Bauhinia Extraction of total flavonoids Optimization of_970

By Jessica Russell,2014-10-30 11:15
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Bauhinia Extraction of total flavonoids Optimization of_970

Bauhinia Extraction of total flavonoids Optimization of

     Author: Xu Mei-yi Ya-Li Han Zhi-Hong Chen

     Abstract Objective To screen the Bauhinia flavonoids optimum extraction condition. Way through the water extraction, water extraction and alcohol precipitation, alcohol reference to four kinds of acid-alcohol extraction

    technique compared to determine the use of Ethanol; using L9 (34) orthogonal test to determine the optimal extraction conditions mentioned alcohol. Results Bauhinia flavonoids

    extraction conditions were optimized 15 times the amount of 55% ethanol, 100 ? water bath, each 2 h refluxing and

    extracting three times the average yield of total flavonoids 7.32%, RSD 1.44% ~ 1.88%. Conclusion optimized extraction

    process is simple, stable and feasible.

     Key words Bauhinia total flavone orthogonal experiment

     Abstract: ObjectiveTo optimize the extraction conditions of total flavonoids in Cercis chinensis Bge.MethodsBased on comparison of 4 kinds of water extraction, water extraction

    and alcohol precipitation, ethyl alcohol extraction, acid and ethyl alcohol extraction, for total flavonoids were confirmed. The parameters of ethyl alcohol extraction were performed and confirmed by L9 (34) orthogonal test. ResultsThe optimum

    extraction conditions of total flavonoids in Cercis chinensis Bge were 15 volume of 55% ethyl alcohol, 2 h hot water

    refluence at 100 ? for 3 times. Average extraction rate of total flavonoids was 7.32%, and their RSD was 1.44% ~ 1.88%.

    ConclusionThis optimized process is simple, stable and efficient.

     Key words: Cercis chinensis Bge; Total flavonoids; Extraction; Orthogonal test

     Flavonoids widespread in many plants, with diverse biological activity, especially in the treatment of

    cardiovascular disease has a strong heart, anti-myocardial

    ischemia, expand blood vessels, blood pressure, anti-

    arrhythmia, blood fat, anti-atherosclerosis and other a

    variety of roles, can effectively inhibit platelet aggregation and prevention of thrombosis [1 ~ 3]. Redbud Cercis chinensis Bge Department of legume Bauhinia flower, with heat and cooling blood, to the effect of the wind detoxification can be used to treat rheumatism, bones and muscles pain, topical treatment of nasal rickets sore, civilian applications

    bauhinia treatment of thrombocytopenic purpura [4 , 5]. Bauhinia Extraction of total flavonoids has not been reported in the literature at home and abroad, this study of total flavonoid content (yield) for the indexes, filter optimizing

    their extraction, in order to provide experimental basis for further research and development.

     An apparatus and materials

     1.1 Instrument UV 23101PC spectrophotometer (Shimadzu Corporation), FS862A type of drug smashing instrument (Zhejiang), R205B rotary evaporator (Shanghai Nobuo Technology Co., Ltd.); SUPERMODULYO-type freeze-drying machine (British

    EDWARDS companies).

     1.2 Reference Standards rutin reference standards provided by Sigma.

     1.3 Bauhinia herbs collected in Guangdong Medical College

campus, the acquisition time for the 2006 11 ~ 12. Bauhinia

    picked clean place cool well-ventilated place to dry, removal of pedicel, with stainless steel scissors will be petals Shredded, grinding, sealing the rear -20 ? to save backup.

     1.4 in all experimental reagents were analytically pure reagents.

     2 Methods and Results

     2.1 Different extraction experiments

     2.1.1 water extraction

     Bauhinia pollen that get 10 g, plus 20 times the amount of double-distilled water at 100 ? water bath, reflux 2 h,

    gauze filter, plus pairs of residues in 100 ml of distilled water at 100 ? reflux 2 h, repeated a times, combined

    filtrate, leaching. Filtrate concentrated by rotary vacuum evaporator, vacuum drying.

     2.1.2 water extraction and alcohol precipitation

     That pollen obtained 10 g, plus 20 times the amount of water, 100 ? reflux 2 h, gauze filter, together with residues in the water 100 ml at 100 ? reflux 2 h, repeated a times,

    combined filtrate, leaching. Decompression of the original volume of the filtrate concentrated to 1 / 6, by adding three times the amount of anhydrous ethanol, 4 ? to place 24 h

    precipitation, leaching, filtrate vacuum concentration, vacuum drying.

     2.1.3 Alcohol mention that pollen obtained 10 g, plus 20 times the amount of 75% ethanol, 100 ? reflux 2 h, gauze

    filter, residues in 75% ethanol plus 100 ml at 100 ? reflux 2

    h, repeated a times, combined filtrate , leaching. Filtrate

vacuum concentration, vacuum drying.

     2.1.4 acid-ethanol extracts of pollen that get 10 g, plus 20 times the amount of 75% ethanol, pH adjusted to 3 ~ 4,100 ? reflux 2 h, gauze filter, together with residues in 75% ethanol (pH3 ~ 4) 100 ml at 100 ? reflux 2 h, repeated a

    times, combined filtrate, leaching. Filtrate vacuum

    concentration, vacuum drying. Reposted elsewhere in the paper for free download http://

     2.2 Determination

     2.2.1 Preparation of standard curve [6]

     Accurate Weigh dried to constant weight of the rutin

    reference substance 5.0 mg in 25 ml flask in ethanol dissolved constant volume. Were taken 1.0,2.0,3.0,4.0,5.0,6.0,7.0 ml standard solution in 25 ml flask, add 5% sodium nitrite solution, 1 ml, shake, place 6 min, adding 10% aluminum nitrate solution, 1 ml, shaken, placed 6 min, adding 10% sodium hydroxide 10 ml, add water to the scale, shake, place 15 min. Determination of absorbance at 510 nm wavelength in order to rutin standard solution concentration (C) as the abscissa, absorbance (A) for the longitudinal coordinates, was

    the regression equation is A = 14.163C-0.004 5, the

    correlation coefficient r = 0.999 2.

     2.2.2 Determination of Flavonoids in the sample that take different approaches to accurately extract extract powder, dissolved with 70% ethanol, and fixed volume to 25 ml, respectively, absorb 3 ml in 25 ml flask in preparation of standard curve of the handle , in the measured wavelength of 510 nm absorbance, substituted into the regression equation to calculate the total flavonoid content. Total flavonoids shown

    in Table 1, single-factor analysis of variance in table 2. Table 1 Experimental results of different extraction process (slightly) Table 2 ANOVA table (omitted)

     The results can be seen from Table 1, to the yield of

    total flavonoids Health and medicine for the evaluation index, the better extraction of acid-alcohol extraction and alcohol

    references, taking into account the actual operation of the instruments on the acid-base corrosion, optimization of

    ethanol extraction process. The results can be seen from Table 2, the extraction process There was a significant difference (P

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