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Enterobacter cloacae AmpC enzyme detection of high-yield and molecular epidemiological study_3036

By Tammy Murphy,2014-10-30 17:02
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Enterobacter cloacae AmpC enzyme detection of high-yield and molecular epidemiological study_3036

Enterobacter cloacae AmpC enzyme detection of high-yield and

    molecular epidemiological study

     Author: Huda Chun Shao Jian-chun Yang Shaomin Li

    Chao Ping \

     Abstract Objective To study the hospital Enterobacter cloacae AmpC enzyme and molecular epidemiological

    characteristics of high-yield. Methods The KB method

    susceptibility testing, double disk synergy test and enzyme extract high-yielding three-dimensional test for detection of

    Enterobacter cloacae AmpC enzyme, polymerase chain reaction

    for detection of AmpC enzyme, ERIC repeat rep PCR, Research

    Kunming First People's the hospital in July 2002 ~ December 2004 of 84 clinical isolates of Enterobacter cloacae resistant, AmpC enzyme genotype and clone spread of conditions. Results ESBLs phenotype screening test and confirmatory test, high-AmpC enzyme detection rate of 27.38% (23/84), ESBLs detection rate of 16.67% (14/84), while high-

    yield AmpC enzymes and ESBLs detection rate of 44.05% ( 37/84). AmpC gene detection rate of 43.75% (28/64). AmpC-

    dimensional test yielding a detection rate of 47.62% (40/84). Cloning communication analysis and found that strain No. EC2 and EC11, EC13 and EC14, EC19 and EC20, respectively have the same DNA fingerprint and the remaining no DNA fingerprints

    between strains. Conclusion Enterobacter cloacae resistance is more complicated. Go out to deter the performance of high-

    yielding AmpC enzyme, producing ESBLs, simultaneous production of AmpC enzymes and ESBLs and plasmid AmpC enzyme multi-drug-

    resistant phenotype. Molecular epidemiology revealed that, although the spread of Enterobacter cloacae and antibiotic induction and selection of the role of resistance in patients with decreased body and gut dwelling bacteria infection of endogenous factors, but still want to guard against the

occurrence of hospital infection.

     Key words Enterobacter cloacae AmpC β lactamase enzyme

    extracts from three-dimensional test re-order sequence primed

    polymerase chain reaction (ERIC rep PCR)

     Gene detection and the molecular epidemiology of Enterobacter cloacae

     with hyperproduction of AmpC β lactamase

     ABSTRACT Objective To investigate the molecular epidemiology of Enterobacter cloacae with hyperproduction of AmpC β lactamase. Methods Using the Kirby Bauer (KB)

    antibiotic susceptibility test, cloxacillin synergistic

    disc diffusion and the modified three dimensional

    extraction test, E.cloacae hyperproducing AmpC β lactamase

    were examined. The differential genotypes of AmpC β

    lactamase were investigated using the Enterobacter repetitive intergenic consensus (ERIC) for repetitive extragenic palindromic element based PCR (rep PCR). The study was

    carried on in the First People's Hospital of Kunming from July, 2002 to December, 2004 in 84 clinically separate cases of E.cloacae. We determined the drug resistance profile,

    genotype and molecular epidemiology of each strain. Results Using phenotypic screening and extended spectrum β

    lactamases (ESBLs ) confirmatory test, twenty three strains

    (23/84, 27.38%) of E.cloacae were found to have hyperproduction of AmpC β lactamase. The rate of ESBLs

    positive samples was 16.67% (14/84) and 44.05% had elevated AmpC β lactamase and ESBLs (37/84). The rate of AmpC β

    lactamase gene detection was 43.75% (28/64). Using the three

     dimensional extract test, we observed AmpC β