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Drinking game of the brain stem lesion cerebral ischemia-reperfusion injury in rats_157

By Darlene Wood,2014-10-30 15:34
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Drinking game of the brain stem lesion cerebral ischemia-reperfusion injury in rats_157

Drinking game of the brain stem lesion cerebral ischemia-

    reperfusion injury in rats

     Abstract Objective To observe the brain stem to drink on focal cerebral ischemia-reperfusion brain injury in rats and to further explore its mechanism. Methods The middle cerebral artery embolization in rats produced focal cerebral ischemia-

    reperfusion injury model. The use of immunohistochemistry, respectively, TTC staining and Zea Longa 5-point scale scoring

    system to observe the brain stem in brain tissue of treated

    drinking Bcl 2 protein expression, infarct volume and neurological changes in behavior. Results Compared with model group, treatment group significantly reduced symptoms of neurological impairment (P <0.05); infarct volume reduced by

    about 40%; in each time point Bcl 2 expression in both the

    high and the model group (P <0.05). Conclusion brain stem drinking by Prostisol, Huoxue network of comprehensive control measures can not only increase apoptosis inhibitory gene Bcl

     2 expression, reduced infarct volume, but also can improve the symptoms of nerve injury, reducing structural damage brain tissue, lack of brain Blood-reperfusion injury caused

    significant protection.

     Key words Brain stem drink Bcl 2 protein in ischemia-

    reperfusion injury

     Experimental Study of the Protective Effects of Naogengyin on Cerebral Ischemia-reperfusion Injury in Rats

     Abstract: ObjectiveTo measure the protective effects of Naogengyin on cerebral ischemia reperfusion injury in rat

    and its mechanisms.MethodsThe model of focal reperfusion injury after cerebral ischemia in rats was made by middle cerebral artery occlusion. The effect of Naogengyin on

expression of Bcl 2 proin, cerebral infarct volume and

    behavioral change in rats brain tissue were observed by

    immunohistochemical method, TTC staining and Zea Longa method respectively.ResultsCompared with the control group, neurological grades in Naogengyin group were lower (P <0.05). The volume of cerebral infarction decreased 40% in treated

    groups. Bcl-2 expression of the treated groups increased more than that of the control groups in each hour point. There was great difference between the control groups and treated groups in expression of Bcl 2 protein (P <0.05 ).

    ConclusionNaogengyin has great cerebral protective effects

    against ischemia-reperfusion injury by increasing restrain mechanisms of the apoptosis of neuronal cell and expression of Bcl 2 protein, reducing the volume of cerebral infarction and damage to brain tissue.

     Key words: Naogengyin; Bcl 2protein; Ischemia

    reperfusion

     Acute cerebral ischemia - reperfusion (IR) injury can

    cause severe neurological dysfunction, and even life-

    threatening, to find effective treatments and measures to promote functional recovery after stroke central nervous

    system has been the focus of the study of related disciplines . In recent years, Chinese medicine on the brain protective effect of more and more attention. Drink is a Professor Wang Youqi brain stem stroke prevention and treatment of clinical experience, common side, in the years of clinical use to obtain good effect. In this study, inhibition of apoptosis and reduced brain infarct volume point of view in the brain stem to drink in rats.

     1 Materials and methods

     1.1 Materials

     42 healthy Wistar rats, weighing 250 ~ 300 g. The brain stem to drink from Astragalus, habitat, 37, the Health Puhuang etc., these drugs are filtered decoction condensed into 100 ml (containing crude drug 1.2 g / ml). Bcl 2 monoclonal

    antibody, DAB chromogenic kit, HIGH SAB kit were purchased

    from Wuhan Boster Company.

     1.2 Methods

     1.2.1 Methods of modeling documents using the light of improved Koizumis method [1] Preparation of MCAO animal model. The external carotid artery ligation in rats, since the common carotid artery bifurcation to the carotid artery into plug-

    line (18.5 ? 0.5) mm, block the middle cerebral artery blood flow, resulting in focal cerebral ischemia. After the tail of the plug wire placed through the skin, ischemia and 2 h after

    the bolt of light pulling lines caused by blood reperfusion. Survival in rats observed after 6,12,24 h reperfusion in rats changes in behavior.

     Zea Longa behavioral ratings refer to the five-point

    scale score criteria: ? 0 points: normal, no symptoms of

    nerve injury; ? 1 pm: contralateral forepaw flexion or full flexion part; ? 2 points: the hemiplegic side turning in circles; ? 3 points: the hemiplegic side of dumping; ? 4

    points: You can not spontaneously walk, loss of consciousness.

    Sham-operated group, in addition to non-insertion lines, the

    remaining steps ibid.

     1.2.2 Grouping and administration will be making a successful model rats were randomly divided into sham operation group, model group and treatment group. In addition

    to sham-operated group, the other in each group is further divided into two groups after ischemia-reperfusion 6,12,24 h 3

    teams, a total of seven groups, the ultimate success of surgery combined 35 experiments in each group 5. Sham-

    operation group, model group and treatment group 1 week before surgery, respectively, by 1 ml/100 g / times, 2 times / d given continuously fed to drink salt water and the brain stem. Treatment group, 3 h before surgery and postoperative 3,6,12 h respectively of the gavage one time.

     1.2.3 Brain Bcl 2 protein in rats after ischemia-

    reperfusion, respectively, at different time points, excessive anesthesia, open chest, exposing the heart, with 4 ? heparin

    saline (2 × 105 u / L) 150 ml quick wash, followed