DOC

Blood particles Guiqi Quality Standards_622

By Steve Wallace,2014-10-30 11:26
6 views 0
Blood particles Guiqi Quality Standards_622

Blood particles Guiqi Quality Standards

     Abstract Objective To establish Guiqi Blood particles (astragalus, wolfberry fruit, hemp seed, angelica, Schisandra, etc.) quality standards. Methods TLC particles in the astragalus, wolfberry fruit, hemp seed, angelica, Schisandra carried out a qualitative identification; using high-

    performance liquid chromatography - evaporative light

    scattering detection (HPLC-ELSD) was determined by preparation of astragaloside content. The results of TLC spots were clear

    astragaloside 0.500 1 ~ 10.002 μg in the context of linear

    relations, the average recovery was 98.83%, RSD = 1.13%. Conclusion The method is simple, accurate and available to the product quality control.

     Key words Blood-particle Guiqi; thin-layer chromatography;

    high-performance liquid chromatography - evaporative light

    scattering detector; Astragaloside

     Study on Quality Standard for Guiqishengxue Granules

     Abstract: ObjectiveTo establish the quality control standard of Guiqishengxue Granules (Radix Astragali, Fructus Lycii, Fructus Cannabis, Radix Angelicae Sinenis, Fructus Schisandra-e, etc.). Methods Radix Astragali, Fructus Lycii, Fructus Cannabis, Radix Angelicae Si-nenis, Fructus

    Schisandrae were identified by TLC. The content of

    Astragaloside IV was determined by HPLC-ELSD. ResultsThe TLC

    spots developed was fairly clear. The Astragaloside IV showed good linear relationship at a range of 0.500 1 ~ 10.002 μg.

    The average recovery was 98.83% with RSD was 1.13%. ConclusionThe method is simple, accurate and can be applied to the control of the products effectively.

     Key words: Guiqishengxue Granules; TLC; HPLC-ELSD;

    Astragaloside IV

     Guiqi Blood particles mainly by the astragalus, wolfberry fruit, habitat, winter days, hemp seed, angelica, peach kernel, safflower, Schisandra, etc. composed of nine Chinese herbal medicines. With spleen and kidney, Regulating the role of blood for less than spleen and kidney, qi and blood deficiency, facial skin dystrophy caused by dry skin, yellow,

    flexibility to reduce skin relaxation embolism. In order to effectively control the drug quality of the particles in the blood Guiqi Health Astragalus, wolfberry fruit, hemp seed, angelica, Schisandra qualitative study using HPLC-ELSD method,

    the main component of Astragalus Astragaloside for determination, with a view to better control of the drug quality.

     An instrument and reagent

     Instrument: Shimadzu LC-10AT, 7725i quantitative sample

    injection valve, Shimadzu C-R7Ae digital processor, the

    French-type SEDEX 55 evaporative light scattering detector (ELSD); reference substance Astragaloside (content was determined with), Chinese wolfberry, hemp seed, Angelica sinensis, Schisandra control medicines (The above reference

    substance and control medicines are purchased in China's pharmaceutical and biological products); use of chemical reagents were analytically pure, silica gel G (Qingdao Marine Chemical Plant), self-made silica gel plate (10 cm × 10 cm ×

    0.6 mm); test sample Guiqi Blood-particles (homemade);

    negative samples of prescription medicines to remove the seizure is made according to Preparation Process.

     2 Methods and Results

     2.1 Qualitative Identification of

     2.1.1 Identification of Astragalus take this product 20

    g, small study, heating water, 50 ml allows the dissolved, let cool, centrifuged and the supernatant, add ether extract three

    times, 20 ml / times, combined ether solution, and the other device to collect ; Hui to make the water layer of ether,

    adding water-saturated n-butanol extract three times, 20 ml /

    times, combined n-butanol extract, n-butanol saturated with 1%

    NaOH solution, washed three times, 20 ml / times, then n-

    butanol saturated with water, washed three times, 30 ml /

    second, n-butanol extract of the evaporated water bath set, allows the dissolved residue add 1 ml of methanol, as a solution for the test items. Another take astragalus negative samples 20 g, with the rule of law into the negative control solution. And then take Astragaloside reference substance, add methanol made from 1 mg per ml of solution, as a reference substance solution. According to thin-layer chromatography

    "Chinese Pharmacopoeia" in 2005 edition of the Ministry of

    Appendix ? VIB test, to learn for the test product solution, negative control solution of 10 μl, reference substance

    solution, 5 μl, respectively, points on the same silica gel G thin-layer plate in order to chloroform - ethyl acetate -

    methanol - water (10:20:11:5) 10 ? for the launch of the

    lower liquid agent, started out, dried, sprayed with 10% sulfuric acid ethanol solution, heated at 105 ? to spot

    obvious Color clear. Test products for chromatography, in the corresponding position with the reference substance chromatography, significantly the same color spots. Negative control samples in the corresponding position there is no such spots. Figure 1.

     2.1.2 Identification of medlar to take this product 10 g, small study, allows the dissolved 50 ml hot water, put the cold, centrifuged and the supernatant with 40 ml ethyl acetate Zhenyao extraction, extract concentration to about 1.5 ml , as a solution for the test items. An alternative medlar negative samples, with the rule of law into the negative control solution. And then take control medicines medlar 0.5 g, water 35 ml, boil 15 min, put cold, filtration, the filtrate 15 ml Zhenyao with ethyl acetate extraction, extract concentrated to about 1 ml, as control medicine solution. According to thin-

    layer chromatography "Chinese Pharmacopoeia" in 2005 edition of the Ministry of Appendix ? VIB test, to learn for the test

    product solution, negative control solution, the control of medicinal solution 5 μl, respectively, points on the same

    silica gel G thin-layer board, with toluene - ethyl acetate -

    formic acid (3:2:0.1) as the agent, started out, drying, home ultra-violet light (365 nm) under review. Test products for

chromatography, in the corresponding position control on the

    medicinal chromatography, significantly the same color of the fluorescent spots. Negative control samples in the corresponding position there is no such spots. Figure 2.

     Figure 1 Identification of Astragalus (omitted)

     Figure 2 Identification of medlar (omitted)

     2.1.3 Identification of hemp seed to make fire control medicinal hemp seed 0.5 g, ethyl vinegar 20ml, ultrasonic extraction 30 min, filtration, filtrate Hui dry ethyl 1 ml vinegar residue allows the dissolved, as the control medicine

    solution. According to thin-layer chromatography "Chinese

    Pharmacopoeia" in 2005 edition of the Ministry of Appendix ?

    VIB test, drawing a qualitative analysis for the test materials under 2.2 solution 20 μl, control medicinal

    solution, negative control solution of 5 μl, respectively, at

    the same point with carboxymethyl sodium cellulose adhesive thin-layer silica gel GF-254 board with cyclohexane - acetone

    (23:3) as the agent, started out, dried, sprayed with 10% sulfuric acid ethanol solution, at 105 ? heated to a clear

    spot color. Test products for chromatography, in the corresponding position with the control on the medicinal chromatography, significantly the same color spots. Negative control samples in the corresponding position there is no such

    spots. Figure 3.

     2.1.4 Identification of Angelica qualitative analysis of 2.1 obtained under the other device to collect the ether solution, natural Hui dry residue add 1 ml of ethanol allows the dissolved, as a solution for the test items. Another take

    Angelica negative samples, with the rule of law into the negative c