results in the above selected temperature range, the composition and stability, spots were clear, round the whole, the relative position of the corresponding spot was no significant difference, namely, choose temperature

    chromatogram with the "Chinese Pharmacopoeia", under the provisions of temperature The chromatogram in line, the temperature had no significant effect, so ginkgo leaves, ginkgo leaves the control extract, Ginkgo leaf in terpene lactones (bilobalide, ginkgolide A, ginkgolide B, ginkgolide C), thin Layer Chromatography can be carried out at room temperature.

     References

     [1] State Pharmacopoeia Commission. Chinese

Pharmacopoeia, ? Department of [S]. Beijing: Chemical

    Industry Press, 2005:220,281,597.

     [2] Li Wei, XIAO Xiang Lin, Dan-Yan. Commonly used thin-

    layer chromatography identification of Traditional Chinese Medicine [M]. Beijing: Chemical Industry Press, 2004:38. Reposted elsewhere in the paper for free download http://

Report this document

For any questions or suggestions please email
cust-service@docsford.com