Emei different altitude populations of quercetin content in Houttuynia_477

By Rodney Henderson,2014-10-30 16:34
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Emei different altitude populations of quercetin content in Houttuynia_477

    Emei different altitude populations of quercetin content in Houttuynia

     Author: Liu Lei, Wu Wei, Zheng Liang, Sandra Oh

     Abstract Objective To study the Emeishan populations at different altitudes Yuxingcao differences in quercetin

    content. Methods Hypersil ODS C18 (5 μm, 4.0 mm × 250 mm)

    column, mobile phase of acetonitrile: 0.4% phosphoric acid = 45:55, adjusted pH = 3, flow rate 1.0 ml / min, column temperature 30 ?, UV detection wavelength of 370 nm. Results The method is simple, rapid, accurate, and can be used as Yuxingcao quantitative analysis of quercetin. Conclusion of 9 different elevation populations of quercetin Houttuynia there are significant differences in the levels and altitude and the correlation between soil nutrient content are not significant.

     Key words Houttuynia; quercetin; altitude; soil nutrients; RP-HPLC

     Abstract: ObjectiveTo study the difference of quercetin content of H.cordata Thunb. From different altitudes of Mt.Emei.MethodsQuercetin was determined by HPLC on Hypersil ODS C18 (4.0 mm × 250 mm, 5 μm) at 30 ? with acetonitrile:

    0.4% phosphoric acid (45:55), pH = 3 as the mobile phase.Detection wavelengh was 370 nm.The flow rate was 1.0 ml / min.Results This method is simple, fast and accurate, and

    can be the quantitative analytic method of quercetin content of H. cndata.ConclusionThere are significant differences among the quercetin content of 9 populations from different altitudes.The correlationship between quercetin content and

    altitudes and soil nutrient are not significant.

     Key words: Houttuynia cordata Thunb.; Quercetin;

Altitude; Soil nutrient; RP HPLC

     Houttuynia is Saururaceae Houttuynia Houttuynia cordata Thunb. Of the whole plant. Widely distributed in central China, south-east and south-western provinces and autonomous

    regions, especially in Sichuan, Hubei, Hunan, Jiangsu and other provinces mostly. Of Weixin, cold, with heat detoxification, swelling removing the abscess, the diuretic effect of Tonglin. Houttuynia In addition to containing volatile oils, but also contains a large number of quercetin, quercitrin, iso-quercitrin and so on. Quercetin which has expectorant, relieving cough and asthma, anti-inflammatory,

    anti-allergy, expansion of coronary, blood fat, anti-

    arrhythmia, anti-platelet aggregation, anti-oxidation, anti-

    tumor a wide range of pharmacological effects [1], and their contents is a measure of an important indicator of Houttuynia medicinal value.

     Mount Emei is located in the southwest edge of Sichuan Basin, China, was formed in 3000 years ago before the

    Himalayan movement. Mountain for nearly north-south mountain

    ranges, the highest peak altitude 3 099 m. Mount Emei mild climate, clouds and more sunshine less rainfall, air relative humidity, there is a wealth of plant resources. According to

    our previous investigation, Mount Emei Yuxingcao resources are very abundant, mainly in the altitude of 500 ~ 1 800 m range.

     In recent years, the study reported that domestic Yuxingcao mainly in the production of cultivation,

    pharmacological efficacy, clinical applications, and genetic background [2 ~ 5] and so on. Some scholars of some plant secondary metabolites and the relationship between altitude studied [6,7], the majority of results showed that the sub-

    product of chemical and biological Xie and altitude there were significant or very significant correlation. Some academics have also secondary metabolites of plants and soil nutrients in the relationship between the studied [8], results showed that the soil nutrients on plant secondary metabolites of the

    synthesis is also significant. Previously, I had Emeishan populations at different altitudes Houttuynia volatile oil constituents of a research and found that part of the volatile oil composition and altitude, and soil nutrients there were

    significant correlation (ecological Sinica, to be published). This study, using RP-HPLC method were determined at different altitudes 9 Yuxingcao populations quercetin content of altitude and soil nutrients and the relationship between quercetin, through the secondary metabolites and the altitude and soil nutrients in a comprehensive evaluation of artificial cultivation of Houttuynia the appropriate choice of altitude and soil conditions and further rational development and utilization provide some theoretical basis.

     An instrument and reagent

     Agilent's 1100 High Performance Liquid Chromatography (including degasser, quaternary gradient pump, autosampler, column temperature box, DAD detector), Sartorius Corporation BP211D electronic balance, ICE's Micromax centrifuge, Millipope Company MilliQ-type water instrument.

     Chromatography pure acetonitrile by TEDIA companies, quercetin reference substance (purity "98%) from Sigma company, with the remaining reagents of analytical pure.

     Table 1 Test materials, the collection location, altitude, growing season environment and multi-degree


     2 Materials and methods

     2.1 The experimental material due to Emei Mountain for more than 1 800 m above sea level in areas find it difficult to Houttuynia collected, the testing of all populations of the materials are taken from the altitude of 1 800 m below. 9Materials and the growth of populations in soil samples in the20050713Collected from the western slope of Mount Emei. The altitude of populations, growth environment and abundance in Table 1. The identification of Sichuan

    Agricultural University, Dr. Wu Wei are Houttuynia cordata Thunb..

     2.2 The growth of Mount Emei Houttuynia the basic conditions of soil nutrient content in the growth of the Emeishan Yuxingcao soil nutrient content of the basic

    conditions in table 2. Soil sample processing and analysis of the various nutrient elements in the light of "soil agricultural chemistry analysis" in the conventional methods.

     2.3 Chromatographic conditions Column: Hypersil ODS C18 (5 μm, 4.0 mm × 250 mm); mobile phase: acetonitrile: 0.4% phosphoric acid = 45:55, adjusted pH = 3. Flow rate: 1.0 ml / min; column temperature: 30 ?; UV detection wavelength: 370

    nm; quercetin in the control and test samples for the

    chromatographic separation shown in Figure 1.

     Table 2 Growth of Mount Emei Yuxingcao soil nutrient content (abbreviated)

     Data listed in the table 3 times the average of measurement results, "/" indicates not detected due to reasons

    for the test