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Complex Armadillidium decoction Experimental Study of analgesic effect of_244

By Josephine Warren,2014-10-30 13:48
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Complex Armadillidium decoction Experimental Study of analgesic effect of_244

    Complex Armadillidium decoction Experimental Study of analgesic effect of

     Abstract Objective To observe the complex Armadillidium decoction of the analgesic effect. Methods The mouse writhing induced pain, hot plate-induced pain, rat tail-flick method

    heat-induced pain stimulus experiment Armadillidium water to cook things for analgesic activity. Results Armadillidium decoction matter (protein content) 90,180,360 mg * kg-1 ig

    (ig) of acetic acid induced writhing response in mice

    significantly inhibited, the inhibitory rate of 32.00% ~ 58.15%; 30 min after gavage, and 45 min markedly improved in mice hot-plate pain threshold (P <0.01, P <0.05), the amount of analgesic effect with the dose increased in a dose-

    dependent manner. Conclusion complex Armadillidium decoction of multiple pain models are obviously inhibited in a dose-

    dependent manner.

     Key words Analgesia decoction complex Armadillidium

     Research on the Analgesic Effects of Water Decoction of Armadillidiunm vulgare in Mice

     Abstract: ObjectiveTo study the analgesic effects of water decoction of Armadillidium vulgare in mice.MethodsThe analgesic effects of water decoction from Armadillidium vulgare in mice were studied in mice and rats using writhing reaction, hot plate nociceptive test and tail flick test. ResultsThe analgesic experiment showed that protein of water decoction from Armadillidium vulgre at the dose of 90,180,360 mg * kg-1 had significant inhibition to writhing response (P <0.01, P <0.05) induced by acetic-acid.The inhibitory rate was

    from 32.00% to 58.15% . The pain threshold in mice with the hot-plate procedure increased obviously after being taken

    water decoction from Armadillidium vulgare for 30 min and 45 min (P <0.01, P <0.05). The three groups with different

    doasges could obviously increase the pain threshold in imce.The pain threshold in rats procedure increased obviously after being taken water decoction from Armadillidium vulgare for 60 min and 90 min in the tail flick test (P <0.01, P <0.05). The three groups with different dosages could obviously increase the pain threshold.ConclusionThe water decoction from Armadillidium vulgare has significant analgesic effect on animal models and shows obviously dose effect

    ralationship.

     Key words: Armadillidiam vulgare; Antalgesia; Water decoction

     Armadillidium gynecology for the rat animal dry flat beetle Armadillidiam vulgave all [1], located in Guangdong, Hebei, Shandong, Jiangsu, Zhejiang and other places. Mainly contains polysaccharide, protein, formic acid, with

    detoxification and pain relief, Poxue diuresis effect. China has been reported [1], will be late Armadillidium decoction for liver cancer pain, the role of persistent superior to pethidine, people to have for the treatment of post-operative

    pain. Are reported in the literature [2], mouse protein from women's analgesic effect. To this end, this paper Armadillidium decoction objects protein content as an indicator, using two kinds of animal models of three analgesic analgesic effect of complex Armadillidium decoction study for the further development of women and the mouse provide experimental theoretical basis for the clinical use of drugs .

     1 Materials and methods

     1.1 Drug Armadillidium dry body, purchased in Changchun

    in the Faculty of Medicine, School of Medicine identified by the Changchun. Armadillidium drying by boiling water after crushing 3 ~ 4 h, filtered, 8 000 r / min centrifugation supernatant obtained shall Armadillidium decoction objects, using Folin phenol determination of protein concentration.

     1.2 Reagent tramadol hydrochloride 50 mg / piece, by the CSPC Pharmaceutical Co., Ltd. Europa production, batch number: H1096106. Saline medicine provided by the Inner Mongolia Cogent, batch number: 030,819. Glacial acetic acid from the

north of Beijing Fine Chemicals limited liability companies.

     1.3 experimental animals 18 ~ 22 g of clean grade Kunming mice, male and female in half, from Jilin University, Center for biological experiments. Clean-level Wistar rats weighing

    180 ~ 200 g, male and female in half, from Jilin University, Center for biological experiments.

     1.4 Experimental apparatus, Huaibei, Anhui Province is China Bio-Instrument Equipment Co., Ltd.; smart hot plate instrument (YLS 6B), (HH) constant temperature water bath pan, Jiangsu Zhongshan University Instrument Factory.

     1.5 Statistical analysis of experimental data with ? s

    said that the group was used to compare between the two groups t test.

     1.6 Methods

     1.6.1 Analgesia induced by acetic acid writhing test in mice [3,4]

     Obtained 12 h fasting mice 50 (male and female half and half) were randomly divided into five groups, group 1 gavage (ig) saline, as negative control group; Group 2 gavage (ig)

    tramadol hydrochloride 15 mg / kg, as a positive control group; the first 3 to 5 groups were fed different doses of decoction complex Armadillidium by the test solution group (protein content, respectively 90,180,360 mg * kg-1) 3 doses.

    30 min after administration of each group, respectively, each mouse intraperitoneally (ip) 0.6% acetic acid 0.1 ml/10 g, observed within 20 min whether rat writhing produced by the emergence and writhing and calculates the number of twist body reduction in the number percentage (%). Temperature in the

    entire experiment (21 ? 1) ?.

     Reduction in the number writhing percentage (%) = control group, mean number of writhing - writhing test drug group mean

    writhing test drug group mean × 100% reposted elsewhere in

    the paper for free download http://www.hi138 . com

     1.6.2 mouse hot-plate method [5]

     Will be 50 female mice (screening, 5s <pain threshold

    <30s) were randomly divided equally five groups of 10 rats. Group 1 gavage (ig) saline, as negative control group; Group 2

    gavage (ig) tramadol hydrochloride 1.5 mg / kg, as a positive control group; the first 3 to 5 groups were fed different doses of rat Women decoction material by test solution (protein content, respectively 90,180,360 mg * kg-1) 3 doses.

    15,30,45, and 60 min after gavage of mice was recorded to occur into hot-plate reaction time licking hind (s) as the pain threshold. If the hot plate in mice after 60 s to stay pain-free response, should be immediately removed by 60 s calculated. Pain threshold increased percentage (%) as

    follows:

     Increased pain threshold values (%) = after treatment the average pain threshold - before treatment the average pain

    threshold before treatment the average pain threshold × 100%

     1.6.3 rat tail flick method [6]

     To (55 ? 1) ? water bath for the heat-induced pain

    source. Wistar rats were obtained after fixation, be tipped into the water and more than 5 cm in the tail of rats were recorded from the surface of the water into the water to shrink the time interval of 5 min measured twice, and pick the average tail-flick reaction time 3 ~ 10 s within the 50 rats, evenly divided male and female, were randomly divided into five groups, group 1 gavage (ig) saline, as negative control group; Group 2 gavage (ig) of tramadol hydrochloride 1 mg /

    ml, as a positive control group; the first 3 to 5 groups were fed different doses of decoction complex Armadillidium by the test solution group (protein content of 90,180,360 mg * kg-1)

    3 doses. 30,60,90,120,150 min after the administration of rats

    were measured by tail