Extraction of flavonoids in Brassica juncea and their hydroxyl radical scavenging effect of
【Abstract】 Objective To investigate the mustard in the extraction of total flavonoids, identification method and scavenging of hydroxyl radicals in order to make full use of
mustard plant resources and avoid waste of resources. Methods ultrasonic extraction of ethanol extracted from the mustard flavonoid substances, a flavonoid extracted to verify, and concentration was determined by spectrophotometry, using
mustard flavonoids on hydroxyl radical scavenging experiment. The results measured by the total flavonoid content of the sample C = 0.4450 mg / ml, the recovery rate was 100.7%, the purity and yield are high. Conclusion This method uses the
whole physical process without any pollution, the extraction of flavones from mustard in an effective way. Mustard flavonoids extract of OH radicals generated by Fenton system, a very good scavenging effect.
Key words ultrasonic extraction of total flavonoids
identification of radical scavenging mustard
Extraction of Total Flavone from Bragica juncea Coss and Its Effects on Scavenging of Hydroxyl Radicals
Abstract: ObjectiveTo make use of the resources of Bragica juncea coss, avoid wasting and approach the extraction of total flavanone of Bragica juncea Coss and its effects on scavenging of hydroxyl radicals. MethodsThe flavonoids were extracted by ethanol as the solvent from Bragica juncea coss with ultrasonic wave and ethanol extraction. Using
spectrophotometry to extract and check the flavone of Bragica
juncea Coss.Results The effect of extracts of Bragica juncea Coss scavenging of hydroxyl radicals was studied. By this method getting the density of the total flavone of Bragica
juncea Coss was C = 0.4450mg/ml and the rate of recovery was 100.7%. The outcome and the purity of the flavone were all very high. ConclusionThis method is a purely physical process and has not any pollution. It is an ideal way to extract the flavone of Bragica juncea coss. Total flavone from Bragica juncea Coss has effects on scavenging on hydroxyl radicals.
Key words: Ultrasonic wave treatment; Bragica juncea Coss; Total flavone; Identification; Hydroxyl radical; Scavenging
Mustard Bragica juncea Coss, also known as mustard,
potherb mustard, wrinkled leaf mustard, yellow mustard, for cruciferous plants mustard Brassia juncea (L.) Czern.et Coss oil mustard Brassia juncea (L.) Czern.et Coss.Var.gracilis Tsen et Lee's Spear leaves. Are cultivated all over the
country. Wash leaves in spring mining, civil frequently used to do pickles. Mustard sexual Weixin, Wen. Go lung, stomach, kidney. Li Huo Tan with gas, temperature in the spleen, dispelling wind scattered the blood, reduces swelling, pain and other effects. Indications Hantan cough, Xiongge unfavorable Wei Han deprivation, diarrhea, flu cold and bone injury, joint pain embolism . There are currently studies have shown that : Mustard contains flavonoid glycosides, acid resin, tannins, etc., including flavonoids and other
active ingredients for treating coronary heart disease, senile dementia, cerebral thrombosis, nervous system diseases and eliminate free radicals, antimicrobial, anti-cancer and so a
significant effect, non-toxic side effects, and thus developed
a variety of medicines and health food products.
Current home and abroad for mustard Flavonoids relative lack of study. Ethanol extraction is currently useful for extracting flavonoids mustard in a report , however, the relatively long time to extract, process more complex, extraction efficiency is low. In recent years, ultrasound technology, the extraction of plant alkaloids, glycosides, biologically active substances, animal tissue study of pulp and other toxic substances have been reported [4,5],
indicating that its has a low power consumption, high efficiency, non - destruction of the characteristics of the
active ingredient. Many studies have shown that the use of ultrasonic vibrations generated by a strong, high acceleration, strong cavitation, mixing function, etc., can accelerate the active ingredients of plant material into the solvent, thus increasing the extraction rate of the active ingredients, shortening extraction time, and also can avoid the impact of high temperature on the extraction composition , but the ultrasonic technology to extract the mustard in flavonoid research has not been reported. This paper reports the mustard with the ultrasonic extraction of total flavonoids, identify and extract flavonoids on hydroxyl
radical scavenging effect.
An instrument and reagent
1.1 Instrument KQ5200DB NC Ultrasonic Cleaner (Ultrasonic frequency 40 kHz, Kunshan City, Jiangsu Province, Ultrasonic Instrument Co., Ltd.); UV-755B UV-Vis spectrophotometer
(Shanghai Analytical Instruments Factory); ZF-I-type three -
UV analyzer (Shanghai Optical Instrument Factory Gucun); SHB-
III Multi-purpose circulating water pump (Zhengzhou Great Wall Industry and Trade Branch Co., Ltd.).
1.2 Reagents 95% ethanol, AR; sodium nitrite AR; aluminum
nitrate AR; sodium hydroxide AR; three aluminum chloride AR; hydrochloride, AR; ammonia AR; glacial acetic acid AR; Ethyl acetate AR; acid magnesium AR; magnesium powder AR; n-butanol
AR; rutin standard (Chinese medicines and biological
products); hydrogen peroxide AR; ferrous sulfate AR; acid AR; mustard, were collected from Guangxi Baise City.
2 Methods and Results
2.1 Extraction of total flavonoids to take fresh mustard, drying, crushing. Weigh mustard powder 6 g, add 80 ml 95%
ethanol, using ultrasonic extraction 2.5 h, leaching. Residues together with 80 ml 95% ethanol, using ultrasonic extraction 2.5 h, leaching, merging filtrate, vacuum recovery of ethanol to the filtrate until the remaining 25 ml, placed in 250 ml
flask, with 60% ethanol was diluted to scale, may sample fluid.
2.2 Determination of rutin as reference substance based on determination of total flavonoids content of mustard, join
the aluminum ion reagent, while controlling suitable pH value,
and the flavonoid compounds and aluminum salts to form complexes, in the visible region can get stable characteristic absorption peaks .
2.3 quantitative test - Determination of total flavonoids
2.3.1 Wavelength selection to take samples of fluid
amount, in 0.30 ml 5% sodium nitrite solution under alkaline conditions exist, after aluminum nitrate color, with its reagent blank reference solution in the wavelength range 420 ~ 700 nm measured network compounds of the absorbance at 510 nm complex with maximum absorption wavelength, so when you use the wavelength of measurement.
2.3.2 Standard curve drawing lessons were precise control fluid rutin (0.10 mg / ml) 0.00,0.50,1.00,2.00,3.00,4.00,5.00 ml flask at 10.00 ml, respectively of 5% sodium nitrite
solution, 0.30 ml, shaken, put it aside for 6 min; plus 10% aluminum nitrate solution, 0.30 ml, shaken, put it aside for 6 min; plus 4% sodium hydroxide solution 4.00 ml, with 60% ethanol was diluted to scale, shake, put it aside for 12 min,
with reagent blank as reference solution, measured at absorbance at 510 nm. The results in Table 1. Table 1 UV-Vis
spectrophotometer determination of rutin in control solution absorbance results (omitted)
According to Table 1 were the regression equation: A =-
0.0221 13.7578C, r = 0.9999.
2.3.3 Determination of precision extract fluid samples absorb 1.00 ml, owned 10.00 ml flask, according to the standard curve preparation method of determination of absorbance, A = 0.368, according to the regression equation: A
=- 0.022 1 13.757 8 C, r = 0.999 9 sample calculation of total flavonoid content of C = 0.445 0 mg / ml.
2.3.4 Experimental precision the amount of recovery to take samples of liquid 1.00 ml, by adding the standard rutin reference substance 1.00 ml (0.10 mg / ml), with the former samples were measured absorbance measurement operation, find the recovery rate was 100.7%. Reposted elsewhere in the paper for free download http://