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Fallax extracts on experimental gastric mucosal injury in rats_307

By Tony Morales,2014-10-30 17:42
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Fallax extracts on experimental gastric mucosal injury in rats_307

Fallax extracts on experimental gastric mucosal injury in rats

     Abstract Objective To study the extract of Polygala fallax gastric mucosal injury in mice. Method of anhydrous ethanol, aspirin, France, flooding stress law and reserpine

    Preparation of gastric mucosal injury model in mice were determined after administration area of gastric mucosal injury. Results fallax extracts four kinds of gastric mucosal damage were significantly inhibited. Conclusion Polygala fallax extracts on experimental gastric mucosal injury in a clear protective effect.

     Key words gastric mucosal injury in mice fallax

     Protective Effect of Polygala fallax Hemsl. On Experimental Gastric Mucosal Lesion

     Abstract: ObjectiveTo observe the mucosal protective

    effect of Polygala fallax Hemsl.in experimental model of gastric lesion in mice.MethodsThe four models of gastric mucosal lesion in mice induced by ethanol absolute, aspirin, reserpine injection and water immersion stress were established.The areas of gastric mucosal lesion in mice were measured.ResultsIt was found that Polygala fallax Hemsl. obviously inhibited lesion degrees of gastric mucosa of the four models.ConclusionPolygala fallax Hemsl. possesses protective effect on experimental gastric mucosal lesion in

    mice.

     Key words: Polygala fallax Hemsl; Gastric mucosal lesion; Mice

     Polygala fallax Polygala fallax Hemsl. (PFH) [1] also known as the fake yellow Polygalaceae, the main produced in Hunan, Guangxi, Jiangxi, Guangdong and Yunnan and other

    places, medicinal its wholly-grass. Sweet, slightly bitter

    flavor, sexual level. With replenishing, strong, dampness, Sanyu efficacy, attending virtual weak swelling, acute and chronic hepatitis, waist, leg pain, bruises, [2]. Some people

    have been reported fallax extracts on immune organs [3]. Are reported fallax extracts on experimental gastric mucosal injury in rats.

     1 Materials

     1.1 Animals Kunming mice, male and female in half, body weight (22.0 ? 1.5) g, from Jiangxi Medical College

    Laboratory Animal Center, Animal qualified证号: 2006

     075. Randomized. In the laboratory before the experiment to adapt to 1 week after the experiment.

     1.2 Drugs and reagents to take dry water fallax (purchased from pharmaceutical companies Ganzhou) 200 g, with

    double distilled water than surface 2 cm, boiled 2 times. 30 min / times, combined filtrate, concentrated to 400 ml. Plus three times the amount of 95% ethanol, Fang Bingxiang put it aside overnight, the next day vacuum filtration, and recycling

    the filtrate of ethanol, to continue to concentrate the filtrate was non-alcoholic flavored extract. Extract with double distilled water to 200 ml, so that liquid is the equivalent of crude drug 1 g / ml. With 0.5% NaOH to adjust pH 7.0. Home refrigerator spare.

     Cimetidine injection, Xuzhou Ryan Pharmaceutical Ltd., batch number 050,404. Anhydrous ethanol, Jiangxi Hongdu Biochemical Co., Ltd. production, batch number 051,201. Aspirin, Beijing Pacific Pharmaceutical Co., Ltd. production,

    batch number 050,301. Reserpine injection, Shanghai Fudan Fuhua Pharmaceutical Co., Ltd. production, batch number 050201.

     2 method [4 ~ 8]

     2.1 The ethanol-induced gastric mucosal injury model in mice prepared by taking 40 Kunming mice were randomly divided

    into 5 groups: normal saline control group, model group,

cimetidine group fallax extracts of low-dose group, Polygala

    fallax high-dose group. Each gavage 1 / d, a continuous 7 d. Gavage 7 days after fasting 24 h, can not help but water. On

    the 8th day after the last dose 1 h, in addition to normal saline control group, mice in each group were fed ethanol (0.1 ml / only), 1h after cervical dislocation of mice were sacrificed.

     2.2 of aspirin-induced gastric mucosal injury model in

    mice Preparation of animal grouping and drug delivery methods are the same before. At 1 h after the last dose, in addition to normal saline control group, each group of mice fed aspirin 0.25 g / kg, 4 h after the mice were sacrificed.

     2.3 Flooding stress-induced gastric mucosal injury model

    in mice Preparation of animal grouping and drug delivery methods are the same before. 1h after the last administration, in addition to normal saline control group, each group of mice under ether anesthesia at the iron bars on the mice bondage

    placed (23 ? 0.5) ? in water bath, the liquid level to keep the sword in the sternum sudden level. 7 h after the mice were sacrificed.

     2.4 reserpine-induced gastric mucosal injury model in

    mice Preparation of animal grouping and drug delivery methods

    are the same before. At 1 h after the last dose, in addition to normal saline control group, the subcutaneous injection of 10 mg / kg reserpine, 6 h after the mice were sacrificed. Reposted elsewhere in the paper for free download http://

     The experimental animals were killed after the Cesarean section, separating the stomach, gastric cardia and pyloric ligation to the stomach cavity injection of 10% formaldehyde solution, 2 ml, will be immersed in the stomach in the same

    concentration of formaldehyde solution, 20 min after the cut along the greater curvature of stomach appetizer, Water rushed gastric residue, expand the stomach and placed under a microscope, observe the degree of gastric mucosal damage, and calculated on the grid area of gastric mucosal damage to each area of gastric mucosal damage in mice (mm2) of the sum of its damage index . Ulcer index, calculated as follows: ulcer points, or 1 mm below surface, namely, 1 minute; 1 ~ 2 mm were, namely, two points; 2 ~ 3 mm were, namely, 3 points; 3 ~

    4 mm were, namely, four points; more than 4 mm were, namely,

    five points shall be added to the scoring of the animals of the ulcer index, and calculate the inhibitory rate of ulcers.

     3 Results

     3.1 fallax extract on ethanol induced gastric mucosal injury in rats results show that different doses of extract of Polygala fallax index of gastric mucosal damage in mice compared with the model group were significant differences. The results in Table 1. Table 1 fallax extracts on ethanol

    induced gastric mucosal injury in rats (omitted)

     3.2 fallax extracts on aspirin-induced gastric mucosal

    injury in mice showed that different doses of extract of Polygala fallax gavage of aspirin caused gastric mucosal injury were significantly protective effect of . The results in Table 2. Table 2 fallax extracts on aspirin induced gastric mucosal injury in rats (omitted)

     3.3 fallax extracts on water immersion stress-induced

    gastric mucosal injury in mice of different doses of Polygala

    fallax extracts on water immersion stress-induced gastric

    mucosal injury significantly inhibited. The results in Table 3. Table 3 fallax extracts on water immersion stress induced gastric mucosal injury in rats (omitted)

     3.4 fallax extract on reserpine-induced gastric mucosal

    injury in different dose groups extract of Polygala fallax on the reserpine-induced gastric mucosal injury in mice

    significantly inhibited. The results in Table 4. Table 4 fallax extract on reserpine-induced gastric mucosal injury in

    rats (omitted)

     4 Discussion

     Current gastric ulcer formation due to a combination interpretation of the theory has not yet formed a positive cause. The pathogenesis of ulcer disease may be due to gastric acid and pepsin digestion ability and the balance between the gastric mucosal barrier function disorder. Mental or emotional, stress, drugs and diet are caused by ulcers incentives. Based on available chemical, physical stimulation as well as through a variety of stress-induced ulcers

    pathological model. In this study, a variety of animal models

    of gastric ulcer, from different angles of Polygala fallax extracts on gastric mucosal protective effect. The results showed that fallax extracts significantly reduced by ethanol, aspirin, flooding stress and reserpine induced gastric mucosal injury, showed significantly reduced damage index, ulcer inhibitory rate increased significantly. Tips fallax extracts on a variety of models of experimental gastric mucosal injury caused by both a protective effect, these results for clinical for the treatment of peptic ulcer provides a theoretical basis.

     References

     [1] Chen Shu-kun. Chinese classification of the genus Polygala study [J]. Acta Phytotaxonomica Sinica, 1991,29 (3): 193.

     [2] Jiangsu New Medical College. Dictionary of Chinese medicine, the next book [M]. Shanghai: Shanghai Science and Technology Press, 1986:2079.

     [3] He Yong, LI Hong-liang, British Columbia accounted

    for Yu, et al. Fallax extracts on immune effects of [J].

    Gannan Medical College, 2006,26 (6): 827.

     [4] Xiao-Mian Zhou, Chenggang, Xu Beibei, et al. WKN main pharmacodynamics [J]. Journal of Shenyang Pharmaceutical University, 2002,19 (5): 344.

     [5] Guo-Qing Wang, Li Xiaomei, Yang Bo, et al. Magaldrate preliminary study of anti-ulcer effects [J]. Journal of

    Shenyang Pharmaceutical University, 2000,17 (3): 203.

     [6] Zhao Rumei, Du Jinsong, Geng Dongsheng, et al. Well-

    being of oral liquid on mice gastric ulcers Pharmacodynamic Studies [J]. PLA Pharmaceutical Sinica, 2000,16 (4): 178.

     [7] Xiao-Li Liu, Chun-Xia Liu, Ju-yuan, et al. Pipemidic

    zinc on experimental gastric ulcer protective effect and its mechanism [J]. Chinese Journal of Clinical Pharmacology and

    Therapeutics, 2002,7 (2): 127.

     [8] Zhao, the Chen Zhiwu, Bi-Wei Song, et al. Hyperin on

    gastric mucosal injury in mice and its mechanism [J]. Anhui Medical University, 1999,34 (3): 178. Reposted elsewhere in the paper for free download Center http://

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