Detection of drug resistance and gene type
Author: Zhengya Fen Zhou Wei Zi Jing Cheng Xiao-
【Abstract】 Objective To understand the neonatal nosocomial infection producing extended spectrum β lactamases (ESBLs)
producing Klebsiella pneumoniae isolates resistant conditions and β lactamase gene encoding TEM, SHV, OXA, PER, GES, VEB, CTX-type situation there. Method in our hospital in July 2004 infection in neonatal wards in the 14 strains of Klebsiella pneumoniae with automatic microbial identification and antibiotic susceptibility analysis of matching cards for susceptibility testing and ESBLs detection and polymerase chain reaction and sequence analysis method Analysis of strain in ultra-broad-spectrum β lactamase. The results of 14
ESBLs-producing Klebsiella pneumoniae susceptibility results are consistent, ampicillin, ceftriaxone, cefazolin, ceftazidime were resistant to amoxicillin / clavulanic acid, piperacillin / tazobactam resistance is also , for imipenem.
Were detected blaCTX M and blaTEM genes were not detected blaSHV, blaOXA, blaPER, blaGES and blaVEB genes. Right blaTEM and blaCTX M gene amplification products were sequenced by BLAST program analysis gene TEM 1 and CTX M 3-type.
Conclusion hospital infection in the popular ESBLs Klebsiella pneumoniae resistant to third generation cephalosporins and resistance to enzyme inhibitors, gene type TEM 1 and CTX
M 3. ESBLs belonging to the high risk newborns, neonatal diseases property extended spectrum β lactamase bacterial
infections to be caused by all sides.
Key words Newborn ultra-extended-spectrum β-lactamase gene
types of Klebsiella pneumoniae
Investigation of the antibiotics resistance and resistant
ABSTRACT Objective To investigate the antibiotics resistance of ESBLs producing Klebsiella pneumoniae (KPN) which were isolated from newborns and the genotype existence of TEM, SHV, OXA, PER, GES, VEB and CTX. Methods The antimicrobial susceptibility and ESBLs detection of fourteen
KPN isolates from newborns in the Materity and Children Health Hospital of Changzhou, in July, 2004 were performed. The genotype of ESBLs from KPN were identified by polymerase chain reaction (PCR). Results Total of 14 KPN producing ESBLs were
resistant to ampicillin, ceftriaxone, cefazolin , ceftazidime, amoxicillin / clavulanic acid and piperacillin / tazobactam, while sensitive to imipenem. All 14 KPN carried blaCTX M
gene and blaTEM gene, whereas blaSHV, blaOXA, blaPER, blaGES
and blaVEB genes were not detected. blaCTX M gene and
blaTEM gene were identified as blaTEM 1 and blaCTX M
3. Conclusion The KPN isolated from newborns were resistant to the third generation cephalosporins and enzyme inhibitors, and the genotype were TEM 1 and CTX M 3. As newborns are
a high risk group, it should be pay more attention to keep them from the ESBLs producing KPN epidemic outbreak.
KEY WORDS Newborns; ESBLs; Klebsiella pneumoniae; Genotype
Nosocomial infection in intensive care unit producing
extended spectrum β lactamase-resistant strains of
bacteria isolated and drug resistance genes have been repeated reports of episodes of domestic [1 ~ 4]. Neonatal nosocomial infection producing extended spectrum β lactamase isolated
bacteria resistance and resistance gene studies have reported relatively few. In our hospital in 2004 have occurred in a neonatal ward of Klebsiella pneumonia