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Deliciosa stem Pharmacognosy Identification_129

By Sam Jordan,2014-10-30 14:52
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Deliciosa stem Pharmacognosy Identification_129

Deliciosa stem Pharmacognosy Identification

     Abstract Objective To pharmacognosy research, kiwifruit stem identification and exploitation of providing a scientific basis. Method of powder production method, paraffin section method, UV absorption spectroscopy, thin layer chromatography. Results traits characteristics: in the pericycle fibers and stone cells are co-exist with more phloem-secreting cells,

    milk tubes and sieve scattered; powder can be seen more wood fiber and bast fibers. Physical and chemical identification features: ethanol extract at the maximum absorption at 212.5 nm, chloroform extract at 246.0,412.5 nm at maximum absorption. Conclusion The results can be used as basis for identification of stem Actinidia deliciosa.

     Key words Actinidia deliciosa microscopic identification UV absorption spectra of TLC

     Pharmacognostic Studies on the Stem of Actinidia deliciosa CFLiang et AR Ferguson

     Abstract: ObjectiveTo provide a scientific basis for identification and utilization of the stem of Actinidia deliciosa CFLiang et AR Ferguson. MethodsPowder slide paraffin sectionslide, UV, TLC were used.ResultsMicroscopic characteristics: in stem transverse, fibers and stone cells were found in pericycle. Secretory cells, milk vessel and

    sieve tube were found in phloem.There were many phloem fibers and xylem fibers in powder. UV: Ethanolic extract of stem had a absorption peak at 212.5 nm, chloroformic extract of stem had two absorption peaks at 246.0,412.5 nm. ConclusionThe

    results can be taken as the reference for identification of the stem.

     Key words: Actinidia deliciosa CFLiang et ARFerguson; Microscopical characteristics; UV; TLC

     Actinidia deliciosa Actinidia deliciosa CFLiang et ARFerguson from Actinidia Actinidia [1]. People with its

    roots, root bark to treat hepatitis, edema, rheumatoid arthritis, bruises, dysentery, filariasis, lymph nodes tuberculosis, carbuncle swollen boils drugs, chyluria, vaginal discharge, stomach cancer, breast cancer; with Qingrejiedu,

    swelling blood circulation, expelling wind dampness efficacy [2,3]. Anti-tumor effects in vitro study found that kiwifruit roots, stems extracts inhibit the leukemia cell line L1210 and P388D1, cervical cancer cell line Hela, human gastric cancer

    cell line SGC7901 and other tumor cell lines proliferation, and may result in some cells apoptosis and death [4]. I have Actinidia deliciosa roots, leaves of characteristics of raw pharmaceutical research. This stems for their micro-

    identification, identification and other aspects of physics and chemistry pharmacognosy research for future identification and development of the use of herbs to provide some scientific basis.

     1 Equipment

     1.1 Materials

     Actinidia deliciosa 2002 04 collected from Guilin,

    Guangxi region, Guangxi Institute of Botany, Chinese Academy of Sciences of plants through the classified room identified as Actinidia Actinidia deliciosa Actinida deliciosa CFLiang et AR Ferguson of the stem, specimens stored in the Guangxi

    College of Pharmaceutical Analysis Teaching and Research.

     1.2 Instrument and reagents

     UV 160A spectrophotometer (Shimadzu Corporation), ZF-

    I-type three-ultraviolet analyzer (Shanghai Gu Village, Electro-Optical Instrument Factory), RM2145 microtome (Germany

    LEICA Inc.), YG 280KX spread sheet baking sheet machine ( Hubei Sunshine Medical Equipment Co., Ltd.), MPS60 and Qwin biological microscope image analysis system (LEICA, Germany company), the remaining drugs and reagents were analytical

    pure.

     2 Methods and Results

     2.1 medicinal properties

     Elongated cylindrical stem diameter of 1 ~ 4 cm, the surface of brown or grayish brown, a bit like lenticels and longitudinal wrinkles, showing that leaves marks. Quality hard aspect gray, hip loose into the hollow. Gas micro-and taste

    slightly astringent. Reposted elsewhere in the paper for free download http://

     2.2 Microscopic identification

     Stem cross-section 2.2.1

     Epidermal cells 1, class round or oblong; outside the

    non-glandular hairs; skin beneath the thick horn of 3 ~ 4. Cortex is relatively narrow, round-cell type. In the pericycle

    fibers and stone cells are co-exist. Phloem narrow, there are more secretory cells, breast milk tubes and sieve scattered. Cambium more obvious, from 3 ~ 4 cells, arranged in close. Narrow xylem, fibers less; ray 1 cells, the radial arrangement, a single catheter scattered. Hip broad, from large-scale wood-based micro-thin-walled cells; a single

    secretory cells scattered. Figure 1 ~ 3.

     2.2.2 Stem Powder

     For the yellow-white powder. Mucous cells, there are two, one for the long oval-shaped, long 440.0 ~ 650.0 μm, width

    99.5 ~ 170.0 μm, another way for the class round, diameter of 55.0 ~ 70.6 μm. Scattered needles of calcium oxalate crystal

    thick, long 48.6 ~ 50.6 μm. Irregular-shaped stone cell,

    there are rectangular, triangular, round-like and so on,

    diameter of 33.7 ~ 87.1 μm, walls thin, circular pits. Wood fiber long spindle-shaped, slightly pointed at both ends,

    circular pits, wall thick, diameter 20.0 ~ 30.0 μm. Bast

    fiber long spindle-shaped, one end slightly blunt one end slightly pointed, thin wall, there is one side was slightly interdigitating dendritic diameter of 25.0 ~ 35.0 μm.

    Epidermal cell type rectangular-shaped wall thickening of

    Pente. Non-glandular hairs mostly curved, single-cell non-

glandular hairs, long 480.0 ~ 580.0 μm. Catheters are more

    prevalent thread, reticulate, and bordered pits. Milk tube containing yellow milk. Starch granules are more single-grain

    multi-cylindrical spherical, umbilical little bit of shape, layer pattern is not obvious, complex particles from 2 to 3 pm particles formed. Hole pattern tracheid diameter of 5.5 ~ 9.5 μm. Brown block irregular in shape, diameter and 16.8 ~ 18.0 μm. Figure 4.

     2.3 UV absorption spectra obtained crude medicinal powder (over 40 mesh sieve) 4 g, respectively, ethanol, chloroform 60 ml, cold soak 48 h, filtration, the filtrate diluted due to solvent blank, was 200 ~ 500 nm within the scanning, mapping UV absorption curve, the results of ethanol extract at 212.5 nm at the maximum absorption, chloroform extract at 246.0,412.5 nm at maximum absorption.

     2.4 TLC identification method according to the stem of

    the filtrate obtained. Point in the CMC-Na (0.7%) - silica gel

    G plate in order to chloroform - methanol (20:1) as the agent

    to start the show from the 14 cm, Hui dry solvents, ultraviolet analyzer (365 nm) observed under the fluorescent spots. The results shown in Figure 5-a; with 10%

    phosphomolybdic acid test solution color, 105 ? heating 5

    min, the remarkable blue spots on a yellow background, the results shown in Figure 5-b.

     3 Discussion

     Actinidia deliciosa stem cross-section of pericycle

    fibers and stone cells are co-exist; phloem narrow, there are

    more secretory cells, breast milk tubes and sieve scattered. Powder, irregular-shaped stone cells can be seen, there are rectangular, triangular, round-like and so on, round pits;

    wood fiber long spindle-shaped, both ends slightly pointed,

    round pits; bast fiber long spindle-shaped, one end slightly

    Blunt, one end slightly pointed, thin wall, there was micro-

    sudden-like side. Identification of these features can serve as reference.

     Actinidia deliciosa ethanol extract of stem in the Ultraviolet Region Department has absorbed 212.5 nm, chloroform extract in the Ultraviolet Region 246.0,412.5 nm Department has to absorb, but the characteristic peaks of the

specificity is not strong, can be used as identification aids.

     References

     [1] Flora of China Editorial Committee of Chinese Academy of Sciences. Flora of China, 49 volumes, the first two volumes [M]. Beijing: Science Press, 1984:261.

     [2] the national assembly to prepare herbal medicine

    group. Nationwide compilation of Chinese herbal medicine, on the book [M]. Beijing: People's Health Press, 1975:795.

     [3] Jiangsu New Medical College. Dictionary of Chinese medicine, the next book [M]. Shanghai: Shanghai Science and

    Technology Press, 1977:2210.

     [4] Zhongzhen Guo, Zhang Fengfen, Han-Shen Zhen, et al.

    Deliciosa root extract anti-tumor effects of the experimental

    study [J]. Traditional Chinese Medicine Journal, 2004,22 (9): 1705. Reposted elsewhere in the paper for free download http : / /

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