Effect of lycopene on urethane-induced lung effects in mice
【Abstract】 Objective To study the lycopene on urethane-
induced lung effects in mice. Ways to urethane (1 000 mg / kg) 1 in mice induced by intraperitoneal injection of lung cancer,
mice fed with lycopene for 18 weeks to observe the rate of tumor detection in serum and lung tissue superoxide dismutase ( SOD), catalase (CAT), glutathione S transferase (GST)
activity and malondialdehyde (MDA), interleukin 2 (IL 2)
and tumor necrosis factor (TNF α) levels. Results lycopene
on urethane-induced lung cancer in mice after the formation of SOD, CAT activity and IL 2 and TNF α levels have also
picked up the role, and can stop with the corresponding GST activity in cancerous and the MDA content increased, reducing the incidence of cancer . Conclusion of lycopene on urethane-
induced lung cancer in mice have a preventive effect, the main mechanism may be related to antioxidant and immunomodulatory effects.
【Key Words】 Lycopene; urethane; lung cancer
Effect of Lycopene on Lung Carcinogenesis Induced by Urethane
Abstract: ObjectiveTo study the effect of lycopene on lung carcinogenesis induced by urethane. MethodsThe mouse lung carcinoma was induced by intraperitioneal urethane 1 000 mg /
kg. The animals were treated with lycopene for 18 weeks to investigate the rate of tumor formation.The activities of SOD, CAT, GST and the contents of MDA, IL 2 and TNF α in
serum and in lung homogenate were tested.ResultsLycopene could
increase the activities of SOD and CAT, increase the content of IL 2 and TNF α, prevent GST and MDA from increasing, reduce the rate of carcinogenesis.Conclusion Lycopene can
prevent lung carcinogenesis induced by urethane, which might be related to its antioxidantion and immune-regulation.
Key words: Lycopene; Urethane; Lung Carcinogenesis
Lycopene (lycopene, Ly) for the open-chain unsaturated
carotenoid, is widely distributed in a variety of plants, which in the tomato fruits with higher levels of maturity, each 100 g of up to 3 ~ 14 mg . Modern medical studies have proven that lycopene has anti-oxidation, scavenging free
radicals, promote cell inter-connection and transmission,
anti-aging and other physiological functions . Studies have
shown that certain human cancer incidence and dietary levels of lycopene there is a close relationship. Therefore, it was predicted that lycopene may be a very effective anti-cancer
dietary components . To verify whether lycopene exact anti-
cancer effect, we observed the lycopene on urethane-induced
lung effects in mice.
1.1 Reagent urethane (Shanghai Chemical Reagent Factory product, batch number 030,412). SOD, CAT, GST, and MDA Kit (Nanjing Jiancheng Biotechnology Institute). TNF α and IL
2 ELISA detection kit (Sino-American Biotechnology
Company). Cellulase, Wuxi plant biological agents. Other reagents were analytical pure.
1.2 Animals Kunming mice, male, weighing 20 ~ 23 g (Experimental Center, Henan Medical animals Qualified
Certificate No.: medical action Zi No. 410,115); mouse lung tumor strain, Henan University, College of Pharmacy Room of cells.
1.3 Instrument rotary evaporator and automatic water stills, Rong Biochemical Instrument Factory Shanghai Yali;
ultrasonic generator, Kunshan Ultrasonic Instrument Co., Ltd.; 721 spectrophotometer, Shanghai third analytical instrument factory; 550 microplate reader, Bio-RAD.
1.4 Preparation of fresh tomatoes subjects amount of drugs, organized compactors beating, per 1 000 g of fruit by
adding 800 ml of ethanol and 250 g ammonium sulfate, oscillation 5 min, placed 10 min after the phase separation, to remove flesh away from the upper and lower phase, per 1 000 g pulp by adding 5 g of cellulose enzyme mixture, 45 ? role
of the 30 min, per 1 000 g pulp ethanol 1 000 ml, 40W ultrasound 30 min, centrifuged to remove pulp, rotating evaporation of ethanol, vacuum drying to be tomato Red Pigment. HPLC measured pulp content was 14.2 mg/100 g, purity
greater than 99%. When used with 0.5% CMC Na solution,
dissolved, add a small amount of Tween-made suspension, for
2.1 tumor model to take inoculation of mice 8 d grow well armpit tumor block, using saline ground into a cell
homogenate, in order to # 6 needles absorb cell sap, adjusting cell number of 5 × 106/ml, according to 0.2 ml / inoculation only the right armpit subcutaneously in mice, produced tumor model .
2.2 The research program will be 70 female Kunming mice
were divided into three groups: ? the model group 30: After
modeling, conventional feeding; ? lycopene Group 30: After
modeling, ig lycopene 10 mg / kg; ? Normal the control group
10: Do not build models, parallel feeding. Experimental day 1,
model control group and lycopene, the mice were ip urethane 1000 mg / kg 1 times, the first two weeks are beginning to give lycopene, 1 / d, 5 times / week. The first 18 weeks to mice with abdominal aortic bled, the serum -80 ?
preservation; the same time, mice were the lungs, first
observe whether the tumor is generated (if any, records the number of tumors, and tumor length measured with vernier caliper diameter and width of a path b , according to tumor = 0.4 × a × b calculate the size), followed by the lungs in
liquid nitrogen pulverization, plus two re-distilled water
homogenate obtained after centrifugation supernatant 80 ?
preservation, is used to detect SOD, CAT, GST activity and MDA , IL 2 and TNF α content.
2.3 Determination of SOD, CAT, GST activity and MDA, IL
2 and TNF α levels according kit instructions,
including SOD, CAT, GST, and MDA by colorimetry; IL 2 and
TNF α with ELISA was determined, all colorimetric ELISA
plates were 96 by the 550 microplate reader to complete.
2.4 The statistical results of this paper are ? s said
that the difference between the groups using single-factor
analysis of variance, completed by SPSS11.0. P <0.05, indicated a significant difference; P <0.01, indicated a very significant difference. Reposted elsewhere in the paper for free download http://
3.1 Effect of lycopene on the formation of urethane induced lung SOD, CAT activity and IL 2 and TNF α
content in comparison with the normal control group, model
control group, serum and lung tissue SOD, CAT activity has a very a significant difference (P <0.01), IL 2 and TNF α
levels were significantly different (P <0.05), shows the model group serum and lung tissue SOD, CAT activity and IL 2 and
TNF α were significantly reduced, lycopene group and normal control group, no significant difference (P> 0.05); with the model control group, serum levels of lycopene and lung tissue SOD, CAT activity were significantly different (P <0.05), IL -2 and TNF α levels are very significant
differences (P <0.01), shows lycopene can increase serum and lung tissue SOD, CAT activity, so that IL 2 and TNF α
levels rise. The results in Table 1.
Table 1 Effect of lycopene on the formation of urethane induced lung cancer in serum and lung SOD, CAT activity and IL
2 and TNF α content in (omitted)
With the model control group, * P <0.05, ** P <0.01; with the normal control group, # P <0.05, # # P <0.01
3.2 Effect of lycopene on urethane-induced lung cancer in
mice during the formation of GST activity and MDA content in comparison with the normal control group, model control group, serum and lung tissues of GST activity in a very significant
difference (P <0.01), MDA content in significant difference (P <0.05), shows the model group serum and lung tissues of GST activity and MDA levels were significantly higher, lycopene and normal control group, no significant difference; with the
model control group, serum levels of lycopene and GST activity in lung tissue were significantly different (P <0.05), MDA
content in a very significant difference (P <0.01), shows lycopene on serum and lung tissues of GST activity and MDA levels were reduced role (see Table 2).
Table 2 Effect of lycopene on the formation of urethane induced lung cancer in serum and lung GST activity and MDA content in (omitted)
With the model control group, * P <0.05, ** P <0.01; with the normal control group, # P <0.05 # # P <0.01
3.3 Effect of lycopene on the formation of urethane induced lung cancer rate of the control group compared with the model, lycopene group decreased tumor incidence, tumor number were significantly different (P <0.05), tumor size,
there are very significant differences in (P <0.01), shows lycopene can reduce the incidence of cancer, inhibiting tumor growth (see Table 3).
Table 3 Effect of lycopene on the formation of urethane induced lung effects (abbreviated)
With the model control group, * P <0.05, ** P <0.01
Lycopene is a bright red color of natural carotenoids, the antioxidant vitamin E, 100 times that of β-carotene 2
times, has therefore become a hot health food research .
Urethane-induced lung cancer, effects on mice, which is characterized as not affect the animals, diet and body weight, no dose accumulation effect . We use urethane-induced lung
cancer model in mice to study lycopene's effect on the tumor and found that lycopene can reduce the incidence of cancer, so that fewer tumors, smaller in size, indicating that tomato red is known as the exact tumor prevention and inhibit tumor growth effect. Lycopene immune regulation, can increase the spontaneous breast cancer in mice T-lymphocyte subsets CD 4,
raising CD 4/CD 8 ratio, so that abnormal T-lymphocyte
mutation, returned to normal , this may be the block tumor, inhibiting tumor growth is one important reason. In this study, elevated serum lycopene and lung IL-2 and TNF-α
levels, lower GST activity and MDA content, combined with Lycopene-induced apoptosis of tumor cells of some reports , we speculate tomato Red pigment from anti-cancer effect may be
adjustment of the body's own micro-environment is not
conducive to the development of induced tumors.
Of free radicals induced cancer, tumor promotion and carcinogenesis has been recognized, its formation and the elimination of imbalances with tumor occurrence and development of close ties . SOD, CAT, GST part of the body
to eliminate free radicals and lipid peroxidation enzymes, they are with other enzymes and antioxidants the body to form a free radical defense system to remove excess free radicals . The experimental results showed that lycopene in the
urethane-induced lung cancer in mice against the formation of SOD, CAT activity has picked up the role, and can stop with the corresponding GST activity in cancerous and the MDA content increased, once again validates our lycopene by regulating the body's own micro-environment and anti-cancer
effect of speculation.
China is the largest producer of tomato, lycopene its own unique health functions that people gradually recognized. There is a growing emphasis in the current green, healthy circumstances, using anti-oxidation, anti-aging as a guide,
its position in the anti-cancer, anti-cancer natural health
food products to develop, will have broad market prospects.
 Hsu Wen-Ling, Li Yan, Wang Xuexia, et al. Lycopene
extraction and physiological function of [J]. Agro-processing
Journal, 2006,70 (7): 4.
 Chia-Le, Fu-sheng, LUO Xue-Ya, et al. Lycopene
biological function and Preparation Methods [J]. Food and Drugs, 2006,8 (8): 15.
 Wei-Hua Wang, Han Zhan-jiang, functional properties
of lycopene and its application research [J]. Natural products research and development, 2006,18 (1): 139.
 Shuzhen Bo, Cao Hai-ping. Thalidomide, paclitaxel
inhibition of Lewis lung cancer mice [J]. Practical Journal of
Cancer, 2003,18 (5): 379.
 Yang-Hui, Chang-dong, PAN Hong-zhi, et al. Lycopene
of mouse anti-fatigue and anti-oxidation [J]. Chinese Journal
of Clinical Nutrition, 2006,14 (3): 141.
 Terry W. Moodya, Julius Leytona, Farah Ziaa et al.
Thymosina1 is chemopreventive for lung adenoma formation in A / J mice [J]. Cancer Letters, 2000,155 (2): 121.
 Xi-Hua Pan, Zhong Jian, Xiao Ping, et al. Lycopene, modulate immune function and mechanism [J]. Shanghai Journal of Preventive Medicine, 2006,18 (6): 261.
 Zhang Weiguo, Guo-Jian Xie, Xian-Jun Liu, et al.
Lycopene on gastric cancer cells SGC 7901 Growth Inhibition
of [J]. Chinese Pharmacology Bulletin, 2005,21 (3): 292.
 Jayne Sierens, John A. Hartley, Maeli J. Campbell et
al. Effect of phytoestrogen and antioxidant supplementation on oxidative DNA damage assessed using the comet assay [J]. Mutation Research, 2001, 485 (2): 169.
 Estrada Garc? 'A. L, Carrera-Rotllan. J, Puig
Parellada. P. Effects of oxidative stress and antioxidant treatments on eicosanoid synthesis and lipid peroxidation in long term human umbilical vein endothelial cells culture [J]. Prostaglandins