EB virus latent membrane protein (LMP1) and expression of survivin in nasopharyngeal carcinoma
【Abstract】 Objective To investigate the nasopharyngeal squamous cell carcinoma of EB virus latent membrane protein LMP1, and survivin expression and prognosis. Methods The
immunohistochemical SP method, 83 cases of nasopharyngeal squamous cell carcinoma of the organizations LMP1 and survivin tags, analyzed the clinical stage, radiation therapy within three years after the results of relationship. The results of
LMP1 expression and clinical stage (P> 0.05), but relapse within three years after radiotherapy (P <0.05). survivin and clinical stage, relapse within three years after radiotherapy (P <0.05). LMP1 and survivin are associated (P <0.05).
Conclusion LMP1 and survivin in nasopharyngeal carcinoma has important prognostic value, can be used for clinical treatment of the reference index.
Key words nasopharyngeal carcinoma LMP1; survivin immunohistochemistry
Expressions of Epstein Barr Virus Latent Membrane Protein
1 (LMP1) and survivin in Nasopharyngeal Carcinoma and their Clinical Significance
Key words: Nasopharyngeal; Carcinoma; LMP1; survivin; Immunohistochemical method
NPC (Nasopharyngeal carcinoma, NPC) is a common malignant tumor in China, particularly Guangdong and Guangxi areas of high-fat, with the gradual in-depth on the NPC study, EBV
infection and is closely related to people's NPC has been recognized. survivin is an inhibitor of apoptosis, studies
have shown that the degree of survivin expression in stage of disease progress and the extent of malignant tumor tissue is consistent. We used immunohistochemistry to detect NPC in
poorly differentiated squamous cell carcinoma of the most
common pathological tissues and survivin expression of LMP1, combined with clinical stage, three years after radiotherapy for recurrence of such information as to whether the study to explore both in the NPC the role of the relationship and its significance.
1 Materials and methods
83 cases of nasopharyngeal carcinoma specimens were from our hospital in January 1998 ~ January 2002 close examination of the paraffin-embedded tissues.
NPC clinical stages of nasopharyngeal carcinoma according to the National Conference, Fuzhou in 1992, recommended standards , the 28 patients with stage ? ~ ?, ? period
of 26 cases, ? period 29 patients were divided into three groups; by radiotherapy would be cases of relapse within three
years whether the were divided into 48 cases without recurrence group and recurrence of group two sets of 35 cases.
1.3 Immunohistochemical detection method
Using peroxidase labeled streptavidin staining S P
method. Using an antibody were purchased in the United States Santa Cruz company's products, using the work of the concentration of antibodies: mouse anti-human monoclonal
antibody LMP1 1:80, rabbit polyclonal antibody anti-human
survivin 1:100, SP kit purchased from Beijing Zhongshan
Company, the entire process carried out according to kit instructions. With phosphate buffer (phosphatic buffered saline, PBS) instead of a resistance as negative control.
LMP1 in cancer cells cytoplasm, membrane yellow-brown
granules, survivin in cancer cell nuclei and cytoplasm yellow-
brown granules, the number of positive cells "of 5% positive mark.
1.5 Statistical analysis
Using SPSS11.5 software, χ2 test. PEMS3.0 software,
correlation analysis. P <0.05 significant differences.
2.1LMP1 and survivin in nasopharyngeal carcinoma in different clinical stages of expression groups
(1) LMP1 in ? ~ ? stage cases, 28 cases of negative
expression shown in Figure 1, expression shown in Figure 2, the positive rate is higher than in stage ? case group, below
the case group stage ?. However, LMP1 in different stages of the positive rates showed no significant difference. (2)
survivin in ? ~ ? stage cases, 28 cases of negative
expression shown in Figure 3, positive expression shown in Figure 4, the positive rate of less than the case group stage ? and ? case group, ? ~ ? Phase The positive rate of stage ? Group, ? stage group was statistically significant positive rates, ? period, ? stage group showed no
significant difference in positive rates in Table 1. Table 1 LMP1, survivin expression and clinical stages of NPC poorly differentiated squamous cell carcinoma of the relationship
between the Note: LMP1 in the group stage ? ~ ? and stage ?
positive rate of positive rate of a comparison: χ2 = 0.0593,
P = 0.08075; ? ~ ? Phase Group stage ? positive rate and
positive rate of a comparison: χ2 = 0.0128, P = 0.9101; ?
period The positive rate of stage ? a comparison group: χ2 =
0.1271, P = 0.7215. survivin in the group stage ? ~ ? and
stage ? positive rate of positive rate of a comparison: χ2 =
6.4803, P = 0.0109; ? ~ ? stage group the positive rate and positive rate of stage ? a comparison: χ2 = 7.9875, P =
0.0047; ? period The positive rate of stage ? a comparison
group: χ2 = 0.0458, P = 0.83052.2LMP1 three years after radiotherapy without recurrence of the case group than in the positive rate of recurrent cases within three years after
radiotherapy group, in comparison with there are statistically significant. survivin in the three years after radiotherapy without recurrence of the cases positive rate of less than three years after radiotherapy for recurrence of the disease
group, in comparison with statistical significance, Table 2. Table 2LMP1, survivin expression and recurrence after radiotherapy for three years whether the relationship between
the Note: LMP1 three years after the radiotherapy group and no recurrence after radiotherapy positive rate and positive rate of recurrence within three years of comparison: χ2 = 5.3989,
P = 0.0201. survivin in the radiotherapy group and no recurrence three years after the positive rate of recurrence within three years after the radiotherapy group comparison of positive rates: χ2 = 7.4532, P = 0.0063. 2.3LMP1 with
survivin expression in nasopharyngeal carcinoma in the relationship between the Table 3 the two between factors statistically significant correlation. Expression and survivin
expression in Table 3LMP1 Note: LMP1 and Survivin correlation between the two factors: r = 0.4960, P = 0.0000