Coptis accumulation of active ingredients_715

By Rodney Wallace,2014-10-30 14:15
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Coptis accumulation of active ingredients_715

Coptis accumulation of active ingredients

     Author: Ma Kai-sum, Shi-Jiang Chen De-Li Zhang


     Abstract Objective To study the Coptis (flavored with) the dynamics of accumulation of the active ingredient, in

    order to determine the optimal harvest time provide the basis Coptis. Fixed-point method on a regular basis collected 2 to 5 year-old Coptis samples, analysis of berberine hydrochloride Coptis rhizome and total alkaloid content, respectively, by high performance liquid chromatography (HPLC) method and UV method. Results Coptis rhizomes of dynamic accumulation of the active ingredients have certain laws, berberine content in April each year (flowering and fruiting period) is almost the whole year were the lowest, 4 ~ 5 years old to 10 November

    Coptis Coptis rhizomes of berberine content in at their annual maximum value, while a 5-year-Coptis of berberine content was

    the highest; the total alkaloid content of an upward trend in each of the 10 levels at their annual maximum in November,

    while a 5-year-total alkaloid content of berberine maximum. Conclusion According to the accumulation of Coptis dynamics of the active ingredient, berberine best harvesting period of 10 to 11 months of 5-year-Coptis rhizomes.

     Key words berberine dynamic accumulation of active ingredients

     Abstract: ObjectiveTo study the dynamic accumulation regulation of the effective components of Coptis chinensis, so to provide the experimental data of optimal harvest time of Coptis chinensis. MethodsThe samples of two to five years were gathered from the same glebe and time. Berberine and the total alkaloids were analyzed by HPLC and UV methods. ResultsThe

    dynamic accumulation of effective components in the rootstalk of the Coptis chinensis had regularity in the certain extend. The contents of berberine in samples were almost the lowest in April in every year, which were mostly the highest in October and November from the samples grown 4 ~ 5 years, but the content of the component was mostly the highest in 5-year-old

    Coptis chinensis in the series samples. The content of the total alkaloids increased year by year in the rootstalk of the Coptis chinensis, which was mostly the highest in October and November in every year, but the content of the component was

    mostly the highest in 5-year-old Coptis chinensis in the

    samples. ConclusionAccording to the accumulation pattern of the effective components in the Coptis chinensis, the optimal harvest time is from September to November.

     Key words: Coptis chinensis; Effective components; Dynamic accumulation

     Coptis Coptis chinensis Franch cultivation history in China planted earlier and export of Chinese herbal medicines commonly used medicinal herbs, there are more than 600 years of cultivation history [1], produced in Chongqing, the main pillars of its national production output accounts for 40% ~ 60%, quality. To this end, Shizhu have "Coptis town" reputation. Coptis rhizomes of berberine and total alkaloid berberine is the main active ingredients, is to examine the

    quality of key indicators Coptis this end, we have studied the Coptis rhizomes of berberine and total alkaloid content of the law of accumulation, in order to determine the optimum harvest period of Coptis to develop standard operating procedures

    provide a scientific basis Coptis.

     1 Materials and methods

     2002-04 onwards, regular, fixed collection Shizhu yellow water Coptis farm house Cong Tan (elevation 1 300 m) the same plot, the same growth and age, the size of basically the same

    2 ~ 5 year-old plant samples Coptis rhizome dry thermostat back.

     Coptis rhizome 1.1 Determination of berberine

    hydrochloride in

     1.1.1 Instrument and test drugs instruments: high-

    performance liquid chromatography: waters the company's

    Alliance2690 pump, 996 diode array detector; Millennium32 chromatography workstation, ultrasonic cleaner (KQ-50B type);

    four-hole separate constant temperature water bath pot .

     Test drugs: Berberine hydrochloride reference substance

    by the Chinese pharmaceutical and biological products to offer batch 0713-200107 (for HPLC use) chromatography pure

    acetonitrile, methanol and other reagents were of analytical grade, water-deionized water .

     1.1.2 Column Methods: Dima's Dimonsil C18 (4.6 mm × 250

    mm, 5 μm); pure mobile phase, acetonitrile -0.1 mol potassium

    dihydrogen phosphate (35:65) detection wavelength of 345 nm, column Greenhouse temperature, flow rate 0.7 ml * min-1.

     1.1.3 linear relationship between the study said

    precision reference substances 5.45 mg of berberine hydrochloride to take home 25 ml Liang Ping, add the amount of dissolved methanol, together with methanol was diluted to scale, blending, attainable standards of reserve liquid. Precision drawing this standard reserve liquid

    0.2,0.5,1.0,2.0,4.0,5.0 ml, set 10 ml Liang Ping, add methanol

    was diluted to scale, get six different concentrations of contrast solution, respectively, precise drawing 20 μl, into

    the liquid chromatography in the determination of record peak area, and to the peak area (Y) of the sample volume (X) regression, have standard curve equation:

     Y =- 421 5 1 660 625X (r = 0.999 8)

     To peak area (Y) - sample volume (X) is graphed, it was a straight line, experimental results show that: The 0.087 2 ~ 2.18 μg range berberine peak area and sample volume has a

good linear relationship between . The results in Table 1.

     Linear relationship between the experimental results in Table 1 (omitted)

     1.1.4 draw on the control precision of experimental precision solution of 20 μl (concentration of 0.087 2 mg /

    ml) repeated injection five times, the determination of berberine peak area recorded the results in Table 2.

     Precision of experimental results in Table 2 (omitted)

     Experiments show that good accuracy.

     1.1.5 stability test to take samples for test solution, 0,4,8,12,16 h, respectively, were injection 20 μl, the

    determination of berberine hydrochloride in the peak area recorded the results in Table 3.

     Table 3 Stability test results (omitted)