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Fresh olive leaf volatile oil composition by gas chromatography - mass spectrometry_903

By George Boyd,2014-10-30 18:05
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Fresh olive leaf volatile oil composition by gas chromatography - mass spectrometry_903

Fresh olive leaf volatile oil composition by gas

    chromatography - mass spectrometry

     Abstract Objective To analyze the volatile oil

    constituents of fresh olive leaf. Methods steam distillation of fresh olive leaf volatile oil by gas chromatography - mass

    spectrometry (GC-MS) were analyzed, normalized measured by the relative content of each component. Results Identification of fresh olive leaf volatile oil compounds in the 47 components, accounting for the relative total content of 99.7%. Conclusion

    of fresh olive leaf volatile oil chemical composition and their relative content of trans -3 - hexene -1 - ol (36.58%),

    iso-aromadendrene (16.26%), β-caryophyllene (6.51%), hexanal

    (5.66 %), alien alcohol (4.92%), 2 - ethoxy-propane (4.78%),

    cis--2 - E en -1 - ol (3.10%), cis -3 - hexene acetate

    (2.89% ), methyl salicylate (2.32%), β-Cubebene (1.79%), Gan

    Xiang-ene (1.35%), and so on.

     Key Words fresh olive leaf volatile oil and gas

    chromatography - mass spectrometry

     AbstractObjectiveTo analyze the constituents of the essential oil from fresh Canarium album Raeusch leaves.Methods Essential oil from the fresh Canarium album Raeusch leaves was extracted by steam distillation and were analyzed by gas chromatography-mass spectrometry (GC / MS). Results Forty-six

    compounds were identified, which accounted for over 99.7% of the essential oil. Conclusion The main components of essential oil include trans-3-hexenol (36.58%), Alloaromadendren

    (16.26%),. beta.-caryophyllene (6.51%), hexanal ( 5.66%),

    isohexanol (4.92%) ,2-ethoxypropane (4.78%), cis-2-peneten-1-

    ol (3.10%), cis-3-hexenyl acetate (2.89%), methyl salicylate (2.32%) and etc.

     Key wordsFresh Canariumalbum Raeusch leaves; Essential oil; GC-MS; Component analysis

     Olive Branch plant olive Canariumalbum (Lour.) Raeusch., For the tall trees, commonly known as the Green Lam, white rugby and so on, originated in China and in Fujian, Guangdong, Guangxi, Taiwan, Yunnan, Sichuan and other provinces for many in our country has 3000 years of cultivation history; stone circle moment for the Egg, was green when ripe, so called "fruit." According to "Compendium of Materia Medica" contains: "Olive to Health fluid, only polydipsia, cure sore throat, chemical product fresh. Chew swallow juice can answer all

    Yubie poison." Olive (fruit) can rule carnivorous fish bone stuck throat and poisoning can also hangover drugs to reduce cholesterol. Yan-Ling Liang et al [1] reported that olive plants of Ukraine Lam Canarium pimela Koening leaf water

    extract with a step-down and slow down the heart rate

    Pharmacodynamics. Green Lam fruit volatile oil components have been reported in the literature [2,3], green leaf volatile components Lam reported in the literature yet to see. In this study, extracted by steam distillation of fresh olive leaf volatile oil, gas chromatography - mass spectrometry analysis

    of fresh olive leaf volatile components were analyzed.

     1 Materials and methods

     1.1 sample sources and the extraction of volatile components in samples collected from Guangdong Longmen Nankunshan feet, age about 180 years of fresh fruit olive leaves. The fresh olive leaf crush, saying that taking 200 g, using steam distillation 3 h, distillate extracted with anhydrous ether, extract dried with anhydrous sodium sulfate,

    35 ? water bath, vacuum recovery ether, had orange ribbons there are micro-Xin Lam smell fragrant volatile oil 0.052 g, have the oil rate of about 0.026%. Analysis of volatile oil from pre-airtight container stored at 10 ? below. Anhydrous

    ether and anhydrous sodium sulfate were of analytical grade reagents.

     1. 2 GC / MS instrumentation and analytical conditions the United States Finnigan Voyager GC - MS instrument. Column

    DB-5MS (30 m × 0.25 mm × 0.25μm), carrier gas He, flow rate

    1 ml / min, sample volume 0.02 μl, split injection, split

    ratio 20:1; column initial temperature of 60 ?, maintain 3

min, then 10 ? / min up to 260 ?, and then maintain a 3 min;

    ionization mode EI; interface temperature of 230 ?, ion

    source temperature of 200 ?; ionization energy 70 eV;

    multiplier voltage of 400 V; scan quality range of 35 ~ 450 amu. Standard spectral library for the United States LIBTX and the NIST spectral library. Reposted elsewhere in the paper for free download http://

     2 Results and discussion

     Figure 1 is a fresh olive leaf volatile oil composition of the total ion chromatogram, a total of 49 isolated peaks, using a computer search of all peaks corresponding mass map identified 46 compounds, with peak area normalization method

    was determined by their The relative content. The results in Table 1. Volatile components were identified accounting for 99.7% of the total composition.

     Table 1 data show that the volatile components of fresh olive leaves are mainly alcohols, terpenes, aldehydes, ketones, esters, and most also contain unsaturated bonds, such as: enol type, allyl aldehyde, allyl esters and other substances. Of fresh olive leaf volatile components in the

    methyl salicylate has a fragrant smell, as a traditional folk medicine for pain and convergence, pass through, Qufeng, urination, excitement and so on efficacy for the treatment of headache, abdominal pain, joint pain, inflammation, rheumatism, sore throat, skin diseases, dental conditions such as wind [4]; β-elemene with a broad-spectrum anti-tumor,

    curative effect, drugs, mild side effects, etc., to β-elemene

    as their principal ingredients Eiemene injection is the success of China's independently developed Type II anti-tumor

    drug, is currently widely used in clinical malignant serous effusion, lung cancer, gastrointestinal cancer, brain tumors and other treatment of superficial tumors [5 ]; Caryophyllene is the main component of natural flavors and fragrances. We

    hope that the volatile components of fresh olive leaf's study can in order to better development and utilization of resources to provide a scientific basis olive leaf. Table 1 fresh olive leaf volatile oil chemical constituents and their

    relative content (abbreviated)

     References

     [1] Yan-Ling Liang, DONG Yan-fen, LUO Yan, et al.

    Ukraine Lam leaves water extract on blood pressure and heart rate of the experimental study [J]. Jiangxi Medical Journal, 2006, 46 (3): 42.

     [2] Li-Juan Zhao, Xin Guang, Zhang Jie Li. By gas chromatography - mass spectrometry analysis of Fujian olives, olives meat and olive-jen volatile oil components [J]. Food Science, 2005,26 (9): 391.

     [3] Ming Zhong, Yu-Fen Chen, Gan Lian-sheng, and so on.

    Winter pitch chemical composition of olive fruit volatile oil analysis [J]. Fruit Sinica, 2004,21 (5): 494.

     [4] Mu Zhang, Yang Jing. Yunnan Gaultheria and Utilization of plant essential oils [J]. Pharmaceutical Journal, 1966, 13 (6): 451.

     [5] to spend Wenfeng, CAI Shao-hui. β-elemene anti-tumor

    effect of basic and clinical research [J]. Chinese herbal medicines, 2006, 22 (1): 93. Reposted elsewhere in the paper for free download http://www . hi138.com

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