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Ginger oil kill Experimental Study of Schistosoma japonicum cercariae_124

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Ginger oil kill Experimental Study of Schistosoma japonicum cercariae_124

    Ginger oil kill Experimental Study of Schistosoma japonicum cercariae

     Author: Bian Li Huang Boqing Ding Ling Xia Peng Zang

     Abstract Objective To investigate the ginger oil to kill the effect of Schistosoma japonicum cercariae. Methods Ginger

    Oil 100μl on the glass slide to observe the ginger oil to kill schistosome cercariae time; and use has been to kill the mice infected with Schistosoma japonicum cercariae, after perfusion plus parasite was collected pieces, calculate

    infection rates. The results of ginger oil in the 20s can quickly kill within Schistosoma japonicum cercariae; have to kill the mice infected with Schistosoma japonicum cercariae were not seized Schistosoma adult infection rate of 0; while the control group, 93% infection rate; difference in infection rates between the two comparison statistically significant (P = 0.000). Conclusion Ginger oil has a better kill schistosome cercariae results.

     Key words ginger oil; Schistosoma japonicum cercaria

     The Experimental Study of the Ginger Oil on Killing Schistosoma japonicum Cercariae

     Abstract: ObjectiveTo evaluate the efficacy of the Ginger oil killing the Schistosoma japonicum Cercariae. Methodsthe Ginger oil were loaded on the slides, and the time of killing

    the Schistosoma Cercariae was recorded. The treated Schistosoma Cercariae were subsequently used to infect mice by the method of pouring and tearing them into shreds. At last

the infected efficiency was counted.ResultsThe Ginger oil

    could effectively kill the Schistosoma Cercariae in 20 seconds. The killed Schistosoma Cercariae were noninfectious to mice, but the infected efficiency of the control groups was 93% significant difference between the two treatments (P = 0.000). ConclusionThe Ginger oil can offer a satisfactory killing efficiency against the Schistosoma japonicum Cercariae.

     Key words: Ginger oil; Schistosoma japonicum cercariae

     Zingiberaceae Ginger Ginger is a genus of the ginger rhizome Zingiber officinale Rosc, ginger oil is extracted from the ginger rhizome of volatile oil is rich in aromatic smell, is mainly used for food and beverages plus fragrant seasonings are also medicinal raw materials [1 , 2]. To understand the ginger oil to kill the effect of Schistosoma japonicum

    cercariae to carry out the experiments. Now report on the results as follows.

     1 Materials

     1.1 Ginger Oil from Wuhu Shanshan Biotechnology Ltd., using supercritical carbon dioxide extraction, in order to red-brown oily liquid. Ginger oil relative density (20/200C) 0.925, refraction coefficient (200C) 1.499.

     1.2 10% chloral hydrate Tianjin Chemical Reagent Factory Chupin Fu-chen.

     1.3 cercariae by the Jiangsu Provincial Institute of schistosomiasis prevention and control infected snail,

    experimental day-Yi Fang Schistosoma japonicum cercaria.

     1.4 Mice Kunming mice, 3-month-old rats re-20 ~ 24g, male

    and female in half, by the Institute of Experimental Animal Center.

     2 Methods

     2.1 Experiment kill cercariae Schistosoma cercaria

    release date Plaza. Ginger oil slide 100μl take place, each

    piece by adding about 100 live cercariae done 5 / time, to do a total of two times, killing cercariae dissection microscope

observation of recorded time. In order to reach cercariae

    needle anatomy, not activists sentenced to death, to death for killing all cercariae cercariae time. Dechlorination of water to establish a separate control group.

     2.2 Animal experiments in mice infected with shaving the abdomen (range, 2 cm × 3 cm), subcutaneous injection of 10%

    chloral hydrate 0.1 ml to anesthesia. Take Ginger Oil 100 μl

    in the glass slide, add Schistosoma cercaria (40 ? 5) section

    in which to place 30 min after anesthesia in mice have been affixed to the abdomen, infection 20 min, infected 10 mice. 40 d after the autopsy, with added tear perfusion method to collect parasites. First, perfusion through the superior vena cava washing, anatomy microscope, counting the liver, intestinal parasites within the number of venous plexus;

    lavage end, remove the liver, intestine, using tweezers to pull pieces of liver and mesenteric, inspect, count is not out of The parasites, calculate infection rates. Dechlorination of water infected with a separate control group. Reposted elsewhere in the paper for free download http://

     3 Results

     3.1 Experimental ginger oil to kill cercariae within an average of 20 s can quickly kill the cercariae of Schistosoma japonicum; and dechlorination of water in the control group Schistosoma cercariae until the glass slide with water dried up (1 ~ 2h) the overwhelming majority movement and lively. Ginger Oil 2 times the time to kill schistosome cercariae in Table 1. Table 1 ginger oil to kill Schistosoma japonicum cercaria time (omitted)

     3.2 was to kill the mice infected with Schistosoma japonicum cercariae results by ginger oil to kill the mice infected with Schistosoma japonicum cercariae were not seized upon Schistosoma adult infection rate of 0; by the dechlorination of water infected with the control group of 14 mice survived except one adult worm is not only the seizure, the other 13 were seized in Schistosoma adult infection rate of 93%. Under the SPSS software, using fisher's exact probability method was P = 0.000. Ginger liquid group compared

    with control group prevalence of Schistosoma japonicum cercariae significant difference statistically significant. Table 2. Table 2 is to kill the mice infected with Schistosoma

japonicum cercariae experimental results (omitted)

     4 Discussion

     Ginger Ginger oil mainly composed of alcohol, ginger-ene,

    ginger ketene, etc., and its role as antioxidant, antibacterial, anti-coagulation and anti-thrombosis,

    regulating blood lipids and prevention of atherosclerosis as well as the stomach and vomiting, nourish the liver and

    gallbladder , anti-inflammatory, analgesic, blood circulation Quhan, avoid the stench; treatment of rheumatoid arthritis and other diseases [3 ~ 7]; but not yet see the ginger oil to kill schistosome cercariae reported. The observation results show

    that ginger oil has a better kill Schistosoma japonicum cercariae role in the 20s can quickly kill within Schistosoma japonicum cercariae; have been to kill experimental animals infected with cercariae of Schistosoma have not seized

    Schistosoma adult infection rate of 0 ; while the control group prevalence of 93%. Comparison between the two P = 0.000, significant difference statistically significant. Ginger oil that can really kill schistosome cercariae have been killing cercariae of Schistosoma infection in experimental animals, no. Ginger oil on the role of schistosome cercariae of the killing may be related to volatile oil of ginger alcohol,

    ginger-ene, ginger and other chemical constituents on ketene and its detailed mechanism remains to be further explored. The experimental results for the next graduate ginger oil to prevent infected with Schistosoma japonicum cercariae provide an experimental basis.

     References

     [1] Yi-Qiang Ge, Ni Yuan-Ying, Zhang Zhenhua, et al.

    Ginger Essential Oil Research Progress [J]. Chinese spices, 2004,9:9.

     [2] Wen-shan, Yan Yuxia. Ginger chemical constituents and biological activity profile [J]. Pharmaceutical journal,

    1999,20 (5): 217.

     [3] Wang Ying, Dong-Wei Li. Ginger research [J]. Chinese medicine, 2006,15 (9): 63.

     [4] Lu Chuan-jian, OU Ming, Wang Ning. Analysis of the chemical constituents of ginger overview [J]. TCM medicine and clinical pharmacology, 2003,14 (3): 215.

     [5] CUI Qing-xin, Dong Yan. Ginger essential oil chemical constituents of GC-MS analysis [J]. Liaocheng University

    (Natural Science Edition), 2006,19 (2): 43.

     [6] Xu Li, Chen-ze will, Yuan Yaqin. Ginger oil on the

    antioxidant effect of lard [J]. Grain and Oil processing and food machinery, 2004, 10:45.

     [7] weeks Mengqing. Antimicrobial activity of ginger extract and its application [J]. Feed the world, 2006,1:27. Reposted elsewhere in the paper for free download http://

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