Epimedium total flavonoids extraction method
【Abstract】 Objective: To study the extract of Epimedium total flavonoids choose the best way to study the ultrasound method, supercritical extraction, semi-bionic extraction,
Soxhlet extraction, reflux, pot Brewing decoction extract the six extraction methods Epimedium total flavonoids extraction efficiency. Methods: 754 samples spectrophotometer of flavonoids. Results: The Soxhlet extraction was the highest content of total flavonoids. Conclusion: The ultrasonic
extraction method is suitable extraction method.
Key words total flavone of Epimedium
A study on the Extractive Technology of Total Flavone from Herba Epimedii
【Abstract】 Objective: To study four technological
process, ultrasonic wave extraction, soxhlet'S extractor, circumfluence, and water extraction, a better technological condition to extracting gogal flavone from Herba Epimedii was obtained.Methods: By using the 754 type's UV
spectrophotometer, analyzing the content of total flavone of
each sample.Results: Using soxhlet's extractor, the content of total flavone is the highest among four technological process.Concusion: A better technological condition to extracting total flavone from Herba Epimedii is ultrasonic
【Key words】 Herba Epimedii total flavone extract
Epimedium (Epimedium brvicornum Maxim) is a Berberidaceae
plants warm in nature, sweet Sim, owned by the liver, kidney, clinically commonly used in impotence and nocturnal emission, limp muscles, rheumatism Bitong, numbness Juluan and
climacteric hypertension . Elements of modern chemistry studies have shown that the main effective ingredient of Epimedium Epimedium flavone (icariin, epimedium sub-
glucoside), and epimedium polysaccharide, the total flavonoids
with increase cerebral blood flow and coronary blood flow , anti-tumor, anti-oxidation and enhance the body's immune
system and other functions . In order to select the exact method, we carried out with 70% ethanol ultrasonic method, supercritical extraction, Soxhlet extraction, reflux, semi-
bionic extraction of the comparative study conducted at the same time pot Brewing decoction of the comparative experiment, from which to find accurate, simple and fast extraction method.
An experimental material
1.1 Instrument 754 UV grating spectrophotometer (Shanghai Third Analysis Instrument Factory), TG328A (s)-type analytical
balance (Shanghai Balance Factory refined Branch), PHS-3C
precision pH meter Model (Shanghai electro-optic devices
Factory), HA121-50 -01 Supercritical Fluid Extraction Device (SFE Nantong Hua Co., Ltd.).
1.2 Drugs Epimedium (purchased in the Yellow River medicine market) by the Gansu College of Health and Medicine Teaching and Research was identified as plant epimedium;
icariin standard (purchased in China's pharmaceutical and biological products).
2 Experimental Methods and Results
2.1 Preparation of standard curve Precision Weigh dried to constant weight icariin standard 4.6mg buy 25ml flask in
constant volume of 70% ethanol to the scale. And then draw 0.5,1.0,1.5,2.0,2.5 ml were placed in 25ml volumetric flask, respectively, constant volume of 70% ethanol to the scale. With 70% ethanol as the gaps in absorbance measured at 270nm in order to absorb the value of (A) for the vertical axis, the concentration (C) as the abscissa, drawing standard curve. To the data for statistical processing, were regression equation: A = 0.049270C +0.039796, r = 0.9991, linear range of 3.7 ~
2.2.1 Ultrasonic Extraction to take 1g of Epimedium meal
plus 70% ethanol 20ml, ultrasonic extraction 1h, extract filtration, placed in 25ml volumetric flask, and with 70% ethanol to 25ml flask in the constant volume, and then draw 2ml to a 10ml volumetric flask in constant volume with 70% ethanol to the scale, and then learn from 1ml to 25ml flask in constant volume with 70% ethanol to the scale, shake, for determination.
2.2.2 Soxhlet Extraction taking epimedium meal 1g, placed in Soxhlet extractor, add 70% ethanol 100ml, extraction 6h, surcharge of ethanol to the dry residue was dissolved in 70% ethanol, after filtration 70 % ethanol to 25ml flask in the constant volume, and then draw 2ml into 10ml flask in constant volume with 70% ethanol to the scale, and then learn from 1ml to 25ml flask in constant volume with 70% ethanol to the scale, shake for determination.
2.2.3 reflux taking epimedium meal 1g, with 20ml 70% ethanol was extracted two times, each time 1h, merging liquid recycling ethanol to dry, with 70% ethanol dissolved in 70%
ethanol after filtration constant volume to 25ml flask, the draw 2ml into 10ml flask in constant volume with 70% ethanol to the scale and learn 1ml to 25ml flask in constant volume with 70% ethanol to the scale, shake, for determination.
2.2.4 decoction of Epimedium meal to take pot Brewing 4g, soaked in 80ml water 1.5h, boiling two times, each time 1h, combined liquid filtration, water bath, evaporated and the residue dissolved with 70% ethanol, after filtration in constant volume to 100ml flask, and then draw 2ml into 10ml
flask in constant volume with 70% ethanol to the scale, and then learn from 1ml to 25ml flask in constant volume with 70% ethanol to the scale, shake for the determination of . Reposted elsewhere in the paper for free download http://
The above experiment repeated three times each to calculate the average content of total flavonoids.
2.2.5 Semi-bionic extraction to take Epimedium meal by L9
(34) orthogonal to icariin content indicators orthogonal experiment. Combined liquid filtration, water bath, evaporated and the residue dissolved with 70% ethanol, after filtration flask in the constant volume to 25ml, and then draw 5ml to 25ml flask in constant volume with 70% ethanol to the scale, and then draw 2ml to 10ml flask in constant volume with 70%
ethanol to the scale, and then learn from 1ml to 25ml flask in constant volume with 70% ethanol, shaken to the scale for the determination.
2.2.6 Supercritical Fluid Extraction to take off on the 3rd screen of Epimedium powder and precision weighing 200g,
according to Table 4, experiment, extracts water bath, evaporated and the residue dissolved with 70% ethanol, after filtration constant volume to 100ml Flask in, and then draw 0.5ml to 25ml flask in constant volume with 70% ethanol to the
scale, and then draw 2ml into 10ml flask in constant volume with 70% ethanol to the scale, and then learn from 1ml to 25ml volumetric flask, by using 70 % ethanol will allow to scale shake for determination.
2.3 Determination of the samples
Were collected from the above extract, to 70% ethanol as a blank, 270nm for the determination of the wavelength, the absorption was measured under test the value of hydraulic pressure. The measured absorbance A substituted into the regression equation to calculate the concentration of sample solution, and converted into percentage content of the sample shown in Table 1. Table 1 Effect of different extraction methods of total flavonoids Note: The semi-bionic extraction
and supercritical fluid extraction, the average levels were the highest value obtained.
2.4 Semi-bionic extraction of orthogonal experimental design
According to SBE law theory [3 ~ 6], in the medicinal granularity, fried to mention temperature, add boiling water,
concentrated under the premise of the same conditions to determine the main factors and level of study. Selected L9 (34) Table arrangements for pilot program, shown in Table 2, Table 3, Table 4. Table 2 factors - the level of the table
Note: The decoction three times longer than the 2:1: 1, add
water ratio 20:12:12. Table 3 orthogonal L9 (34) the results of analysis of typical examples of analysis of variance in Table 4 Note: The extraction time 1.5h, separation of the error! Reference source not found. Pressure 8Mp, separation of
the error! Reference source not found. Pressure 4Mp, cosolvent of 95% ethanol.
3.1 to icariin content indexes from tables 3 and 4 we can see that R value is very small due to B factors, can be used as error term, A, C, D factors are very significant differences in the impact of icariin factors, primary and secondary factors for A> C> D> B. Intuitive results of the analysis shows that the best extraction composition A1C2D1B2. Verification experiment further indicates that the process is
stable. Therefore, the best extraction was screened as follows: first Jianshui PH value (2.00), the second Jianshui PH value (7.00), third Jianshui PH value (8.50), boiling time of 3h.
3.2 from Table 5 we can see, supercritical extraction of
the extraction efficiency is low, the reason may be related to the following factors: experimental period is short, experimental and limited funds, so that the experiments need to be further optimized experimental conditions.
3.3 From the above results and intuitive analysis, the six extraction methods. Total flavonoids from high to low were as follows: Soxhlet extraction Law "ethanol reflux extraction Law" Ultrasonic Extraction of Law "semi-bionic extraction Law"
decoction pot Brewing "supercritical fluid extraction method; extraction time from high to low were as follows: SUO s extraction Law "semi-bionic extraction Law" decoction pot
Brewing "extract ethanol reflux Law" Supercritical Fluid Extraction Law "ultrasonic extraction method.
In summary, the ultrasonic extraction of the shortest extraction time, higher levels of total flavonoids, extraction, high efficiency, simple, rapid, and as the best extraction method.
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