Ephedra fruit polysaccharides in rabbit ventricular papillary myoelectric activity of
【Abstract】 Objective To observe the Ephedra fruit
polysaccharide on isolated rabbit ventricular papillary muscle action potentials, and to explore its mechanism. Methods glass
microelectrode method of intracellular records, trace recorded ephedra fruit polysaccharides on ventricular papillary muscle resting potential (RP), action potential amplitude (APA), 0 maximal depolarization velocity (Vmax), re-extreme 50 % and
90% (APD50, APD90) of the time of impact and the use of M receptor blocker and β-receptor blocker, to explore the role of the target. The results of ephedra, when too much sugar, 5 min reperfusion, APD, APD50, APD90 was significantly prolonged
gradually after shortening; 10 min, the three indicators and significantly shorter than the control group. After the application of M receptor blocker, the effect is gone; application of β-receptor blocker, the first post-shortening
effect of the extension still exist. Conclusion Ephedra fruit polysaccharides acting on the ventricular papillary muscle M receptor inhibition of K channels are the first post-
facilitated two-way effect.
Key words Ephedra fruit polysaccharides ventricular papillary
muscle action potential
Effect of Ephedra vulgans Fruit Polysaccharide (EVFP) on Electric Potential of Ventricular Papillary Muscle in Rabbits
AbstractObjectiveTo observe the effects and the mechanism of EVFP on electric potential of ventricular papillary muscle
in rabbits. MethodsThe electric potentials of isolated ventricular papillary muscle were intracellularly recorded. Electrophysiological parameters were: resting potential (RP),
amplitude of action potential (APA), maximal rate of depolarization of phase 0 (Vmax), duration of action potential (APD), duration of 50% and 90% depolarization (APD50 and APD90). The effects and mechanisms of action EVFP on the electric potentials were observed. ResultsWhen 120 μmol EVFP
had been reperfused for 5 min, the APD, APD50, and APD90 were prolonged, and then they were shorten gradually.Compared with the controt group, the three indexes were obviously shortened when EVFP reperfused for 10 min. These effects disappeared when block agent of M-receptor was used before EVFP
reperfusion. But these effects existed when block agent of β-
receptor was used before EVFP reperfusion.ConclusionAPD, APD50 and APD90 of ventricular papillary muscle were prolonged by EVFP. The effect may be related to M-receptor.
Key wordsEphedra vulgans fruit polysaccharide; Ventricular papillary muscle; Electric potential
Division of ephedra ephedra plants, with sweating solution table, Xuanfei asthma, swelling and other diuretic effect. Ephedra Ephedra is the fruit of fruit polysaccharide
in one of the main active ingredient, you can inside and outside the source of two ways to play its anticoagulant effect, but also the role of M-receptors in the myocardium,
there is a clear antihypertensive effect. To observe the Ephedra fruit polysaccharide on isolated rabbit ventricular papillary muscle action potential, and to explore its mechanism, in order to ephedra fruit polysaccharides in the theoretical basis for clinical applications.
1 Materials and methods
1.1 Drug taking ephedra extract says its fruit each 4 g, Split Round-bottom flask, add 35 ml of water boiling reflux 2 h, filtration, residue of water and then back twice, 20 ml / second, the merger filtrate, too big DA101 macroporous resin column bleaching, receive fluid solution by adding 5% ZnSO4 and saturated Ba (OH) 2 solution to precipitate the protein amount, Zhen Yao, put it aside, take supernatant dropping Ba (OH) 2 until no precipitation generation, filtering to 100 ml flask, add water, diluted to the scale .
1.2 Specimen Preparation 15 rabbits, male and female informal, weight (2.0 ? 0.5) kg. Head to the stunning blow,
speed to take the heart, placed in O2 saturated Lock solution, coronary washed, cut the right ventricle, whichever is papillary muscle. Papillary muscle fixed in the perfusion tank of silicone rubber, and with the modified Locke solution to 10 ml / min for constant speed perfusion maintained at a temperature (36 ? 1) ?, perfusion fluid filled with pure O2, pH 7.4. Stability of specimens in the perfusion fluid 30 min after the experiment.
1.3 Potential lead glass microelectrode DC resistance 10 ~ 20 MΩ. Given pulse width 2 ms, 1 Hz, twice at the threshold intensity of the square wave stimulation, potential stabilized to start the experiment. Action potentials by DWF-3 micro-
electrode amplifier amplification, all the way type SBR-1-type
oscilloscope for observation, all the way input listener listen, and the other passing high-speed analog input
computer, automatically signals, and analyze the electrical
action All parameters of bit.
1.4 Observation resting potential (resting potential, RP), action potential amplitude (amplitude of action potential, APA), 0 maximal depolarization rate (maximal rate of depolarization, Vmax), action potential duration (duration of action potential, APD), re-extreme 50% and 90% of the time
(duration of 50% and 90% repolarization, APD50 and APD90).
1.5 experiment to be stable action potentials, gathering a group of normal action potential as the control, and then
switch to other perfusate perfusion, respectively, recorded 5 min and 10 min of action potential changes in the final wash with a new Lock observe the action potential recovery process. Reposted elsewhere in the paper for free download http://
1.6 Packet ? pure ephedrine fruit polysaccharide
reperfusion group (Ephedra fruit polysaccharide group); ?
mere 10 μmol / L acetylcholine reperfusion group (Ach group); ? M receptor antagonist atropine, ephedrine fruit polysaccharide group (Atto Ephedra products fruit
polysaccharide group) (first trace recorded after a group of normal action potentials, with 10μmol / L atropine, 5min
perfusion, after perfusion with ephedra fruit
polysaccharides); ? β-blocker timolol Ephedra liquid over
fruit glucose group (timolol ephedra fruit polysaccharide group) (describe action potentials recorded after a group of normal, with 10 μmol / L timolol 5min perfusion, after
perfusion with ephedra fruit polysaccharides).
1.7 statistical processing application Excel statistical
tools, action potential indicators for each observation are ?
s, said to the front and rear elements of the indicators used t tests to P
2.1 Ephedra fruit polysaccharides right ventricular papillary muscle action potentials of 120 μmol / l ephedrine
perfused 5 min when the fruit polysaccharides, APD, APD50 and APD90 was significantly prolonged, with the control group had significant difference. 6 ~ 7 min followed by perfusion began to shorten, 10 min, when stabilized, APD, APD50 and APD90 were significantly shorter than the normal control group, followed by no significant change. The remainder showed no significant changes observed. Rinse after 10 min restored to normal levels. The results in Table 1. Table 1 Ephedra fruit
polysaccharides in rabbit ventricular papillary muscle action potentials (omitted)
2.2 of acetylcholine on ventricular papillary muscle action potentials of 10 μmol / L Ach perfused 5 min, the
action potential APD, APD50 and APD90 significantly shortened, after no significant changes until 10 min when the perfusion. Rinse after 10 min restored to normal levels. The results in Table 2. Table 2 Effect of acetylcholine on rabbit ventricular papillary muscle action potentials (omitted)
Atropine 2.3 Ephedra fruit polysaccharides on ventricular papillary muscle action potentials of 10 μmol / L of atropine
perfused 5 min, the action potential no significant change in the indicators. With 120 μmol / L ephedra fruit
polysaccharides action potentials when perfused 5 min each index as compared with normal controls no significant changes,
10 min, remains stable at the same level. Rinse after 10 min still no significant changes. The results in Table 3. Table 3 atropine, ephedrine fruit polysaccharides on rabbit ventricular papillary muscle action potentials (omitted)
Ephedra timolol 2.4 fruit polysaccharides on ventricular papillary muscle action potentials of 10 μmol / l timolol 5
min perfusion, the action potential of the indicators change significantly. With 120 μmol / l ephedrine perfused 5 min
when the fruit polysaccharides, APD, APD50 and APD90 was significantly prolonged, compared with the normal control group were significantly different. Followed by perfusion after 6 ~ 7 min progressively shorter, 10 min, when stabilized, APD, APD50 and APD90 were significantly shorter
than the normal control group, followed by no significant change. The remaining observations showed no significant change. Rinse after 10 min restored to normal levels. The results in Table 4. Table 4 timolol Ephedra fruit polysaccharides on rabbit ventricular papillary muscle action potentials (omitted)
The biological activity of plant polysaccharides have been more and more cause for concern, and its anticoagulant, lowering blood sugar, enhance immunity and so on have been
more research [2 ~ 4]. Ephedra Ephedra fruit polysaccharide is a traditional Chinese medicine the fruit is one of the main active ingredient, which can also be inside and outside the source of two ways to play its anticoagulant effect , we have in the overall study also found that in rabbits, and whose role M-receptors in the myocardium, there is a clear antihypertensive effect .
This experiment, pure fruit polysaccharides Ephedra perfused 5 min, significantly prolonged action potential
duration, APD50 and APD90 were prolonged, that the outflow diminished K repolarization phase; 6 ~ 7min when the opposite result, action potential duration significantly shortening; APD50 and APD90 were shortened, 10 min, when stable at the level of APD shortening, indicating that increased outflow of K repolarization phase. Application of M receptor antagonist atropine, after perfusion, and then perfusion with the same concentration of ephedra fruit polysaccharides is hardly the above-mentioned phenomenon shows that ephedra fruit
polysaccharides on the electrical activity of cardiac effects of the role of this receptor, resulting in M . Application of β-blocker timolol, after perfusion, and then fruit polysaccharides ephedra perfused 5 min and at 10 min when the
action potential duration of the performance and a simple application similar to ephedra, when fruit polysaccharides, indicating that the role of ephedra Fruit Polysaccharide in the M receptor, its effects are not exactly the same effect of acetylcholine on K controls the outflow of a post-facilitated
two-way effect, due to the start of the inhibitory effect of a shorter, while the strong facilitatory effect to K outflow acceleration, action potential duration shortened, so that myocardial cell excitability, conduction is reduced, due to K outflow acceleration, can inhibit the cardiac cell membrane permeability for Ca2, so that Ca2 flow reduced, thus reducing the myocardial contractile force, which our previous overall The mechanism of antihypertensive effect of the experiment the
To sum up, ephedra fruit polysaccharides could act on the isolated ventricular papillary muscle M receptors, ion channels are the first K right after the facilitatory inhibitory effect of both, thereby affecting the electrical
activity of the heart muscle.
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