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Fallax extracts on mice experimental liver injury in rats_309

By Juan Nelson,2014-10-30 17:42
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Fallax extracts on mice experimental liver injury in rats_309

Fallax extracts on mice experimental liver injury in rats

     Abstract Objective To study the fallax extracts on mice experimental liver injury in rats. Methods carbon tetrachloride (CCl4), thioacetamide (TAA), acetaminophen (AAP)-induced acute liver injury model, we determined serum ALT, AST activity. Results fallax extract on carbon tetrachloride, thioacetamide, paracetamol induced liver injury in mice significantly protective effect. Conclusion fallax extracts can significantly reduce the carbon tetrachloride,

    thioacetamide, paracetamol-induced acute liver injury model in mice serum ALT, AST activity.

     Key words fallax Protective effect of liver alanine aminotransferase aspartate aminotransferase

     Protective Effects of Polygala fallax Hemsl. On

    Experimental Liver Injury

     WANG Xiao li, HUANG Zhen, JIANG Li xia, FAN Xiao

    na, ZENG Jing

     1.Gannan Medical College, Ganzhou, 341000, China;

     2.First Affiliated Hospital of Gannan Medical College, Ganzhou, 341000, China

     Abstract: ObjectiveTo study the protective effect of

    Polygala fallax Hemsl. On experimental liver injury.MethodsThe activity of serum ALT and AST were measured in the model mice of liver injury induced by carbon tetrachloride (CCl4), acetaminophen (AAP) and thioacetamide (TAA) . ResultsThe serum

    ALT and AST activities were significantly lowered in the model mice of liver injure induced by CCl4 and AAP and TAA after

    Polygala fallax Hemsl. was given orally.ConclusionPolygala fallax Hemsl. exhibits protective effect on experimental liver injury.

     Key words: Polygala fallax Hemsl.; Protective effect on liver; ALT; AST

     Polygala fallax Polygala fallax Hemsl. (PFH) [1] also known as the fake yellow Polygalaceae, the main produced in Hunan, Guangxi, Jiangxi, Guangdong and Yunnan and other

    places, medicinal its wholly-grass. Sweet, slightly bitter

    flavor, sexual level. With replenishing, strong, dampness, Sanyu efficacy, attending virtual weak swelling, acute and chronic hepatitis, waist, leg pain, bruises, [2]. This paper reports fallax extracts on mice experimental liver injury in rats.

     1 Materials

     1.1 Drugs

     Fallax 1.1.1 Preparation of extracts obtained dry fallax (purchased from pharmaceutical companies Ganzhou) 200 g, with double distilled water than surface 2 cm, boiled 2 times. 30

    min / times, combined filtrate, concentrated to 400 ml. Plus three times the amount of 95% ethanol, Fang Bingxiang put it aside overnight, the next day vacuum filtration, and recycling the filtrate of ethanol, to continue to concentrate the

    filtrate was non-alcoholic flavored extract. Extract added to 200 ml double-distilled water to make liquid as equivalent to crude drug 1 g / ml. With 0.5% NaOH to adjust pH 7.0. Home refrigerator spare.

     1.1.2 Drug carbon tetrachloride, Shanghai and the Yangtze

    River Chemical Plant, batch number 84003; thioacetamide, Tao Bay chemical plant, batch number 881,219; paracetamol, Shantou stone Pharmaceutical Factory, batch number 040,304.

     1.2 Animal Health adult Kunming mice (from the hospital

    Medical Experimental Animal Center), evenly divided male and female, weighing 20 ~ 25 g, were randomly grouped.

     1.3 equipment made in Japan, Hitachi -7.06 thousand-

    automatic biochemical analyzer (HITACHI). Reposted elsewhere

in the paper for free download http://

     Two methods [3 ~ 5]

     2.1 fallax extract on carbon tetrachloride-induced liver

    injury in mice 32, were randomly divided into four groups, each day ig3.0, 5.0 g / kg extract of Polygala fallax or the same volume of of saline, a total of 9 d. 1 h after the last

    dose, treatment group by 10 ml / kg ip 0.1% CCl4 peanut oil solution ip control group, with the volume of peanut oil solution, after 16 h fasting, blood serum separation, serum alanine aminotransferase (ALT) , serum aspartate

    aminotransferase (AST) activity. The results in Table 1. Table 1 PFH on carbon tetrachloride-induced liver injury in rats

    (omitted)

     2.2 fallax extract on paracetamol-induced liver injury in

    mice 32, were randomly divided into four groups a day, ig

    3.0,5.0 g / kg extract of Polygala fallax or the same volume of double-distilled water , a total of 9 d. 24 h after the last administration, the treatment group by 0.150 g / kg ip acetaminophen solution, the control group the same volume of saline solution ip, 16 h after fasting, blood serum

    separation, serum ALT, AST activity. The results in Table 2. Table 2 PFH on paracetamol-induced liver injury in rats

    (omitted)

     2.3 fallax extract on thioacetamide-induced liver injury

    in mice 32, were randomly divided into four groups a day, ig

    3.0,5.0 g / kg extract of Polygala fallax solution or the same volume of double-distilled water, a total of 9 d. 1h after the last administration, the treatment group by 0.05 g / kg ip thioacetamide solution, the control group the same volume of

    saline solution ip, 16 h after fasting, blood serum separation, serum ALT, AST activity . The results in Table 3. Table 3 PFH on thioacetamide-induced liver injury in rats

    (omitted)

     3 Results

     3.1 fallax extract on carbon tetrachloride-induced liver

    injury in rats fallax extract on carbon tetrachloride-induced

    liver injury in serum ALT, AST activity increased with the apparent antagonism.

     3.2 fallax extract on paracetamol-induced liver injury in

    rats fallax extracts can be significantly reduced

    acetaminophen-induced liver injury in serum ALT, AST activity.

     3.3 fallax extract on thioacetamide-induced liver injury

    in rats fallax extracts can significantly reduce the thioacetamide-induced liver injury in serum ALT, AST activity.

     3 Discussion

     Carbon tetrachloride, paracetamol, thioacetamide liver injury in the biochemical mechanism. Is generally believed that the mechanism of carbon tetrachloride carbon tetrachloride liver injury by hepatic cytochrome P450

    metabolic activation generates trichloromethyl (* CCl3) free radicals, which can cause membrane lipid peroxidation, resulting in membrane structure and function of damage, protein substances such as anabolic place barriers, thioacetamide liver cell injury mechanisms may be damaged cell membrane structure and function. Large doses of paracetamol metabolism by the hepatic cytochrome P450 after the semi-

    quinone free radicals generated by excessive consumption of GSH to the liver and liver cells with covalently bound

    proteins, causing liver cell necrosis. These results suggest that water extract of Polygala fallax pairs of carbon tetrachloride, paracetamol, thioacetamide-induced acute liver

    injury were significantly protective effect, enabling acute liver injury in mice serum ALT, AST activity significantly reduced, with significant hepatoprotective effect. Note Polygala fallax water extract of trichloromethyl (* CCl3), semi-quinone free radicals, lipid peroxidation caused by biofilm, resulting in membrane structure and function of

    damage, protein substances such as anabolic obstacles caused by liver cells, necrosis of a protective effect.

     References

     [1] Chen Shu-kun. Chinese classification of the genus Polygala study [J]. Acta Phytotaxonomica Sinica, 1991,29 (3): 193.

     [2] Jiangsu New Medical College. Dictionary of Chinese medicine, the next book [M]. Shanghai: Shanghai Science and

    Technology Press, 1986:2079.

     [3] Qi Xue, Xiu-Mei Liu, Chen Xiaoguang, et al. Pilose antler oral liquid on mice experimental liver injury in rats [J]. Traditional Chinese Medicine and Clinical Pharmacology, 2004,20 (1): 42.

     [4] Wang Xiaodong, Zhao Ning, PENG Ling, et al. Guan Youbo prescription formulations hepatitis Kang granule on experimental liver injury in the role of research [J]. Traditional Chinese Medicine and Clinical Pharmacology, 2004,20 (1): 38.

     [5] Zhao, Peter Hills Yu, Li Bin, et al. Saponins on ethanol-induced liver injury in rats [J]. Chinese Clinical Rehabilitation, 2004,8 (30): 6686. Reposted elsewhere in the paper for free Download Center http://

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